Human chromosome-specific probes for the entire karyotype were hybridized to metaphase spreads of the Atlantic bottlenose dolphin, Tursiops truncatus, to directly compare the evolutionary conservation of chromosomal segments between these two distantly related species. All human chromosomal paints, except the Y probe, hybridized to Tursiops counterparts, and every dolphin chromosome was painted except for the smallest submetacentric pair. In our analysis, 36 segments of conserved synteny common to the human and dolphin genomes were identified. The distribution of conserved chromosomal segments and the specific rearrangement patterns found between the two genomes are presented and discussed.

1.
Bielec PE, Riggs PK, Womack JE, Busbee DL: A high resolution GBG-banded karyotype of the Atlantic bottlenose dolphin, Tursiops truncatus: generation of an ideogram, and NOR localization by fluorescence in situ hybridization. Cytogenet Cell Genet 78:6–11 (1997).
2.
Carvan MJ, Santostefano M, Safe S, Busbee, DL: Characterization of a bottlenose dolphin (Tursiops truncatus) kidney epithelial cell line. J marine Mammal Sci 10:52–69 (1994).
3.
Chowdhary BP, Frönicke L, Gustavsson I, Scherthan H: Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping-based homologies. Mammal Genome 7:297–302 (1996).
4.
Eppig JT, Nadeau JH: Comparative maps: the mammalian jigsaw puzzle. Curr Opin Genet Dev 5:709–716 (1995).
5.
Gallagher DS, Houck ML, Ryan AM, Womack JE, Kumamoto AT: A karyotypic analysis of the lesser Malay chevrotain, Tragulus javanicus (Artiodactyla: Tragulidae). Chrom Res 4:545–551 (1996).
6.
Hameister H, Klett C, Bruch J, Dixkens C, Vogel W, Christenson K: Zoo-FISH analysis: the American mink (Mustela vison) closely resembles the cat karyotype. Chrom Res 5:5–11 (1997).
7.
Hayes H: Chromosome painting with human chromosome-specific DNA libraries reveals the extent and distribution of conserved segments in bovine chromosomes. Cytogenet Cell Genet 71:168–174 (1995).
8.
Lyons L, Raymond M, O’Brien SJ: Comparative genomics: the next generation. Anim Biotechnol 5:103–111 (1994).
9.
Nowak RM: Mammals of the World, 5th Ed, Vol 1, p 1628 (Johns Hopkins University Press, Baltimore 1991).
10.
O’Brien SJ, Cevario SJ, Martenson JS, Thompson MA, Nash WG, Chang E, Graves JAM, Spencer JA, Cho K-W, Tsujimoto H, Lyons LA: Comparative gene mapping in the domestic cat (Felis catus). J Hered 88:408–414 (1997).
11.
O’Brien SJ, Seuanez HN, Womack JE: On the evolution of genome organization in mammals, in MacIntyre RJ (ed): Molecular Evolutionary Genetics, pp 519–589 (Plenum Publishing, London/New York 1985).
12.
Pinkel D, Straume T, Gray JW: Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc natl Acad Sci, USA 83:2934–2938 (1986).
13.
Rappold GA: The pseudoautosomal regions of the human sex chromosomes. Hum Genet 92:315–324 (1993).
14.
Raudsepp T, Frönicke L, Scherthan H, Gustavsson I, Chowdhary BP: Zoo-FISH delineates conserved chromosomal segments in horse and man. Chrom Res 4:218–225 (1996).
15.
Rettenberger G, Bruch J, Fries R, Archibald AL, Hameister H: Assignment of 19 porcine type 1 loci by somatic cell hybrid analysis detects new regions of conserved synteny between human and pig. Mammal Genome 7:275–279 (1996).
16.
Rettenberger G, Klett C, Zechner U, Bruch J, Just W, Vogel W, Hameister H: ZOO-FISH analysis: cat and human karyotypes closely resemble the putative ancestral mammalian karyotype. Chrom Res 3:479–486 (1995a).
17.
Rettenberger G, Klett C, Zechner U, Kunz J, Vogel W, Hameister H: Visualization of the conservation of synteny between humans and pigs by heterologous chromosome painting. Genomics 26:372–378 (1995b).
18.
Richard F, Lombard M, Dutrillaux B: ZOO-FISH suggests a complete homology between human and capuchin monkey (Platyrrhini) euchromatin. Genomics 36:417–423 (1996).
19.
Scherthan H, Cremer T, Árnason Ú, Weir H-U, Lima-de-Faria A, Frönicke L: Comparative chromosome painting discloses homologous segments in distantly related mammals. Nature Genet 6:342–347 (1994).
20.
Sherlock JK, Griffin DK, Delhanty JDA, Parrington JM: Homologies between human and marmoset (Callithrix jacchus) chromosomes revealed by comparative chromosome painting. Genomics 33:214–219 (1996).
21.
Solinas-Toldo S, Lengauer C, Fries R: Comparative genome map of human and cattle. Genomics 27:489–496 (1995).
22.
Stanyon R, Arnold N, Koehler U, Bigoni F, Wienberg J: Chromosomal painting shows that “marked chromosomes” in lesser apes and Old World monkeys are not homologous and evolved by convergence. Cytogenet Cell Genet 68:74–78 (1995).
23.
Trask BJ: Fluorescence in situ hybridization: applications in cytogenetics and gene mapping. Trends Genet 77:149–154 (1991).
24.
Wienberg J, Jauch A, Stanyon R, Cremer T: Molecular cytotaxonomy of primates by chromosomal in situ hybridization. Genomics 8:347–350 (1990).
25.
Wienberg J, Stanyon R: Chromosome painting in mammals as an approach to comparative genomics. Curr Opin Genet Dev 5:792–797 (1995).
26.
Wienberg J, Stanyon R, Jauch A, Cremer T: Homologies in human and Macaca fuscata chromosomes revealed by in situ suppression hybridization with human specific libraries. Chromosoma 101:265–270 (1992).
27.
Wienberg J, Stanyon R, Nash WG, O’Brien PCM, Yang F, O’Brien SJ, Ferguson-Smith MA: Conservation of human vs. feline genome organization revealed by reciprocal chromosome painting. Cytogenet Cell Genet 77:211–217 (1997).
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