Abstract
Background: New effective, economical and safe antimalarial drugs are urgently needed due to the development of multi-drug-resistant strains of the parasite. Homeopathy uses ultra-diluted doses of various substances to stimulate autoregulatory and self-healing processes to cure various ailments. The aim of the study was to evaluate the in vitro and in vivo antimalarial efficacy of a homeopathic drug, Chininum sulphuricum 30C. Methods: In vitro antiplasmodial activity was screened against the P. falciparum chloroquine-sensitive (3D7) strain, and cell viability was assessed against normal human dermal fibroblasts and HepG2 cells. Suppressive, preventive and curative studies were carried out against P. berghei-infected mice in vivo. Results: Chininum sulphuricum (30C) revealed good antiplasmodial activity in vitro, with 92.79 ± 6.93% inhibition against the 3D7 strain. The cell viability was 83.6 ± 0.6% against normal human dermal fibroblasts and 95.22 ± 5.1% against HepG2 cells. It also exhibited suppressive efficacy with 95.56% chemosuppression on day 7 with no mortality throughout the follow-up period of 28 days. It also showed preventive activity against the disease. Drug treatment was also safe to the liver and kidney function of the host as evidenced by biochemical studies. Conclusion: Chininum sulphuricum 30C exhibited considerable antimalarial activity along with safety to the liver and kidney function of the host.
References
1.
de Souza
JB
, Riley
EM
. Cerebral malaria: the contribution of studies in animal models to our understanding of immunopathogenesis
. Microbes Infect
. 2002
Mar
;4
(3
):291
–300
.
[PubMed]
1286-45792.
World Health Organization (WHO)
. Global Malaria Report.
2019
. ISBN 978 92 4 156572 1.3.
World Health Organization (WHO)
. Guidelines for the treatment of malaria.
3rd Ed, 2015
. ISBN 978 92 4 154912.4.
World Health Organization (WHO)
. Withdrawal of oral artemisinin-based monotherapies
, (http://www.who.int/malaria/areas/treatment/withdrawal_of_oral_artemisinin_based_monotherapies/en/). 2017
.5.
World Health Organization (WHO)
. Assessment and monitoring of antimalarial drug efficacy for the treatment of uncomplicated falciparum malaria
. Geneva
; 2003
.6.
Talisuna
AO
, Bloland
P
, D’Alessandro
U
. History, dynamics, and public health importance of malaria parasite resistance
. Clin Microbiol Rev
. 2004
Jan
;17
(1
):235
–54
.
[PubMed]
0893-85127.
Wondimu
T
, Asfaw
Z
, Kelbessa
E
. Ethnobotanical study of medicinal plants around ‘Dheeraa’ town, Arsi Zone, Ethiopia
. J Ethnopharmacol
. 2007
May
;112
(1
):152
–61
.
[PubMed]
0378-87418.
Kayne
SB
. Homeopathic pharmacy: Theory and practice
. Edinburgh (NY)
: Elsevier Churchill Livingstone
; 2006
. p. 386
.9.
Sharma
A
, Purkait
B
. Identification of medicinally active ingredient in ultra diluted Digitalis purpurea: fluorescence spectroscopic and cyclic-voltammetric study
. J Anal Methods Chem
. 2012
;2012
(Mar
):109058
.
[PubMed]
2090-887310.
Dubey
SK
. Text book of materia medica
[including Allen’s keynote- an easy explanation
]New Delhi
: Books and Allied
; 2007
. pp. 1
–184
.11.
Government of India, Homeopathic Pharmacopoeia of India Vol.-I.
1971
.12.
13.
Trager
W
, Jensen
JB
. Human malaria parasites in continuous culture
. Science
. 1976
Aug
;193
(4254
):673
–5
.
[PubMed]
0036-807514.
World Health Organisation (WHO)
. In vitro Micro Test (Mark III) for the assessment of response of Plasmodium falciparum to chloroquine, melfoquine, quinine, amodiaquine, sulfadoxine pyrimethamine and artemisinin. Geneva. CTD/MAL/ 97, 20, 2001
.15.
Mosmann
T
. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays
. J Immunol Methods
. 1983
Dec
;65
(1-2
):55
–63
.
[PubMed]
0022-175916.
Knight
DJ
, Peters
W
. The antimalarial activity of N-benzyloxydihydrotriazines. I. The activity of clociguanil (BRL 50216) against rodent malaria, and studies on its mode of action
. Ann Trop Med Parasitol
. 1980
Aug
;74
(4
):393
–404
.
[PubMed]
0003-498317.
