...-62, 1-[N,O-bis-(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine; p-CAMKⅡ,Ca2+/Calmodulin-Dependent Kinase II phosphorylation. Interestingly, p-CAMKII protein, immunolocalized by immunohistochemistry in articular cartilage, was positively associated with the corresponding OA...

.... In addition, a low-frequency rhythmicity was observed as indicated by the dashed line. (B) Comparison of the frequency of the oscillatory contractions and the rhythmicity. ***: P< 0.01. Fig. 7. TEA induces Ca2+ oscillations in ASMCs cells. (A) ACH induced an increase in Ca2+ concentration in ASM cells...

... of intracellular Ca2+ by fluo-4AM staining. Chondrocytes were washed twice with PBS containing 5% FCS and resuspended in 500 µl binding buffer provided in the detection kit, followed by incubation with 5 µl annexin V-FITC and 5 µl propidium iodide (PI) at room temperature for 15 min in the dark. Flow cytometry...

... for commercial purposes as well as any distribution of modified material requires written permission. Original Paper 319 Transient Receptor Potential Vanilloid 5 Mediates Ca2+ Influx and Inhibits Chondrocyte Autophagy in a Rat Osteoarthritis Model Yingliang Wei Yanfang Wang Yutong Wang Lunhao Bai Department...

... that CHQ-induced enhancement of GLUT4 trafficking to the PM is not dependent on CHQ-triggered intracellular Ca 2+ increase. Fig. 3. BAPTA-AM did not inhibit CHQ-stimulated GLUT4 trafficking in L6 cells. (A) Cells were treated with 1 mM CHQ. Changes in intracellular Ca2+ (upper) and IRAP fluorescent...

...”. Phosphatidylserine Ca2+ Phospholipase A2 ATP Eryptosis The present study explored the effect of clofazimine on eryptosis, the suicidal erythrocyte death. The most important hallmark of eryptosis is cell membrane scrambling with phosphatidylserine translocation to the cell surface. Phosphatidylserine...

... osteoclast differentiation. Summarized data showed that the time-dependent decrease in TRPV6 expression during osteoclast differentiation. n = 3, *P < 0.05, **P <0.01. H and I: [Ca2+]i changes were traced in TRPV6+/+ and TRPV6-/- BMMs treated with M-CSF for 72 hr [Ca2+]i. Changes were estimated...

... formation (one-way ANOVA). Ionomycin (1 µM) was used as a positive control and DMSO as the solvent control. Fig. 2. Effect of vancomycin on cytosolic Ca2+ activity and ceramide formation in human platelets. A. Representative histogram of Fluo3 fluoresence following a 30 minutes exposure to Tyrode buffer 2...

... by Extracellular Ca2+ and is Tetanus Toxin Resistant Martina Orecná1, Roman Hafko1, Veronika Toporcerová1, Vladimír trbák1,2 and Zuzana Bacová1,2 1Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, 2Department of Pathological Physiology, Medical Faculty, Slovak Medical University...

..., University of Oslo, Oslo Key Words LRF-1/ATF3 Hepatocytes G protein-coupled receptors Vasopressin Ca2+ Mitogen-activated protein kinases Abstract Background/Aims: Liver regeneration factor 1 (LRF1/ATF3) is an early response gene which is rapidly induced upon partial hepatectomy in rats...

...:91-102 and Biochemistry Accepted: July 26, 2009 2-APB and Capsazepine-induced Ca2+ Influx Stimulates Clathrin-dependent Endocytosis in Alveolar Epithelial Cells Shariq M. Usmani1, Giorgio Fois1, Susanne Albrecht1, Sonja von Aulock2, Paul Dietl1 and Oliver H. Wittekindt1 1Institute of General...

... of Sciences, Bratislava, 2Department of Pathological Physiology, Faculty of Medical Specialty Studies, Slovak Medical University, Bratislava, 3Polymer Institute, Slovak Academy of Sciences, Bratislava Key Words Insulin secretion Ethanol Ca2+ PKC PTP SNARE Abstract Alcohol causes reactive...

... in a contrasting manner. Maximal activities of Na + -K + ATPase and K + channel are achieved with alkalosis in the presence of Ca 2+ . In contrast, K + transport is reduced to a minimum with acidosis in the absence of Ca 2+ . Ca2+ Diluting segment, frog kidney K+ channel K+ transport Na+-K+pump pH 6...