... of immunodiffusion assay. Down to a C1-INH level of approximately 0.075 g/1(38% of normal) apparent C1-INH functions were found within the normal range, while below this level functional adequacy of C1-INH could no longer be ascertained. When C4 concentrations, considered to reflect approximately functional C1-INH...

...Alister W. Dodds; S.K. Alex Law We have previously noted a large difference in the specificity of the covalent binding reaction of human C4-A and C4-B. Here we report data on three other thioester- containing proteins. Human C3 is unreactive with glycine but its reactivity with glycerol (kˊko...

...Dominic G. Spinella; Dulari D. Shah; Pamela D. Hale; R. Paul Levine A C4-specific monoclonal antibody has been developed that allows the rapid purification of C4 from whole serum or plasma by affinity chromatography. 1- to 2-ml volumes of serum are loaded onto a small column of antibody-coupled...

...J.M. Alcolea; L.C. Antón; G. Marqués; P. Sánchez-Corral; F. Vivanco The binding properties of activated C4 to immune complexes (ovalbumin-rabbit IgG antiovalbumin) were studied by using I-IgG in the immune complexes or performing the C4 binding assays in the presence of 14C-iodoacetamide. High...

...D.M. Groth; J.D. Wetherall; B.A. Umotong; P. Sparrow; I.R. Lee; M.J. Carrick A relatively rapid method for the isolation of complement protein C4 from bovine plasma is described. The method consists of DEAE Sephacel anion exchange chromatography of plasminogen-depleted bovine plasma followed...

...John N. Goldman; Kathleen S. O’Rourke; John D. McMannis; Margaret B. Goldman We have previously shown that administration of anti-C4 antibody to cells in culture can suppress the synthesis and secretion of C4. Lymphoid cells must be present along with the C4 secreting macrophages to achieve...

...Chester Alper; Zuheir Awdeh; Edmond Yunis The region on the short arm of the sixth human chromosome encoding class I and class II histocompatibility antigens involved in immune recognition also encodes a group of molecules unrelated to HLA termed class III which includes C2, C4, and factor B...

...E. Sim A range of drugs including hydralazine, isoniazid, procainamide and penicillamine cause toxic side effects which resemble systemic lupus erythematosus (SLE). Deficiencies of Cl, C4 and C2 are associated with idiopathic SLE, and these defects may compromise the ability of the patient to deal...

... for Clq, Clr, Cls, C4 and C2 and summarizes monoclonal antibodies reported so far which recognize either conserved epitopes or activation-dependent epitopes with particular emphasis on neoepitopes occurring during the activation cascade. 11 8 2017 1989 Copyright / Drug Dosage / Disclaimer...

...Maria A. Picchi; Bonnie M. Bradt; Neil R. Cooper; Ronald T. Ogata Previously cloned and sequenced full-length cDNAs for murine C4 and the closely related sex-limited protein (Sip) have been placed into an eucaryotic expression vector. Transfer of these DNA constructs transiently into monkey COS...

...G. Mauff; Martina Brenden; Margot Braun-Stilwell; Gabriele Doxiadis; Carolyn M. Giles; G. Hauptmann; C. Rittner; P.M. Schneider; Beate Stradmann-Bellinghausen; Beatrice Uring-Lambert Human C4 is most polymorphic at the protein level, distinction between allotypes of the C4A and C4B proteins resting...

...Brian D. Reilly; Paul Levine; Jonathan Rothbard; Verna M. Skanes Comparison of amino acid sequences of the a-chain fragment of human C4, C4d, has shown C4A- and C4B-specific sequences at residues 1101-1106 in which the aspartic acid-histidine substitution at position 1106 may be related...

...G. Mauff; C.A. Alper; R. Dawkins; G. Doxiadis; C.M. Giles; G. Hauptmann; C. Rittner; P.M. Schneider A common and revised nomenclature of the allotypes of the fourth component (C4) of human complement has been proposed. It is based on the results of the C4 Reference Typing of the Vlth Complement...

...G. Mauff; Martina Brenden; Margot Braun-Stilwell For the definition of common C4 allotypes relative electrophoretic migration (RM) values were determined. A set of standard C4 variants were investigated by prolonged agarose gel electrophoresis and subsequent immunofixation with specific antiserum...

...Thomas Kaufmann; Hans-Joachim Kytzia; Christian Rittner Based on a method that uses the fourth component of complement (C4)-defi-cient guinea pig serum to quantify the hemolytic activity of C4, we developed an assay that allows the processing of a large number of individual samples with high...

... or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Autoimmune disease C2 C4 C7 C8 Infection C9 Complement deficiency Original Papers...

...Saija Koskimies; Marja-Liisa Lokki; Krister Höckerstedt Plasma complement allotypes for C4 and factor B were determined in 21 patients before and after liver transplantation together with donor typing. In 17 transplantations allelic differences between the patient and the donor could be shown...

...Akira Miyano; Hiroyuki Kitajima; Futoshi Fujiwara; Masahiro Nakayama; Tomio Fujita; Masanori Fujimura; Toru Takeuchi; Akira Shimizu The levels of CH50, the complement components, C1q, C4, and C3, C3 degradation fragments, and factor B were determined in the cord blood of 128 newborn infants...