Aims: This study aimed to evaluate the different abilities of various Phellinus igniarius strains inhibiting quorum sensing (QS), to search for novel QS inhibitors from them and to analyze their inhibitory activity, with a view to their possible use in controlling infections. Methods: The bioactive metabolites produced by P. igniarius cultures were tested for their abilities to inhibit QS-regulated behavior. All P. igniarius strains were cultured in potato-dextrose medium by large-scale submerged fermentation. The culture supernatant was condensed into 0.2-fold volumes by freeze drying. The condensed supernatant was sterilized by filtration through a 0.22-µm membrane filter and added to Chromobacterium violaceum CV026 cultures, which were used to monitor QS inhibition. Inhibitory activity was measured by quantifying violacein production using a microplate reader. Results: The bioactive metabolites produced by 10 P. igniarius strains could inhibit violacein production, a QS-regulated behavior in C. violaceum. Furthermore, these strains could be roughly categorized into three groups on the basis of their inhibitory activities. Conclusions:P. igniarius strains can produce QS-inhibitory compounds and have different abilities to inhibit QS.

Keywords:
Bioactive metabolites, Chromobacterium violaceum CV026, N-Hexanoyl-L-homoserine lactone, Anti-QS, Phellinus igniarius
1.
Miller MB, Bassler BL: Quorum sensing in bacteria. Annu Rev Microbiol 2001;55:165–199.
2.
Choudhary S, Schmidt-Dannert C: Applications of quorum sensing in biotechnology. Appl Microbiol Biotechnol 2010;86:1267–1279.
3.
Antunes LC, Ferreira RB, Buckner MM, Finlay BB: Quorum sensing in bacterial virulence. Microbiology 2010;156:2271–2282.
4.
Dickschat JS: Quorum sensing and bacterial biofilms. Nat Prod Rep 2010;27:343–369.
5.
Fineran PC, Slater H, Everson L, Hughes K, Salmond GP: Biosynthesis of tripyrrole and β-lactam secondary metabolites in Serratia: integration of quorum sensing with multiple new regulatory components in the control of prodigiosin and carbapenem antibiotic production. Mol Microbiol 2005;56:1495–1517.
6.
Kim MY, Park RY, Choi MH, Sun HY, Kim CM, Kim SY, Rhee JH, Shin SH: Swarming differentiation of Vibrio vulnificus downregulates the expression of the vvhBA hemolysin gene via the LuxS quorum-sensing system. J Microbiol 2006;44:226–232.
7.
Morohoshi T, Kato M, Fukamachi K, Kato N, Ikeda T: N-acylhomoserine lactone regulates violacein production in Chromobacterium violaceum type strain ATCC 12472. FEMS Microbiol Lett 2008;279:124–130.
8.
Slater H, Crow M, Everson L, Salmond GP: Phosphate availability regulates biosynthesis of two antibiotics, prodigiosin and carbapenem, in Serratia via both quorum-sensing-dependent and -independent pathways. Mol Microbiol 2003;47:303–320.
9.
von Bodman SB, Majerczak DR, Coplin DL: A negative regulator mediates quorum-sensing control of exopolysaccharide production in Pantoea stewartii subsp. stewartii. Proc Natl Acad Sci USA 1998;95:7687–7692.
10.
White CE, Winans SC: Cell-cell communication in the plant pathogen Agrobacterium tumefaciens. Phil Trans R Soc Lond B Biol Sci 2007;362:1135–1148.
11.
Rasmussen TB, Givskov M: Quorum-sensing inhibitors as anti-pathogenic drugs. Int J Med Microbiol 2006;296:149–161.
12.
Manefield M, de Nys R, Kumar N, Read R, Givskov M, Steinberg P, Kjelleberg S: Evidence that halogenated furanones from Delisea pulchra inhibit acylated homoserine lactone (AHL)-mediated gene expression by displacing the AHL signal from its receptor protein. Microbiology 1999;145:283–291.
13.
Zhu H, Shen YL, Wei DZ, Zhu JW: Inhibition of quorum sensing in Serratia marcescens H30 by molecular regulation. Curr Microbiol 2008;56:645–650.
14.
Dong YH, Xu JL, Li XZ, Zhang LH: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci USA 2000;97:3526–3531.
15.
Amara N, Krom BP, Kaufmann GF, Meijler MM: Macromolecular inhibition of quorum sensing: enzymes, antibodies, and beyond. Chem Rev 2011;111:195–208.
16.
Musthafa KS, Ravi AV, Annapoorani A, Packiavathy IS, Pandian SK: Evaluation of anti-quorum-sensing activity of edible plants and fruits through inhibition of the N-acyl-homoserine lactone system in Chromobacterium violaceum and Pseudomonas aeruginosa. Chemotherapy 2010;56:333–339.
17.
Singh BN, Singh HB, Singh A, Singh BR, Mishra A, Nautiyal CS: Lagerstroemia speciosa fruit extract modulates quorum sensing-controlled virulence factor production and biofilm formation in Pseudomonas aeruginosa. Microbiology 2012;158:529–538.
18.
Zhu H, Wang SX, Zhang SS, Cao CX: Inhibiting effect of bioactive metabolites produced by mushroom cultivation on bacterial quorum sensing-regulated behaviors. Chemotherapy 2011;57:292–297.
19.
Moradali MF, Mostafavi H, Hejaroude GA, Tehrani AS, Abbasi M, Ghods S: Investigation of potential antibacterial properties of methanol extracts from fungus Ganoderma applanatum. Chemotherapy 2006;52:241–244.
20.
Zhu H, Liu W, Tian B, Liu H, Ning S: Inhibition of quorum sensing in the opportunistic pathogenic bacterium Chromobacterium violaceum by an extract from fruiting bodies of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (higher Basidiomycetes). Int J Med Mushrooms 2011;13:559–564.
21.
Zhu H, Sun S, Zhang S: Enhanced production of total flavones and exopolysaccharides via Vitreoscilla hemoglobin biosynthesis in Phellinus igniarius. Bioresour Technol 2011;102:1747–1751.
22.
Zhu H, He CC, Chu QH: Inhibition of quorum sensing in Chromobacterium violaceum by pigments extracted from Auricularia auricular. Lett Appl Microbiol 2011;52:269–274.
23.
Zhu H, Sun SJ: Inhibition of bacterial quorum sensing-regulated behaviors by Tremella fuciformis extract. Curr Microbiol 2008;57:418–422.
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2012
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