In Acricotopus lucidus (Diptera, Chironomidae) the germ line-limited chromosomes (Ks) have developed from the soma chromosomes (Ss) by endoreduplication, rearrangements and accumulation of germ line-specific repetitive sequences. For molecular analysis of specific small K sections, microdissection of metaphase Ks generally yields very limited amounts of DNA. In this study, K-specific DNA was microdissected from defined polytenized K sections of X-ray induced K-S-rearrangements of permanent salivary gland chromosome preparations and was then amplified by DOP-PCR. A new germ line-specific tandem repetitive DNA family was isolated by this way from a heterochromatic K segment, characterized and localized on the Ks by FISH. The repetitive elements are related to sequences of earlier described K-specific tandem repetitive DNA families in A. lucidus, but are located mainly in terminal heterochromatin bands of the two largest Ks and only to a limited degree in the paracentromeric K heterochromatin. This demonstrates that a collection of permanent preparations of K-S-rearrangements with polytenized heterochromatic and S-homologous K sections of A. lucidus can be used as a source for obtaining K sequences of defined K parts to investigate the molecular evolution of the Ks.   

Bauer H: Rearrangements between germ-line limited and somatic chromosomes in Smittia parthenogenetica (Chironomidae, Diptera). Chromosoma 32: 1–10 (1970).
Bauer H, Beermann W: Der Chromosomencyclus der Orthocladiinen (Nematocera, Diptera). Z Naturforsch 7b:557–563 (1952).
Bell KA, Van Deerlin PG, Feinberg RF, du Manoir S, Haddad BR: Diagnosis of aneupoidy in archival, paraffin-embedded pregnancy-loss tissues by comparative genomic hybridization. Fert Steril 75:374–379 (2001).
Carter NP: Cytogenetic analysis by chromosome painting. Cytometry 18:2–10 (1994).
Elder JF, Turner BJ: Concerted evolution of repetitive DNA sequences in eukaryotes. Q Rev Biol 70:297–320 (1995).
Goday C, Esteban MR: Chromosome elimination in sciarid flies. BioEssays 23:242–250 (2001).
Goto Y, Kubota S, Kohno S: Highly repetitive DNA sequences that are restricted to the germ line in the hagfish Eptatretus cirrhatus: a mosaic of eliminated elements. Chromosoma 107:17–32 (1998).
Kohno S, Nakai Y, Satoh S, Yoshida M, Kobayashi H: Chromosome elimination in Japanese hagfish, Eptatretus burgeri (Agnatha, Cyclostomata). Cytogenet Cell Genet 41:209–214 (1986).
Kubota S, Kuro-o M, Mizuno S, Kohno S: Germ line-restricted, highly repeated DNA sequences and their chromosomal localization in a Japanese hagfish (Eptatretus okinoseanus). Chromosoma 102:163–173 (1993).
Li W-H: Concerted evolution of multigene families, in: Molecular Evolution, pp 309–334 (Sinauer Associates, Sunderland 1997).
Marchio A, Terris B, Meddeb M, Pineau P, Duverger A, Tiollais P, Bernheim A, Dejean A: Chromosomal abnormalities in liver cell dysplasia detected by comparative genomic hybridisation. Mol Pathol 54:270–274 (2001).
Müller S, Neusser M, Wienberg J: Towards unlimited colors for fluorescence in-situ hybridization (FISH). Chrom Res 10:223–232 (2002).
Redi AA, Garagna S, Zacharias H, Zuccotti M, Capanna E: The other chromatin. Chromosoma 110:136–147 (2001).
Sharma AK, Sharma A: Chromosome painting – principles, strategies and scope. Methods Cell Sci 23:1–5 (2001).
Staiber W: Unusual germ line limited chromosomes in Acricotopus lucidus (Diptera, Chironomidae). Genome 29:702–705 (1987).
Staiber W: G-banding of germ line limited chromosomes in Acricotopus lucidus (Diptera, Chironomidae). Chromosoma 97:231–234 (1988).
Staiber W: Structural homologies between germ line limited and soma chromosomes in Acricotopus lucidus (Diptera, Chironomidae). J Hered 82:247–249 (1991).
Staiber W, Behnke E: Developmental puffing activity in the salivary and Malpighian tubule chromosomes of Acricotopus lucidus (Diptera, Chironomidae). Chromosoma 93:1–16 (1985).
Staiber W, Thudium D: X-ray induced rearrangements between germ-line limited and soma chromosomes of Acricotopus lucidus (Diptera, Chironomidae). Genetica 69:149–156 (1986).
Staiber W, Schiffkowski C: Structural evolution of the germ line-limited chromosomes in Acricotopus. Chromosoma 109:343–349 (2000).
Staiber W, Wahl S: Painting analysis of meiotic metaphase I configurations of the germ line-limited chromosomes in Acricotopus. Chrom Res 10:101–108 (2002).
Staiber W, Wech I., Preiss: A Isolation and localization of a germ line-specific highly repetitive DNA family in Acricotopus lucidus (Diptera, Chironomidae). Chromosoma 106:267–275 (1997).
Teixeira MR: Combined classical and molecular cytogenetic analysis of cancer. Eur J Cancer 38:1580–1584 (2002).
Telenius H, Carter NP, Bepp CE, Nordenskjöld M, Ponder BA, Tunnacliffe A: Degenerate oligonucleotide-primed PCR: general amplification of target DNA by a single degenerate primer. Genomics 13:718–725 (1992).
Tobler H: The differentiation of germ and somatic cell lines in nematodes, in Hennig W (ed): Germ Line-soma Differentiation, Results and Problems in Cell Differentiation, Vol 13, pp 1–69 (Springer-Verlag, Berlin 1986).
Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acids Res 22:4673–4680. (1994).
Verhagen PC, Zhu XL, Rohr LR, Cannon-Albright LA, Tavtigian SV, Skolnick MH, Brothman AR: Microdissection, DOP-PCR, and comparative hybridization of paraffin-embedded familial prostate cancers. Cancer Genet Cytogenet 122:43–48 (2000).
Walker PMB: “Repetitive” DNA in higher organisms, in Butler JAV, Noble D (eds): Prog Biophys Mol Biol, Vol 23, pp 147–190 (Pergamon, Oxford 1971).
White MJD: Animal Cytology and Evolution, 3rd ed, pp 500–546 (Cambridge University Press, Cambridge 1973).
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