...) significantly increased ΔpH/min to a larger extent in K ca 3.1 +/+ than in K ca 3.1 -/- mice and dissipated the differences between the genotypes. Carbachol (100 µM) increased ΔpH/min in both genotypes but did not abolish the difference between K ca 3.1 -/- and K ca 3.1 +/+ mice. In K ca 3.1 +/+ mice the K ca...

... clamp techniques to characterize these cells and we confirm that cAMP fails to induce Cl – currents. Ca 2+ -mobilizing agents such as neurotensin (NT), ATP, and carbachol all induce a depolarization together with an increase in conductance. This was revealed in SWC and in apical membrane CAN experiments...

... -5 mol/l) raised [Ca 2+ ]i within seconds from a mean resting value of 123 ± 33 nmol/l to a peak value of 1,138 ± 304 nmol/l (n = 13). [Ca 2+ ] i returned to basal values when ATP was removed. Similarly, carbachol (CCH, 10 –5 mol/l; n = 14), neurotensin (NT, 10 –8 mol/l; n = 15), epinephrine (EPI, 10...

... the basolateral membrane in the second (‘pull’) phase via nonselective and Cl- channels. We have examined this issue recently with micromolar carbachol (CCH) concentrations in rat pancreatic acinar cells and concluded that the ‘push-pull’ model can probably not account for NaCl and fluid excretion under...

... colon and whole cell patch clamp studies in the isolated in vitro perfused rat colonic crypts. Carbachol (CCH, 100 µmol/l) enhanced Cl – secretion, quantified as equivalent short circuit current (I sc ), in rabbit colon significantly more than did prosta-glandin E 2 (PGE 2 ). In whole cell patch clamp...