A microsatellite locus from the Neotropical fish genus Prochilodus was isolated using PCR-based isolation of microsatellite arrays. Of 470 positive clones, 15 were sequenced, and 10 of them showed an (AATTT)n repeat. Primers were designed, and analysis of polymorphism revealed 11 alleles in three Prochilodus species. Fluorescence in situ hybridization analysis showed signals predominantly in the telomeric regions of several chromosomes. The description of this microsatellite may contribute to studies of the population structure of this fish group.   

1.
Bertollo LAC, Takahashi CS, Moreira-Filho O: Cytotaxonomic consideration on Hoplias lacerdae (Pisces, Erythrinidae). Rev bras Genet 1:103–120 (1978).
2.
Calcagnotto D, Russello M, DeSalle R: Isolation and characterization of microsatellite loci in Piaractus mesopotamicus and their applicability in other Serrasalminae fish. Mol Ecol Notes 1:245–247 (2001).
3.
Castro RMC: Revisão taxonômica da família Prochilodontidae (Ostriophysi, Characiformes). PhD thesis, Universidade de São Paulo, Ribeirão Preto, Brazil (1990).
4.
Comincini S, Leone P, Redaelli L, De Giuli L, Zhang Y, Ferreti L: Characterization of bovine microsatellites by silver staining. J anim Breed Genet 112:415–420 (1995).
5.
Cross S, Lindsey J, Fantes J, McKay S, McGill N, Cooke H: The structure of a subterminal repeated sequence present on many human chromosomes. Nucl Acids Res 18:6649–6657 (1990).
6.
Flecker AS: Ecosystem engineering by a dominant detritivore in a diverse tropical stream. Ecology 77:1845–1854 (1996).
7.
Lunt DH, Hutchinson WF, Carvalho GR: An efficient method for PCR-based isolation of microsatellite arrays (PIMA). Mol Ecol 8:891–894 (1999).
8.
Meyne J, Ratliff RL, Moyzis RK: Conservation of the human telomere sequence (TTAGGG)n among vertebrates. Proc natl Acad Sci, USA 86:7049–7053 (1989).
9.
Morishima K, Nakayama I, Arai K: Microsatellite-centromere mapping in the loach, Misgurnus anguillicaudatus. Genetica 111:59–69 (2001).
10.
Pinkel D, Straume T, Gray JW: Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938 (1986).
11.
Rosenberg M, Hui L, Ma J, Nusbaum HC, Clark K, Robinson L, Dziadzio L, Swain PM, Keith T, Hudson TJ, Biesecker LG, Flint J: Characterization of short tandem repeats from thirty-one humam telomeres. Genome Res 7:917–923 (1997).
12.
Sakamoto T, Danzmann RG, Gharbi K, Howard P, Ozaki A, Khoo SK, Woram RA, Okamoto N, Ferguson MM, Holm LE, Guyomard R, Hoyheim B: A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates. Genetics 155:1331–1345 (2000).
13.
Sambrook J, Fritish EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd Ed (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989).
14.
Shimoda N, Knapik EW, Ziniti J, Sim C, Yamada E, Kaplan S, Jackson D, Sauvage F, Jacob H, Fishman MC: Zebrafish genetic map with 2000 microsatellite markers. Genomics 58:219–232 (1999).
© 2003 S. Karger AG, Basel
2003
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.