The product of the spermatid-specific rat SPAG4 gene interacts specifically with the major 27-kDa outer dense fiber protein ODF27 in elongating spermatids. In this study we isolated the human homolog of the spermatid-specific rat SPAG4 gene and mapped it by fluorescence in situ hybridization to chromosome 20q11.2.

1.
Burfeind R, Hoyer-Fender S: Sequence and developmental expression of an mRNA encoding a putative protein of rat sperm outer dense fibers. Devl Biol 148:195–204 (1991).
2.
Demetrick DJ: Fluorescence in situ hybridization and human cell cycle genes, in Pagano M (ed): The Cell Cycle—Materials and Methods, pp 29–45 (Springer Verlag, Berlin/Heidelberg/New York 1995).
3.
Fawcett DW: A comparative view of sperm ultrastructure. Biol Reprod 2(Suppl):90–127 (1970).
4.
Gastmann O, Burfeind P, Gunther E, Hameister H, Szpirer C, Hoyer-Fender S: Sequence, expression, and chromosomal assignment of a human sperm outer dense fiber gene. Mol Reprod Dev 36:407–418 (1993).
5.
Morales CR, Oko R, Clermont Y: Molecular cloning and developmental expression of an mRNA encoding the 27 kDa outer dense fiber protein of rat spermatozoa. Mol Reprod Dev 37:229–240 (1994).
6.
Oko R: Comparative analysis of proteins from the fibrous sheath and outer dense fibers of rat spermatozoa. Biol Reprod 39:169–182 (1988).
7.
Oko R. Clermont Y: Light microscopic immunocytochemical study of fibrous sheath and outer dense fiber formation in the rat spermatid. Anat Rec 225:46–55 (1989).
8.
Olson GE, Sammons DW: Structural chemistry of outer dense fibers of rat sperm flagellum. Biol Reprod 22:319–332 (1980).
9.
Phillips DM: Comparative analysis of mammalian sperm motility. J Cell Biol 53:561–573 (1972).
10.
Shao X, van der Hoorn FA: Self-interaction of the major 27-kilodalton outer dense fiber protein is in part mediated by a leucine zipper domain in the rat. Biol Reprod 55:1343–1350 (1996).
11.
Shao X, Tarnasky HA, Schalles U, Oko R, van der Hoorn FA: Interactional cloning of the 84-kDa major outer dense fiber protein ODF84: leucine zippers mediate associations of ODF84 and ODF27. J biol Chem 272:6105–6113 (1997).
12.
van der Hoorn FA, Tarnasky HA, Nordeen SK: A new rat gene RT7 is specifically expressed during spermatogenesis. Dev Biol 142:147–154 (1990).
13.
Vera JC, Brito M, Zuvic T, Burzio LO: Polypeptide composition of rat sperm outer dense fibers. J Biol Chem 259:5970–5977 (1984).
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