...Wei Ding; Tingyan Liu; Xiao Bi; Zhiling Zhang Background/Aims: Growing evidence suggests mitochondrial dysfunction (MtD) and the Nlrp3 inflammasome play critical roles in chronic kidney disease (CKD) progression. We previously reported that Aldosterone (Aldo)-induced renal injury in vitro...

... and inflammatory cytokines were detected by RT-PCR. The protein levels of CTRP1, aldosterone synthase (CYP11B2) and mitogen-activated protein kinase were examined by Western blotting. Results: The levels of CTRP1 in the plasma and EAT were higher in the CHF patients than those in the controls. There were...

... treatment with exogenous hydrogen peroxide (H 2 O 2 ) and aldosterone in cultured HUVECs. Cell viability decreased upon treatment with both agents in a concentration-dependent manner, which could be partially reversed by 9-phenanthrol, a specific TRPM4 inhibitor. Exogenous H 2 O 2 induced apoptosis...

...Bin Wang; Xianlin Xu; Xiaozhou He; Zhigang Wang; Min Yang Background/Aims: Berberine, a naturally occurring isoquinoline alkaloid, acts against oxidative stress (OS) and endoplasmic reticulum stress (ERS), both of which are responsible for Aldosterone (Aldo) -induced podocyte injury. However...

...Bin Wang; Wei Ding; Minmin Zhang; Hongmei Li; Yong Gu Background/Aim: Aldosterone (Aldo), a mediator of kidney fibrosis, is implicated in the pathogenesis of chronic kidney diseases (CKD). The aim of this study was to evaluate the regulatory role of rapamycin (Rap) in Aldo-induced...

... of this study is to clarify the effect of Cx43 in aldosterone (Aldo)-induced podocyte injury and explore the possible molecular mechanism behind this effect. Methods: Uninephrectomized Sprague-Dawley rats were given 1% NaCl (salt) in their water and an Aldo infusion (0.75 μg/h) for 28 days to induce podocyte...

... and transcriptional activity. Previously, we have shown that MR is monoubiquitylated at the basal state and that aldosterone stimulation induces monoubiquitylation removal prompting polyubiquitin-dependent destabilization of the receptor and proteasomal degradation. This study investigated the role of the aldosterone...

... reabsorption, iodide accumulation and endolymph ion balance, respectively. The molecular mechanisms controlling pendrin activity in renal, thyroid and inner ear epithelia have been the subject of recent studies. The effects of ambient pH, the hormone aldosterone and the peptide uroguanylin (UGN...

...Hans Oberleithner Madin-Darby canine kidney cells resemble the intercalated cells of the renal collecting duct. In these cultured epithelial cells, aldosterone activates apical Na + /H + exchange, initiating a cascade of intracellular events like cell growth, epithelial cell polarity...

... ± 0.53. Aldosterone increased Na + /K + -ATPase activity in IMCD i and IMCD m monolayer cultures by 90 and 65 %, respectively, after 48 h of incubation. Dexamethasone increased the enzyme activity in IMCD i and IMCD m by 78 and 85%, respectively. These data indicate that IMCD subsegmental cell...

... to transmembrane HCO – 3 shifts. Each voltage spike is followed by a transient period of intracellular alkalosis inactivating spiking activity. Aldosterone blunts oscillations through stimulation of plasma membrane Na + /H + exchange and subsequent intracellular alkalinization. The pH changes may play an important...

... death, probably by alteration of the membrane ionic permeability. This indicates that the Na + channel is part of a new family of cation channels. The epithelial Na + channel is regulated by hormonal and intracellular factors. Aldosterone increases the open probability of channels present in the apical...

...Hans-Jürgen Westphale; Christof Schäfer; Hans Oberleithner Aldosterone leads to an increase in urinary K + secretion. To test for the cellular mechanism underlying K + transport in the early distal tubule of the amphibian kidney, the K + activity in the lumen of this segment (K a lu ) was measured...

...Lyubomira Ivanova; Rita Bernhardt; Ingolf Bernhardt The direct influence of aldosterone on the human and avian red blood cell (RBC) transport systems, Na + /K + pump, Na + ,K + ,2Cl - symporter, and K + (Na + )/H + exchanger, was investigated with tracer kinetics. The present work proved...