Peters
W
. Drug resistance in Plasmodium berghei Vincke and Lips, 1948. I. Chloroquine resistance
. Exp Parasitol
. 1965
Aug
;17
(1
):80
–9
.
[PubMed]
0014-489418.
Ryley
JF
, Peters
W
. The antimalarial activity of some quinolone esters
. Ann Trop Med Parasitol
. 1970
Jun
;64
(2
):209
–22
.
[PubMed]
0003-498319.
World Health Organization (WHO)
. Antimalarial drug resistance in the Greater Mekong Subregion: How concerned should we be? https://www.who.int/malaria/media/drug-resistance-greater-mekong-qa/en/. 2017
.20.
Aleixo
DL
, Ferraz
FN
, Ferreira
ÉC
, de Lana
M
, Gomes
ML
, de Abreu Filho
BA
, et al Highly diluted medication reduces parasitemia and improves experimental infection evolution by Trypanosoma cruzi
. BMC Res Notes
. 2012
Jul
;5
(1
):352
.
[PubMed]
1756-050021.
Bellavite
P
, Ortolani
R
, Pontarollo
F
, Pitari
G
, Conforti
A
. Immunology and homeopathy. 5. The rationale of the ‘Simile’
. Evid Based Complement Alternat Med
. 2007
Jun
;4
(2
):149
–63
.
[PubMed]
1741-427X22.
Lekana-Douki
JB
, Bongui
JB
, Oyegue Liabagui
SL
, Zang Edou
SE
, Zatra
R
, Bisvigou
U
, et al In vitro antiplasmodial activity and cytotoxicity of nine plants traditionally used in Gabon
. J Ethnopharmacol
. 2011
Feb
;133
(3
):1103
–8
.
[PubMed]
1872-757323.
Blair
S
, Mesa
J
, Correa
A
, Carmona-Fonseca
J
, Granados
H
, Sáez
J
. Antimalarial activity of neurolenin B and derivates of Eupatorium inulaefolium (Asteraceae)
. Pharmazie
. 2002
Jun
;57
(6
):413
–5
.
[PubMed]
0031-714424.
Carvalho
LH
, Brandão
MG
, Santos-Filho
D
, Lopes
JL
, Krettli
AU
. Antimalarial activity of crude extracts from Brazilian plants studied in vivo in Plasmodium berghei-infected mice and in vitro against Plasmodium falciparum in culture
. Braz J Med Biol Res
. 1991
;24
(11
):1113
–23
.
[PubMed]
0100-879X25.
Bagai
U
, Rajan
A
, Kaur
S
. Antimalarial potential of Nosode 30 and 200 against Plasmodium berghei infection in BALB/c mice
. J Vector Borne Dis
. 2012
Jun
;49
(2
):72
–7
.
[PubMed]
0972-906226.
Malomo
SO
. Toxicological implication of ceftriaxone administration in rats
. NSBMB
. 2000
;15
:33
–8
.27.
Gorki
V
, Singh
R
, Walter
NS
, Bagai
U
, Salunke
DB
. Synthesis and evaluation of antiplasmodial efficacy of β-Carboline derivatives against murine malaria
. ACS Omega
. 2018
Oct
;3
(10
):13200
–10
.
[PubMed]
2470-134328.
Gorki
V
, Walter
NS
, Singh
R
, Chauhan
M
, Dhingra
N
, Salunke
DB
, et al β-Carboline derivatives tackling malaria: biological evaluation and docking analysis
. ACS Omega
. 2020
Jul
;5
(29
):17993
–8006
.
[PubMed]
2470-134329.
Nyblom
H
, Björnsson
E
, Simrén
M
, Aldenborg
F
, Almer
S
, Olsson
R
. The AST/ALT ratio as an indicator of cirrhosis in patients with PBC
. Liver Int
. 2006
Sep
;26
(7
):840
–5
.
[PubMed]
1478-322330.
Mouton
R
, Holder
K
. Laboratory tests of renal function
. Anaesth Intensive Care Med
. 2006
Jul
;7
(7
):240
–3
. 1472-029931.
Musabayane
CT
, Ndhlovu
CE
, Balment
RJ
. The effects of oral chloroquine administration on kidney function
. Ren Fail
. 1994
;16
(2
):221
–8
.
[PubMed]
0886-022X32.
Rajan
A
, Bagai
U
. Antimalarial potential of China 30 and Chelidonium 30 in combination therapy against lethal rodent malaria parasite: plasmodium berghei
. J Complement Integr Med
. 2013
May
;10
(1
):89
–96
.
[PubMed]
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