... that the levels of energy metabolites, such as the high-energy phosphate pool (ATP and creatine), significantly decreased in a time-dependent manner. Correlation analysis revealed the inhibition of glycolysis and the tricarboxylic acid (TCA) cycle, enhanced lipid metabolism, and changes in some amino acids, which...

... was not obviously affected during macrophage differentiation. Conclusion: In this study, we demonstrated that neutrophil differentiation is regulated by ER stress/UPR that is supported by mitochondrial ATP supply, in which IRE1α-XBP1 activation is essential. Our findings provide the evidence that mitochondrial...

... progression in the kidney is poorly understood. This study examines a role for glucose-evoked changes in the beta1 isoform of transforming growth factor (TGFβ1), on connexin expression, gap-junction mediated intercellular communication (GJIC) and hemi-channel ATP release from tubular epithelial cells...

...Xia Wang; Long Li; Ruijuan Guan; Danian Zhu; Nana Song; Linlin Shen Background/Aims: Extracellular ATP performs multiple important functions via activation of P2 receptors on the cell surface. P2Y receptors play critical roles in ATP evoked response in human lung adenocarcinoma cells (A549 cells...

... receptors (SURs) of adenosine triphosphate-sensitive potassium channels (K ATP ). Glibenclamide may be also cardiotoxic, this effect being ascribed to interference with the protective function of cardiac K ATP channels for which glibenclamide has high affinity. Prompted by recent evidence that glibenclamide...

... and extracellular ATP with fluorometric assay kit were used to analyze the changes in the intracellular calcium concentration induced by calcium channel opening and the release of ATP, respectively, at different operation times. Results: When the apparent strain reached 10000 µε, the strain scope of fber at levels...

...+ activity ([Ca 2+ ] i ) from Fluo3-fluorescence, reactive oxygen species (ROS) from 2′, 7′-dichlorodihydrofluorescein diacetate (DCFDA) fluorescence, and cytosolic ATP level utilizing a luciferin-luciferase assay kit. Results: A 24-48 hours exposure of human erythrocytes to clofazimine (≥1.5 µg/ml...

... in dendritic cells (DCs), antigen presenting cells involved in the initiation and regulation of antigen-specific T-cell responses. Dendritic cell function is regulated by cytosolic Ca 2+ activity ([Ca 2+ ] i ). Mediators increasing [Ca 2+ ] i in DCs include ATP and the chemokine receptor CXCR4 ligand CXCL12...

... and ibuprofen treatment reversed the reduced production of glucose, pyruvate, lactate and urea from alanine as well as the reduced production of glucose from pyruvate and lactate in perfused liver from tumor-bearing rats. Besides, celecoxib and ibuprofen treatment restored the decreased ATP content, increased...

... + /K + -ATPase protein abundance by flow cytometry or immunofluorescence, and cellular ATP by luciferase-assay in DCs from bone marrow of JAK3-knockout ( jak3 -/- ) or wild-type mice ( jak3 +/+ ). I pump was further determined by voltage clamp in Xenopus oocytes expressing JAK3, active A568V JAK3...

...) and fed blood glucose in dose-dependent manner, as well as reduced 24-h food and water intake. In vitro, SalA caused dose-dependent increase in glucose consumption and enhanced glucose uptake. SalA significantly increased ATP production from 10 min to 12 h in HepG2 cells and L6 myotubes. Interestingly...

... a series of in vitro experiments to examine whether a positive autocrine feedback loop existed between microglia-derived BDNF and subsequent microglial activation as well as the mechanisms underlying this positive feedback loop. Methods: Because ATP is a classic inducer of microglial activation, firstly...

... velocity (VSL), curvilinear velocity (VCL), and average path velocity (VAP) were recorded. Intracellular free calcium concentration ([Ca 2+ ] i ), pH value (pHi), and ATP content were determined. Results: Co-incubation with anti-VDAC antibody reduced VSL, VCL, and VAP of spermatozoa. Co-incubation further...

...Bernadett Balázs; Tamás Dankó; Gergely Kovács; László Köles; Matthias A. Hediger; Ákos Zsembery Background/Aims: ATP-gated P2X 4 purinergic receptors (P2X 4 Rs) are cation channels with important roles in diverse cell types. To date, lack of specific inhibitors has hampered investigations on P2X 4...

... in the presence of an ATP-degrading enzyme, apyrase (10 U/ml). Application of ATP (100 µM) under isotonic conditions generated the current through VSOR channels via Ca 2+ nanodomains, as bradykinin does. The current induced by the hypotonic stimulus was suppressed by ∼40% in the Ca 2+ -depleted condition where...

... - exchanger NDCBE to mediate NaCl reabsorption in B-ICs. Second, pendrin activity regulates Na + reabsorption by the adjacent principal cells, possibly by interaction with the ATP-mediated paracrine signalling recently identified between ICs and principal cells. Interestingly, the water channel AQP5...

... proteins in islets and Beta-TC6 cells was confirmed by immunofluorescent staining and Western blot analysis. We have previously found that the functional P2Y 1 receptor but not P2Y 2 and P2Y 4 receptors was present in islets. In this study we found that a nonspecific P2 receptor agonist, ATP (1 µM...

...Takahiro Yaguchi; Masaru Saito; Yoshiyuki Yasuda; Takeshi Kanno; Takashi Nakano; Tomoyuki Nishizaki Background/Aims: Adenosine 5′-triphosphate (ATP) mediates a variety of signal transductions via ATP receptors such as P2X and P2Y receptors. The present study aimed at understanding the mechanism...

... is remarkable in several aspects, considering stimulus-delayed fusion activity, poor solubility of vesicle contents, long hemifusion lifetimes, slow fusion pore expansion and active, actin-driven content release. Cell stretch as well as P2Y 2 receptor stimulation by extracellular ATP are considered the most...

..., instructions or products referred to in the content or advertisements. Alveolar type II cells ATP Lamellar bodies Plasma membrane Endocytosis Cellular Physiology and Biochemistry Original Paper Cell Physiol Biochem 2010;25:81-90 Accepted: September 08, 2009 Plasma Membrane Trafficking in Alveolar...