Abstract
Introduction: Its wide karyotypic variation characterizes the genus Ctenomys, and in Brazil, the genus is distributed in the country’s southern, Midwest, and northern regions. Recently, populations of Ctenomys have been found in the Midwest and northern Brazil, with two new lineages named C. sp. “xingu” and C. sp. “central.” Methods: This work combines classical cytogenetic and molecular analyses to provide new chromosomal information on the boliviensis group distributed in northern and Midwestern Brazil. This includes the validation of the karyotype of C. bicolor and C. nattereri and the description of the karyotype of C. sp. “xingu” and C. sp. “central.” Results: We found three different karyotypes: 2n = 40 for C. bicolor; 2n = 36 for C. nattereri, and specimens from a locality belonging to C. sp. “central”; 2n = 34 for the lineage C. sp. “xingu” and specimens from a locality belonging to C. sp. “central.” Furthermore, GTG banding revealed homologous chromosomes between species/lineages and allowed the identification of the rearrangements that occurred, which proved the occurrence of fissions. Conclusion: Considering our results on the variation of 2n in the boliviensis group, we found two possibilities: the first, deduced by parsimony, is that 2n = 36 appeared initially, and two fissions produced gave rise to 2n = 40, and an independent fusion gave rise to 2n = 34 from 2n = 36; moreover, the second explanation is that all karyotypes arose independently.
Journal Section:
Original Article
References
1.
D’Elía
G
, Teta
P
, Lessa
EP
. A short overview of the systematics of Ctenomys: species limits and phylogenetic relationships
. In: Freitas
TROD.
, Gonçalves
GL
, Maestri
R
, editors. Tuco-tucos
. Cham
: Springer International Publishing
; 2021
. p. 17
–41
.2.
Patton
JL
, Sherwood
SW
. Chromosome evolution and speciation in rodents
. Annu Rev Ecol Syst
. 1983
;14
(1
):139
–58
. .3.
Reig
OA
. Karyotypic repatterning as a triggering factor in cases of explosive speciation
. Evol Biol Unstable Popul
. 1989
:246
–89
.4.
De Freitas
TRO
. Geographic distribution and conservation of four species of the genus Ctenomys in southern Brazil
. Stud Neotrop Fauna Environ
. 1995
;30
(1
):53
–9
. .5.
Malizia
AI
, Zenuto
RR
, Busch
C
. Demographic and reproductive attributes of dispersers in two populations of the subterranean rodent Ctenomys talarum (tuco-tuco)
. Can J Zool
. 1995
;73
(4
):732
–8
. .6.
Lacey
EA
, Wieczorek
JR
. Ecology of sociality in rodents: a ctenomyid perspective
. J Mammal
. 2003
;84
(4
):1198
–211
. .7.
O’Brien
SL
, Tammone
MN
, Cuello
PA
, Lacey
EA
. Multi-year assessment of variability in spatial and social relationships in a subterranean rodent, the highland tuco-tuco (Ctenomys opimus)
. Behav Ecol Sociobiol
. 2021
;75
(6
):93
–13
. .8.
O’Brien
SL
, Tammone
MN
, Cuello
PA
, Lacey
EA
. Facultative sociality in a subterranean rodent, the highland tuco-tuco (Ctenomys opimus)
. Biol J Linn Soc
. 2020
;129
(4
):918
–30
. .9.
Reig
OA
, Kiblisky
P
. Chromosome multiformity in the genus Ctenomys (rodentia, octodontidae): a progress report
; 1969
. https://doi.org/10.1007/BF00331531.10.
Ortells
M
. Phylogenetic analysis of G-banded karyotypes among the South American subterranean rodents of the genus Ctenomys (Caviomorpha: octodontidae), with special reference to chromosomal evolution and speciation
. Biol J Linn Soc
. 1995
;54
(1
):43
–70
. .11.
de Freitas
TRO
. Cytogenetics status of four Ctenomys species in the south of Brazil
. Genetica
. 2006
;126
(1–2
):227
–35
. .12.
Freitas
TRO
. Tuco-tucos (rodentia, octodontidae) in southern Brazil: Ctenomys lami spec. Nov. Separated from C. minutus nehring 1887
. Stud Neotrop Fauna Environ
. 2001
;36
(1
):1
–8
. .13.
Reig
OA
, Bush
C
, Ortells
MO
, Contreras
JR
. An overview of evolution, systematic, population biology, cytogenetics, molecular biology and speciation in Ctenomys
. In: Evolution of subterranean mammals at the organismal and molecular level
. Nevo
E
, Reig
OA
, Alan
R
. Liss
. In: Nevo
E
, Reig
OA
, editors. Evolution of subterranean mammals at the organismal and molecular level. New York
; 1990
; pp 71
–96
.14.
