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Cellular Physiology and Biochemistry (2018) 51 (4): 1852–1862.
Published Online: 30 November 2018
... myocardial cells. Methods: CNTF and CNTFR expression were tested by RT-PCR assay and Western blotting assay. Cell viability and death were tested by MTT assay and LDH release assay, respectively. Akt-Nrf2 signalings were tested by Western blotting assay and qPCR assay. Results: CNTF and its receptor CNTFR...
Xiaofang Wang, Yuan Liu, Lili Xiao, Ling Li, Xiaoyan Zhao, Lulu Yang, Ning Chen, Lu Gao, Jinying Zhang
Cellular Physiology and Biochemistry (2018) 51 (2): 827–841.
Published Online: 22 November 2018
... hypertrophy was mediated by blocking activation of the AKT signaling pathway. Conclusion: Hyp improves cardiac function and prevents the development of cardiac hypertrophy via AKT signaling. Our results suggest a protective effect of Hyp on pressure overload-induced cardiac remodeling. Taken together, Hyp may...
Cellular Physiology and Biochemistry (2018) 50 (2): 798–809.
Published Online: 11 October 2018
.... Further studies show that AKT could be the primary resistance factor of AZD5153. Pharmacological inhibition or genetic depletion of AKT induced BRD4 downregulation, sensitizing AZD5153-induced cytotoxicity in PC-3 cells. In vivo , AZD5153 oral administration inhibited PC-3 xenograft tumor growth in nude...
Cellular Physiology and Biochemistry (2018) 50 (2): 745–756.
Published Online: 11 October 2018
... of autophagy activator rapamycin and was dramatically counteracted by 3-methyladenine, an autophagy inhibitor. Furthermore, PGE1 suppressed the phosphorylation of AKT and mTOR, which negatively regulated autophagy level in VSMCs. In vivo study, PGE1 remarkably improved the endothelium-independent contraction...
Cellular Physiology and Biochemistry (2018) 50 (1): 121–135.
Published Online: 02 October 2018
... by reducing the PKC-dependent AKT activity and elevating JNK phosphorylation. Since it was thought that GqPCR induces mainly activation of AKT, we undertook to examine how general is this phenomenon and understand its signaling. Methods: We used various cells to follow the phosphorylation of signaling...
Cellular Physiology and Biochemistry (2018) 49 (5): 2073–2087.
Published Online: 21 September 2018
... apoptosis under HG and/or hypoxic conditions by facilitating the expression of protein phosphatase-1 nuclear targeting subunit (PNUTS), X-linked inhibitor of apoptosis protein (XIAP), p-AKT, p-Bad, increased the Bcl-2/Bax ratio, as well as downregulated cleaved caspase 3 expression. Mechanistically, we...
Xiao-Zhi Bai, Ju-Lei Zhang, Yang Liu, Wei Zhang, Xiao-Qiang Li, Ke-Jia Wang, Meng-Yuan Cao, Jia-Ning Zhang, Fu Han, Ji-Hong Shi, Da-Hai Hu
Cellular Physiology and Biochemistry (2018) 49 (2): 489–500.
Published Online: 29 August 2018
... study showed that microRNA-138 was increased in macrophages stimulated with LPS. Additionally, the NF-κB and AKT pathways were both activated. The pre-treatment of microRNA-138 inhibitor decreased inflammatory factors, downregulated the NF-κB pathway, activated the AKT pathway and protected against...
Aijun Yang, Yanzhu Lu, Junchao Xing, Zhilin Li, Xiaolong Yin, Ce Dou, Shiwu Dong, Fei Luo, Zhao Xie, Tianyong Hou, Jianzhong Xu
Cellular Physiology and Biochemistry (2018) 48 (1): 361–370.
Published Online: 17 July 2018
.../Akt on the migration potential of hBMSCs. The in vitro chondrogenic differentiation of hBMSCs was assessed by examination of 2 chondrogenic markers, Sox9 and type 2 collagen (COL2). mBMSCs were used in tissue engineered bone (TEB) with/without IL-8 implanted into bone defect area with CXCR2 or Akt...
Wenqian Feng, Yancheng Wu, Guangxin Chen, Shoupeng Fu, Bai Li, Bingxu Huang, Dali Wang, Wei Wang, Juxiong Liu
Cellular Physiology and Biochemistry (2018) 47 (4): 1617–1629.
Published Online: 27 June 2018
... Liquid Chromatography, the expression levels of tight junction proteins in the intestinal tract and related signaling molecules, such as GPR109A and Akt, in the colon were examined by quantitative real-time PCR or western blot analyses on day 21. Caco-2 cells were used as a colon cell model and cultured...
Cellular Physiology and Biochemistry (2018) 47 (2): 604–616.
Published Online: 22 May 2018
... were performed to measure the cellular responses to drug treatment. Western blotting and ELISA were performed. The expression of Cleaved- Casepase 3, Beclin-1, p62 and LC3I/II were quantified, and the Phosphorylation of eNOS and Akt were evaluated. The ratio of Bcl-2/ Bax was determined to reveal...
Pu Wang, Yu Cheng Lu, Jie Wang, Lan Wang, Hanry Yu, Yuan Fei Li, Alice Kong, Juliana Chan, Shaochin Lee
Cellular Physiology and Biochemistry (2018) 47 (1): 356–367.
Published Online: 11 May 2018
... and palmitic acid, alone or in combinations, induced ROS production and centrosome amplification. Together, they increased AKT activation as well as the expression, binding and centrosome translation of ROCK1 and 14-3-3σ. Results from further analyses showed that AKT-ROS-dependent upregulations of expression...
