Recently, the EXTL1 gene, a member of the EXT tumor suppressor gene family, has been mapped to 1p36, a chromosome region which is frequently implicated in a wide variety of malignancies, including breast carcinoma, colorectal cancer and neuroblastoma. In this study, we show that the EXTL1 gene is located between the genetic markers D1S511 and D1S234 within 200 kb of the LAP18 gene on chromosome 1p36.1, a region which has been proposed to harbor a tumor suppressor gene implicated in MYCN-amplified neuroblastomas. In addition, we determined the genomic structure of the EXTL1 gene, revealing that the EXTL1 coding sequence spans 11 exons within a 50-kb region.

1.
Ahn J, Lüdecke H, Lindow S, Horton WA, Lee B, Wagner MJ, Horsthemke B, Wells DE: Cloning of the putative tumour suppressor gene for hereditary multiple exostoses (EXT1). Nature Genet 11:137–143 (1995).
2.
Beilaiche Y, The I, Perrimon N: Tout-velu is a Drosophila homologue of the putative tumour suppressor EXT-1 and is needed for Hh diffusion. Nature 394:85–88 (1998).
3.
Caron H, Peter M, Van Sluis P, Speleman F, De Kraker J, Laureys G, Michon J, Brugieres L, Voute PA, Westerveld A, Slater R, Delattre O, Versteeg R: Evidence for two tumour suppressor loci on chromosomal bands 1p35→p36 involved in neuroblastoma: one probably imprinted, another associated with N-myc amplification. Hum Mol Genet 4:535–539 (1995).
4.
Cheng NC, Van Roy N, Chan A, Beitsma M, Westerveld A, Speleman F, Versteeg R: Deletion mapping in neuroblastoma cell lines suggests two distinct tumor suppressor genes in the 1p35→p36 region, only one of which is associated with N-myc amplification. Oncogene 10:291–297 (1995).
5.
Lehrach H: Genetic and physical mapping, in Davies KE, Tilghman SM (eds): Genome analysis, pp 39–81 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1990).
6.
Lind T, Tufaro F, McCormick C, Lindahl U, Lidholt K: The putative tumor suppressors EXT1 and EXT2 are glycosyltranserases required for the biosynthesis of heparan sulfate. J biol Chem 273:26265–26268 (1998).
7.
McCormick C, Leduc Y, Martindale D, Mattison K, Esford LE, Dyer AP, Tufaro F: The putative tumour suppressor EXT1 alters the expression of cell-surface heparan sulfate. Nature Genet 19:158–161 (1998).
8.
Melhem RF, Zhu XX, Hailat N, Strahler JR, Hanash SM: Characterization of the gene for a proliferation-related phosphoprotein (oncoprotein 18) expressed in high amounts in acute leukemia. J biol Chem 266:17747–17753 (1991).
9.
Schwab M, Praml C, Amler LC: Genomic instability in 1p and human malignancies. Genes Chrom Cancer 16:211–229 (1996).
10.
Solomon L: Bone growth in diaphyseal aclasis. J Bone Joint Surg 43:700–716 (1961).
11.
Stickens D, Clines G, Burbee D, Ramos P, Thomas S, Hogue D, Hecht JT, Lovett M, Evans GA: The EXT2 multiple exostoses gene defines a family of putative tumour suppressor genes. Nature Genet 14:25–32 (1996).
12.
Van Hul W, Wuyts W, Hendrickx J, Speleman F, Wauters J, De Boulle K, Van Roy N, Bossuyt P, Willems PJ: Identification of a third EXT-like gene (EXTL3) belonging to the EXT gene family. Genomics 47:230–237 (1998).
13.
Van Roy N, Cheng NC, Laureys G, Opdenakker G, Versteeg R, Speleman F: Molecular cytogenetic analysis of 1;17 translocations in neuroblastoma. Eur J Cancer 31A:530–534 (1995).
14.
Van Roy N, Laureys G, Ching Cheng N, Willem P, Opdenakker G, Versteeg R, Speleman F: 1;17 translocations and other chromosome 17 rearrangements in human primary neuroblastoma tumor and cell lines. Genes Chrom Cancer 10:103–114 (1994).
15.
Van Roy N, Laureys G, Versteeg R, Opdenakker G, Speleman F: High resolution fluoresence mapping of 46 DNA markers to the short arm of human chromosome 1. Genomics 18:71–78 (1993).
16.
Versteeg R, Caron H, Cheng NC, van der Drift P, Slater R, Westerveld A, Voute PA, Delattre O, Laureys G, Van Roy N, Speleman F: 1p36: every subband a suppressor? Eur J Cancer 31A:538–540 (1995).
17.
Wicklund CL, Pauli RM, Johnston D, Hecht JT: Natural history study of hereditary multiple exostoses. Am J med Genet 55:43–46 (1995).
18.
Wise CA, Clines GA, Massa H, Trask BJ, Lovett M: Identification and localization of the gene for EXTL, a third member of the multiple exostoses gene family. Genome Res 7:10–16 (1997).
19.
Wuyts W, Van Hul W, Hendrickx J, Wauters J, Speleman F, De Boulle K, Bossuyt P, Van Agtmael T, Willems PJ: Identification and characterization of a novel member of the EXT gene family, EXTL2. Eur J hum Genet 5:382–389 (1997).
20.
Wuyts W, Van Hul W, Wauters J, Nemtsova M, Reyniers E, Van Hul E, De Boulle K, de Vries BBA, Hendrickx J, Herrygers I, Bossuyt P, Balemans W, Fransen E, Vits L, Coucke P, Nowak NJ, Shows TB, Mallet L, van den Ouweland AMW, McGaughran J, Halley DJJ, Willems PJ: Positional cloning of a gene involved in hereditary multiple exostoses. Hum Mol Genet 5:1547–1557 (1996).
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