... of endothelial cells (ECs) in vascular calcification of hypertension remains unclear. Therefore, the present study explored the effects of ECs on calcification of smooth muscle cells (SMCs) from aortas of spontaneously hypertensive rats (SHR). Methods: Aortic ECs and SMCs were isolated from SHR and Wistar rats...

... - ] i ) measurements to investigate the mechanism underlying phenylephrine (PE)-induced rhythmic contraction in the mouse aorta. Results: We found that Na + -K + -2Cl - cotransporter 1 (NKCC1) inhibitor bumetanide abolished PE-induced rhythmic contraction. The Cl - channel blockers DIDS and niflumic...

... with speed and progressive load. After training, blood samples were collected for biochemical assessments and the aorta was removed for dissection and histological morphometry study. In addition, the expression of angiotensin-converting enzyme (ACE) and angiotensin II proteins were examined...

...) and Cx37, which allow the exchange of signaling molecules to coordinate their activity. Here, we investigated the role of Cx40 in the endothelial Ca 2+ signaling of the mouse aorta. Methods: Ca 2+ imaging was performed on intact aortic endothelium from both wild type (Cx40+/+) and Connexin40-deficient...

... with higher collagen content (control: 2.13±0.40 vs. 3.46±0.36 and LDLR -/- : 2.37±0.36 vs. 3.79±0.60; AU p<0.05 red Sirius staining). Interestingly, ROS production in the aorta was only increased in the LDLR -/- +cholesterol group (0.17±0.04 and 0.16±0.05 in the control groups, 0.14±0.02 vs. 0.34±0.06...