Cellular Physiology and Biochemistry (2018) 46 (4): 1628–1642.
Published Online: 20 April 2018
...-κBp65, p53, Bax and PCNA were detected by Immunohistochemistry. To gain an insight into the molecular signaling mechanisms, we determined the effect of CM on MAPKs, NF-κB and PI3K/Akt/eNOS pathways by Western blotting. Results: CM lowered 5-FU-triggered increase of creatinine, BUN, Kim-1 and NGAL renal...
Bo-pei Li, Yuan-tian Mao, Zhen Wang, Ye-yang Chen, Ye Wang, Chong-yu Zhai, Bo Shi, Si-yu Liu, Jin-lu Liu, Jun-qiang Chen
Cellular Physiology and Biochemistry (2018) 46 (3): 907–924.
Published Online: 13 April 2018
... increased. Further study showed that the expression levels of ITGα3, ITGαv and ITGβ1, as well as AKT-phosphorylation, ERK-phosphorylation and p38-phosphorylation, were reduced in vivo after CLIC1 knockdown, while ITGα1 was upregulated. Conclusions: We speculate that CLIC1 may play an important role...
Ning Wang, Yang Lu, Kui Wang, Wei-song Li, Pan Lu, Shan Lei, Rong Li, Hong Zhang, Juan Zheng, Hai-xia Lu, Xin-lin Chen, Yong Liu, Peng-bo Zhang
Cellular Physiology and Biochemistry (2018) 46 (2): 618–632.
Published Online: 28 March 2018
... and apoptosis were assessed by immunohistochemistry, CCK-8 and TUNEL, respectively. The protein expressions of caspase-3, p-Akt and p-GSK-3β both in vivo and vitro were assessed by western blotting. Cognitive functions were assessed by Morris Water Maze test and context fear conditioning test at the adult...
Cellular Physiology and Biochemistry (2018) 45 (5): 2136–2144.
Published Online: 07 March 2018
...) and TUNEL staining were conducted to evaluate the effect of choline on cardiac apoptosis and autophagy. Protein levels of autophagic markers including LC3, beclin-1 and p62 as well as Akt and mammalian target of rapamycin (mTOR) were examined by Western blotting. Results: Myocardial IR-induced cardiac...
Xuelian Li, GuoYuan Wang, MuGe QiLi, HaiHai Liang, TianShi Li, XiaoQiang E, Ying Feng, Ying Zhang, Xiao Liu, Ming Qian, BoZhi Xu, ZhiHang Shen, Samuel Chege Gitau, DanDan Zhao, HongLi Shan
Cellular Physiology and Biochemistry (2018) 45 (5): 1955–1965.
Published Online: 02 March 2018
... were used to detect the effects of aspirin on cardiac fibrosis in vivo and in vitro . Western blot and qRT-PCR were applied to examine the impact of aspirin on extracellular signal-regulated kinases (Erks), p-Akt/β-catenin, SerpinE2, collagen I, and collagen III levels in the mice heart. Results...
Zongjuan Li, Xiangdong Xu, Yizhuo Li, Kun Zou, Zhuo Zhang, Xiaoying Xu, Yina Liao, Xinrui Zhao, Wei Jiang, Wendan Yu, Wei Guo, Yiming Chen, Yixin Li, Miao Chen, Wu-guo Deng, Liren Li, Lijuan Zou
Cellular Physiology and Biochemistry (2018) 45 (5): 1772–1786.
Published Online: 23 February 2018
... enhanced the inhibitions of cell viability, colony formation, migration and invasion, and the induction of apoptosis in thyroid cell lines, and synergistically suppressed tumor xenograft growth by inhibiting the PI3K/Akt/mTOR and EMT signaling pathways, up-regulating p53 targeted genes, and triggering...
Zhijian Jin, Xi Cheng, Haoran Feng, Jie Kuang, Weiping Yang, Chenghong Peng, Baiyong Shen, Weihua Qiu
Cellular Physiology and Biochemistry (2017) 44 (4): 1471–1484.
Published Online: 01 December 2017
... in ATC cells. Angiogenesis antibodies array was utilized to detect the expression of angiogenesis-related genes after Apatinib treatment in ATC cells. In addition, we used Akt activator, Akt inhibitor and GSK3β inhibitor to further study the mechanism for how Apatinib suppressed angiogenesis. Results...
Cellular Physiology and Biochemistry (2017) 44 (3): 1188–1198.
Published Online: 28 November 2017
... as a competing endogenous RNA (ceRNA) for miR-21 and miR-590-5p. These interactions led to repression of AKT pathway and inhibition of HCC cell proliferation and migration. Conclusion: Thus, our data showed that Linc-USP16, as a tumour suppressor, plays an important role in HCC pathogenesis and provides a new...
Yaoguo Lang, Xianglong Kong, Changjun He, Fengjiao Wang, Benkun Liu, Shibo Zhang, Jinfeng Ning, Kaibin Zhu, Shidong Xu
Cellular Physiology and Biochemistry (2017) 44 (2): 455–466.
Published Online: 15 November 2017
... the sensitivity to chemotherapy drugs in NSCLC cells. Importantly, we found that MSI1 could regulate the activity of Akt signaling. The regulation of NSCLC proliferation, glucose metabolism and chemoresistance by MSI1 was dependent on the modulation of the activity of the Akt signaling pathway. We also found...