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Keywords: 2-DE
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Journal Articles
Journal:
Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2013) 31 (2-3): 408–420.
Published Online: 12 March 2013
... (http://wwwmatrixscience. com). In this study, the levels of six proteins identified on a 2-DE map of rat muscle were verified by immunoblot analysis. Tissue lysates were prepared with RIPA buffer (Sigma), homogenized, and centrifuged at 12,000 ×g for 20 min. Extract was diluted in 5X sample...
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Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2012) 29 (3-4): 617–634.
Published Online: 03 April 2012
..., and abdominal fat deposits of both genders in lean and obese rats fed a high fat diet (HFD) using a combination of 2-DE and MALDI-TOF-MS. Results: The proteomics analysis enabled us to detect 25, 29, and 46 proteins showing gender differences in three WAT deposits, respectively, to gain insight into cause...
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Cellular Physiology and Biochemistry
Cellular Physiology and Biochemistry (2011) 28 (5): 933–948.
Published Online: 15 December 2011
... performed differential proteome analysis of BAT from lean and obese rats of both genders fed a HFD using 2-DE combined with MALDI-TOF-MS. Results: When exposed to a HFD, male rats gained more body weight with increased values of plasma biochemical parameters than did female rats. Among 595 matched spots, 48...