Peripheral blood cell cultures were treated for late incorporation of both BrdU and Hoechst-33258 to obtain R-banding pattern preparations. Twenty-eight bovine cosmids from 19 bovine syntenic groups (U), three of which contain type I loci and 25 which contain microsatellite loci and have previously been assigned to cattle chromosomes, were comparatively FISH-mapped to sheep and river buffalo chromosomes according to the standard karyotypes (13 loci for the first time in the latter species). The results enrich the physical maps of both species with information relative to the following loci and to the corresponding syntenic groups: IDVGA35 and IDVGA53 (U6), IDVGA61 and IDVGA84 (U13), JAB10 (U5), IDVGA41 and IDVGA57 (U27), IDVGA87 (U11), IDVGA32 and IDVGA10 (U19), IDVGA49, IDVGA66 and IDVGA68 (U1), ZNF164 (U23), IDVGA74 and IDVGA70 (U9), IDVGA47, IDVGA46 and IDVGA58 (U21), MAP1B (U14), IDVGA79 (U4), CATHL (U12), IDVGA71 (U8), IDVGA59 (U26), IDVGA29 (U29), IDVGA7 (U7), IDVGA82 (X), IDVGA50 (Y). All mapped loci were localized on homoeologous chromosomes and chromosome regions of the two species, confirming the high degree of chromosome homoeologies between the subfamilies Bovinae and Caprinae.    

1.
CSKBB: Standard karyotype of the river buffalo (Bubalus bubalis L., 2n = 50). Report of the committee for the standardization of banded karyotypes of the river buffalo (L. Iannuzzi, co-ordinator). Cytogenet Cell Genet 67:102–113 (1994).
2.
Di Meo GP, Perucatti A, Iannuzzi L, Rangel-Figueiredo MT, Ferrara L: Constitutive heterochromatin polymorphism in river buffalo chromosomes. Caryologia 48:137–145 (1995).
3.
Di Meo GP, Perucatti A, Schibler L, Incarnato D, Ferrara L, Cribiu EP, Iannuzzi L: Thirteen type I loci from HSA4q, HSA6p, HSA7q and HSA12q were comparatively FISH-mapped in four river buffalo and sheep chromosomes. Cytogenet Cell Genet 90:102–105 (2000).
4.
Ferretti L, Urquhart BGD, Eggen A, Olsaker I, Harlizius B, Castiglioni B, Mezzelani A, Solinas Toldo S, Thieven U, Zhang Y, Morgan ALG, Teres VM, Schwerin M, Martin-Burriel I, Chowdhary BP, Erhardt G, Nijman IJ, Cribiu EP, Barendse W, Leveziel H, Fries R, Williams JL: Cosmid-derived markers anchoring the bovine genetic map to the physical map. Mammal Genome 8:29–36 (1997).
5.
Gallagher DS Jr, Womack JE: Chromosome conservation in the Bovidae. J Hered 83:287–298 (1992).
6.
Hayes H, Petit E, Dutrillaux B: Comparison of RBG-banded karyotypes of cattle, sheep, and goats. Cytogenet Cell Genet 57:51–55 (1991).
7.
Iannuzzi L: A genetic physical map in river buffalo (Bubalus bubalis, 2n = 50). Caryologia 51:311–318 (1998).
8.
Iannuzzi L, Di Meo GP: Chromosomal evolution in bovids: a comparison of cattle, sheep and goat G- and R-banded chromosomes and cytogenetic divergences among cattle, goat and river buffalo sex chromosomes. Chrom Res 3:291–299 (1995).
9.
Iannuzzi L, Gallagher DS, Di Meo GP, Yang Y, Womack JE, Davis SK, Taylor JF: Comparative FISH-mapping of six expressed gene loci to river buffalo and sheep. Cytogenet Cell Genet 84:161–163 (1999).
10.
Iannuzzi L, Di Meo GP, Perucatti A, Incarnato D, Schibler L, Cribiu EP: Comparative FISH-mapping of bovid X chromosomes reveals homologies and divergences between the subfamilies Bovinae and Caprinae. Cytogenet Cell Genet 89:171–176 (2000a).
11.
Iannuzzi L, Di Meo GP, Perucatti A, Schibler L, Incarnato D, Ferrara L, Bardaro T, Cribiu EP: Sixteen type I loci from six chromosomes were comparatively fluorescent in-situ mapped to river buffalo (Bubalus bubalis) and sheep (Ovis aries) chromosomes. Chrom Res 8:447–450 (2000b).
12.
Iannuzzi L, Di Meo GP, Perucatti A, Schibler L, Incarnato D, Cribiu EP: Comparative FISH-mapping in river buffalo and sheep chromosomes: assignment of forty autosomal type I loci from sixteen human chromosomes. Cytogenet Cell Genet 94:43–48 (2001a).
13.
Iannuzzi L, Molteni L, Di Meo GP, De Giovanni A, Perucatti A, Succi G, Incarnato D, Eggen A, Cribiu EP: A case of azoospermia in a bull carrying a Y-autosome reciprocal translocation. Cytogenet Cell Genet 95:225–227 (2001b).
14.
Iannuzzi L, Di Meo GP, Perucatti A, Eggen A, Incarnato D, Sarubbi F, Cribiu E: A pericentric inversion in cattle Y-chromosome. Cytogenet Cell Genet 94:202–205 (2001c).
15.
ISCNDB (2000): International system for chromosome nomenclature of domestic bovids, Di Berardino D, Di Meo GP, Gallagher DS, Hayes H, Iannuzzi L (co-ordinator) (eds) Cytogenet Cell Genet 92:283–299 (2001).
16.
Mezzelani A, Zhang Y, Redaelli L, Castiglioni B, Leone P, Williams J, Solinas-Toldo S, Wigger G, Fries R, Ferretti L: Chromosomal localization and molecular characterization of 53 cosmid-derived bovine microsatellites. Mammal Genome 6:629–635 (1995).
17.
Piumi F, Schibler L, Vaiman D, Oustry A., Cribiu EP: Comparative cytogenetic mapping reveals chromosome rearrangements between the X chromosomes of two closely related mammalian species (cattle and goats). Cytogenet Cell Genet 81:36–41 (1998).
18.
SheepBase: http://angis.thearkdb.org/anubis (October 2002).
© 2003 S. Karger AG, Basel
2003
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.