... values, see also Material and Methods). Fig. 1. Percentage of RBCs (A) responding with increased intracellular Ca2+ content (elevated fluo-4 intensity) and (B) responding with increased PS exposure (annexin V-positive cells) after activation with A23187 (2 µM) for 30 min, LPA (2.5 µM) for 1 min, or PMA...

... in the figure. Fig. 4. Percentage of RBCs (A) responding with increased intracellular Ca2+ content and (B) with increased PS exposure after 30 min activation with LPA (2.5 µM) obtained from Sigma-Aldrich (batch 1). Comparison between flow cytometry (left 4 columns) and fluorescence microscopy (right 4 columns...

.... There are no significant differences between the fractions, ANOVA, p > 0.05. Fig. 5. Percentage of RBCs responding with increased intracellular Ca2+ content from different fractions (cells separated by age) after activation with A23187 (2 µM) using flow cytometry depending on time. Non-separated RBCs, young cells...

...): untreated RBCs; right curve (grey): RBCs after LPA stimulation. A small population of WRR and a large population of SRR can be observed. Fig. 1. Histograms of flow cytometry analysis of intracellular Ca2+ content in RBCs. (A) Representative histogram showing the classification of RBCs into two populations...