Image restoration approaches, such as digital deconvolution, are becoming widely used for improving the quality of microscopic images. However, no quantification of the gain in resolution of fluorescence images is available. We show that, after iterative constrained deconvolution, fluorescent cosmid signals appear to be 25% smaller, and 1.2-kb fragment signals on combed molecules faithfully display the expected length.   

Bensimon A, Simon A, Chiffaudel A, Croquette V, Heslot F, Bensimon D: Alignment and sensitive detection of DNA by a moving interface. Science 265:2096–2098 (1994).
Borsani G, Tonlorenzi R, Simmler M, Dandolo L, Arnaud D, Capra V, Grompe M, Pizzuti A, Muzny D, Lawrence C, et al: Characterization of a murine gene expressed from the inactive X chromosome. Nature 351:325–329 (1991).
Jolly C, Mongelard F, Robert-Nicoud M, Vourc’h C: Optimization of nuclear transcripts detection by FISH and combination with fluorescence immunocytochemical detection of transcription factors. Histochem Cytochem 45:1585–1592 (1997).
Monier K, Hazzouri M, Ekong R, Rousseaux S: Optimize FISH on combed DNA molecules. Technical Tips Online ( T01417 (1998a).
Monier K, Michalet X, Lamartine J, Schurra C, Heitzmann F, Yin L, Cinti R, Sylla BS, Creaven M, Porta G, Vourc’h C, Robert-Nicoud M, Bensimon A, Romeo G: High-resolution mapping of the X-linked lymphoproliferative syndrome region by FISH on combed DNA. Cytogenet Cell Genet 81:259–264 (1998b).
Monier K, Usson Y, Mongelard F, Szepetowsky P, Robert-Nicoud M, Vourc’h C: Metaphase and interphase mapping by FISH: improvement of chromosome banding and signals resolution by means of iterative deconvolution. Cytogenet Cell Genet 72:200–204 (1996).
Raynaud SD, Bekri S, Leroux D, Grosgeorge J, Klein B, Bastard C, Gaudray P, Simon MP: Expanded range of 11q13 breakpoints with differing patterns of cyclin-d1 expression in B-cell malignancies. Genes Chrom Cancer 8:80–87 (1993).
Simmler M, Cunningham D, Clerc P, Vermat T, Caudron B, Cruaud C, Pawlak A, Szpirer C, Weissenbach J, Clavelie J, Avner P: A 94 kb genomic sequence 3′ to the murine Xist gene reveals an AT rich region containing a new testis specific gene, Tsx. Hum molec Genet 5:1713–1726 (1996).
Weier HUG, Wang M, Mullikin JC, Zhu Y, Cheng JF, Greulich KM, Bensimon A, Gray JW: Quantitative DNA fiber mapping. Hum molec Genet 4:1903–1910 (1995).
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.