We have identified a h SK3-transcript, h SK3_ex1c, which is generated by alternative splicing. Isoform h SK3_ex1c lacks the cytosolic N-terminus and the first transmembrane helix and is exclusively expressed in non-neuronal tissues. h SK3 transfected tsA cells showed a Ca2+-activated K+ current in patch-clamp experiments, whereas h SK3_ex1c transfected cells and cells co-transfected with both isoforms did not. We fused both isoforms to fluorescence proteins and observed h SK3 localization predominantly in the plasma membrane. The co-expression of h SK3 + h SK3_ex1c resulted in their cytoplasmic co-localization. Thus, h SK3_ex1c has a dominant-negative effect on h SK3 by preventing its transport into the plasma membrane.

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© 2004 S. Karger AG, Basel
2004
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