Cook
JA
, Anderson
S
, Yates
TL
. Bolivian mammals 6. The genus Ctenomys (rodentia, ctenomyidae) in the highlands
. Am Mus Novit
. 1990
;2980
:1
–27
.15.
Reig
OA
, Massarini
AI
, Ortells
MO
, Barros
MA
, Tiranti
S
, Dyzenchauz
F
. New karyotypes and C-banding patterns of the subterranean rodents of the genus Ctenomys (Caviomorpha, Octodontidae) from Argentina
. Mammalia
. 1992
;56
(4
):603
–24
. .16.
Freitas
TRO
. Chromosome polymorphism in Ctenomys minutus (Rodentia-Octodontidae)
. Braz J Genet
. 1997
;20
(1
):1
–7
.17.
Massarini
A
, Mizrahi
D
, Tiranti
S
, Toloza
A
, Luna
F
, Schleich
CE
. Extensive chromosomal variation in Ctenomys talarum talarum from the atlantic coast of Buenos Aires province, Argentina (rodentia: octodontidae)
. Mastozool Neotrop
. 2002
;9
(2
):199
–207
.18.
de Freitas
TRO
. Ctenomys lami: the highest chromosome variability in Ctenomys (Rodentia, Ctenomyidae) due to a centric fusion/fission and pericentric inversion system
. Acta Theriol Warsz
. 2007
;52
(2
):171
–80
. .19.
Anderson
S
, Yates
TL
, Cook
JA
. Notes on Bolivian mammals 4: the genus Ctenomys (rodentia, ctenomyidae) in the eastern lowlands
. Am Mus Novit
. 1987
;2891
(2891
):1
–20
.20.
Parada
A
, D’Elía
G
, Bidau
CJ
, Lessa
EP
. Species groups and the evolutionary diversification of tuco-tucos, genus Ctenomys (Rodentia: ctenomyidae)
. J Mammal
. 2011
;92
(3
):671
–82
. .21.
Roratto
PA
, Fernandes
FA
, de Freitas
TRO
. Phylogeography of the subterranean rodent Ctenomys torquatus: an evaluation of the riverine barrier hypothesis
. J Biogeogr
. 2015
;42
(4
):694
–705
. .22.
Upham
NS
, Patterson
BD
. Evolution of caviomorph rodents: a complete phylogeny and timetree for living genera
. Biol Caviomorph Rodents Divers Evol
. 2015
;1
(October
):63
–120
.23.
Caraballo
DA
, Rossi
MS
. Spatial and temporal divergence of the torquatus species group of the subterranean rodent Ctenomys
. Contrib Zool
. 2018
;87
(1
):11
–24
. .24.
Santi
NAD
, Verzi
DH
, Olivares
AI
, Piñero
P
, Álvarez
A
, Morgan
CC
. A new Pleistocene Ctenomys and divergence dating of the hyperdiverse South American rodent family Ctenomyidae
. J Syst Palaeontol
. 2021
;19
(5
):377
–92
. .25.
Leipnitz
LT
, Fornel
R
, Ribas
LEJ
, Kubiak
BB
, Galiano
D
, de Freitas
TRO
. Lineages of tuco-tucos (ctenomyidae: rodentia) from midwest and northern Brazil: late irradiations of subterranean rodents towards the Amazon forest
. J Mamm Evol
. 2020
;27
(1
):161
–76
. .26.
Londoño-Gaviria
M
, Teta
P
, Ríos
SD
, Patterson
BD
. Redescription and phylogenetic position of Ctenomys dorsalis Thomas 1900, an enigmatic tuco tuco (Rodentia, Ctenomyidae) from the Paraguayan Chaco
. Mammalia
. 2019
;83
(3
):227
–36
. .27.
Bidau
CJ
, de Avila-Pires
FD
. On the type locality of Ctenomys bicolor miranda ribeiro, 1914 (Rodentia: ctenomyidae)
. Mastozool Neotrop
. 2009
;16
(2
):445
–7
.28.
Miranda Ribeiro
A
. História natural: zoologia
. Rio de Janeiro
: Commisão de Linhas Telegráphicas Estratégicas de Matto-Grosso ao Amazonas
; 1914
.29.
Sikes
RS
, Animal Care and Use Committee of the American Society of Mammalogists
. Guidelines of the American Society of Mammalogists for the use of wild mammals in research and education
. J Mammal
. 2016
;97
(3
):663
–88
. .30.
Verma
R
, Babu
A
. Human chromosomes: principles & techniques
. Mol Reprod Dev
. 1996
;43
(1
):134
–.31.
Sakamoto
Y
, Zacaro
AA
. LEVAN, an ImageJ plugin for morphological cytogenetic analysis of mitotic and meiotic chromosomes
. 2009
.32.
Patton
JL
. Chromosome studies of certain pocket mice, genus Perognathus (Rodentia: heteromyidae)
. J Mammal
. 1967
;48
(1
):27
–37
. .33.
