Subsequent to osmotic swelling, the nucleated erythrocytes of the flounder (Platichthys flesus) displayed a volume-regulatory decrease (RVD) which was similar in HEPES and HCO3 media. This RVD was characterized by net K+, Cl and water loss at constant Na+. During RVD, 46% of the net water flow was secondary to net inorganic ion flux in HEPES compared to about 77% in HCO-3 media, the resting might be attributed to organic osmolytes. In the presence of added bicarbonate ions at physiological concentrations and oxygen in the experimental medium, not only a K+/C1- cotransport seems to be involved during RVD, but also K+ and Cl conductances. Moreover, transport pathways sensitive to N-phenylanthronilic acid or 4-acetamido-4’-isothyocyanate-2, 2-stilbene di-sulfonic acid play an important role in the volume-dependent ionic movements and in the volume-activated release of taurine. These results strongly suggest that the anion transporter (band 3) or another neighboring protein plays an important part in RVD in volume-activated osmolyte release after hyposmotic stress in flounder red blood cells.

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