Gardner
AL
, Patton
J
. Karytypic variation in oryzomyine rodents (cricetinae) with comments on chromosomal evolution in the neotropical cricetine complex
. opmns
. 1976
;1
(1
). .34.
Seabright
M
. The use of proteolytic enzymes for the mapping of structural rearrangements in the chromosomes of man
. Chromosoma
. 1972
;36
(2
):204
–10
. .35.
Sumner
AT
. A simple technique for demonstrating centromeric heterochromatin
. Exp Cell Res
. 1972
;75
(1
):304
–6
. .36.
Howell
WM
, Black
DA
. ( Alabama 35209, USA), 14 January 1980 Effects of anesthesia, surgical manipulation and dehydration on the nucleic acid and protein content of the pituitary and hypothalamus of the frog ∼ InstitutExperientia36
. Birmingham
: Department of Biology, Samford University
; 1980
; p. 1014
–5
.37.
Maddison
WP
, Maddison
DR
. Mesquite: a modular system for evolutionary analysis
. 2021
; p. 2021
.38.
Cook
JAC
, Salazar-Ravo
J
. Heterochromatin variation among the chromosomally diverse tuco-tucos (rodentia : ctenomyidae) from Bolivia
. In: SÁNCHEZ-CORDERO
V
, MEDELLÍN
RA
, editors. Contribuciones Mastozoológicas en homenaje a Bernardo Villa. UNAM
. México
: Instituto de Biología e Instituto de Ecología
; 2004
; pp 129
–42
.39.
Stolz
JFB
, Gonçalves
GL
, Leipnitz
L
, Freitas
TRO
. DNA-based and geometric morphometric analysis to validate species designation: a case study of the subterranean rodent Ctenomys bicolor
. Genet Mol Res
. 2013
;12
(4
):5023
–37
. .40.
Mascheretti
S
, Mirol
PM
, Giménez
MD
, Bidau
CJ
, Contreras
JR
, Searle
JB
. Phylogenetics of the speciose and chromosomally variable rodent genus Ctenomys (Ctenomyidae, Octodontoidea), based on mitochondrial cytochrome b sequences
. Biol J Linn Soc
. 2000
;70
(2
):361
–76
. .41.
Lizarralde
M
, Deferrari
G
, Álvarez
S
, Escobar
J
. Diferenciación evolutiva en Ctenomys magellanicus: variación morfológica, alozímica y consideraciones biogeográficas de 2 formas cromosómicas
. Interciencia
. 2001
;26
(1
):13
–7
.42.
Gimenez
MD
, Bidau
CJ
, Argüelles
CF
, Contreras
JR
. Chromosomal characterization and relationship between two new species of Ctenomys (Rodentia, Ctenomyidae) from northern Córdoba province, Argentina
. Mamm Biol (früher Z für Säugetierkd
. 1999
;64
:91
–106
.43.
de Freitas
TR
. Geographical variation of heterochromatin in Ctenomys flamarioni (Rodentia-Octodontidae) and its cytogenetic relationships with other species of the genus
. Cytogenet Cell Genet
. 1994
;67
(3
):193
–8
. .44.
Massarini
AI
, Barros
MA
, Ortells
MO
, Reig
OA
. Chromosomal polymorphism and small karyotypic differentiation in a group of Ctenomys species from Central Argentina (Rodentia: octodontidae)
. Genetica
. 1991
;83
(2
):131
–44
. .45.
Freygang
CC
, Marinho
JR
, de Freitas
TRO
. New karyotypes and some considerations about the chromosomal diversification of Ctenomys minutus (rodentia: ctenomyidae) on the coastal plain of the Brazilian state of Rio Grande do Sul
. Genetica
. 2004
;121
(2
):125
–32
. .46.
Tomasco
IH
, Lessa
EP
. Phylogeography of the tuco-tuco Ctenomys pearsoni: mtDNA variation and its implications for chromosomal differentiation
. quintessential Nat Honor life Leg Oliver P Pearson
; 2007
; p. 859
–82
.47.
Fernandes
F
, Fernández-Stolz
G
, Lopes
C
, Freitas
T
. The conservation status of the tuco-tucos, genus Ctenomys (Rodentia: ctenomyidae), in southern Brazil
. Braz J Biol
. 2007
;67
(4 Suppl l
):839
–47
. .48.
Buschiazzo
LM
, Caraballo
DA
, Labaroni
CA
, Teta
P
, Rossi
MS
, Bidau
CJ
, et al. Comprehensive cytogenetic analysis of the most chromosomally variable mammalian genus from South America: Ctenomys (Rodentia: caviomorpha: Ctenomyidae)
. Mamm Biol
. 2022
;102
(5–6
):1963
–79
. .© 2024 S. Karger AG, Basel
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