Karyotypes of 3 male Talpa specimens from northern Spain were analyzed. The mesostyles of upper molars and cytochrome b sequence analysis identified these specimens as belonging to Talpa aquitania, a new Talpa species recently described from northern Spain and southern France. We describe here for the first time the karyotype of Talpa aquitania. Its diploid number is 2n = 34 and NFa = 64, and all chromosomes including the sex chromosomes are biarmed, either metacentric or submetacentric. G-banding demonstrated that the karyotypes of T. aquitania and T. occidentalis (the most closely related species) are almost identical. However, the karyotype of T. aquitania differs from the karyotypes of both T. europaea and T. occidentalis in that it has a medium-sized biarmed Y chromosome rather than a dot-like chromosome and that chromosome 16 is submetacentric in T. aquitania but has a small p-arm in both T. europaea and T. occidentalis. Pericentromeric C-bands were scarce and only clearly visible in a few chromosomal pairs. In addition, C-banding demonstrated that half of the 14p, the 16p, and the Y chromosome are all heterochromatic. rDNA genes were located at the secondary constriction in autosomal pair 3, a common feature in the karyotypes of all Talpa species. Hybridization signals for telomeric repeats were found on the telomeres and the pericentric regions of some chromosomes and co-localized in the secondary constriction of pair 3 with the rDNA genes. In conclusion, the karyotype of T. aquitania from northern Spain is very similar to the karyotype of other species belonging to the genus Talpa.

1.
Arslan A, Zima J: Karyotypes of the mammals of Turkey and neighbouring regions: review. Folia Zool 63:1-62 (2014).
2.
Burgos M, Jiménez R, Díaz de la Guardia R: A rapid, simple and reliable combined method for G-banding mammalian and human chromosomes. Stain Technol 61:257-260 (1986).
3.
Ducroz JF, Volobouev V, Granjon L: An assessment of the systematics of arvicanthine rodents using mitochondrial DNA sequences: evolutionary and biogeographical implications. J Mamm Evol 8:173-206 (2001).
4.
Endow SA: Polytenization of the ribosomal genes on the X and Y chromosomes of Drosophila melanogaster. Genetics 100:375-385 (1982).
5.
Fedyk S, Ivanitskaya EY: Chromosomes of Siberian shrews. Acta Theriol 27:475-492 (1972).
6.
Feuda R, Bannikova AA, Zemlemerova ED, Febbraro M, Loy A, et al: Tracing the evolutionary history of the mole, Talpa europaea, through mitochondrial DNA phylogeography and species distribution modelling. Biol J Linn Soc 114:495-512 (2015).
7.
Gornung E, Volleth M, Capanna E, Castiglia R: Comparative cytogenetics of moles (Eulipotyphla, Talpidae): chromosomal differences in Talpa romana and Talpa europaea. Cytogenet Genome Res 121:249-254 (2008).
8.
Hugot JP, Gu SH, Feliu C, Ventur J, Ribas A, et al: Genetic diversity of Talpa europaea and Nova Hanta Virus (NVAV) in France. Bull Acad Vet Fr 167 (2014).
9.
Hutterer R: Order Soricomorpha, in Wilson DE, Reeder DM (eds): Mammal Species of the World. A Taxonomic and Geographic Reference, ed 3, pp 220-311 (The Johns Hopkins University Press, Baltimore 2005).
10.
Ijdo JW, Wells RA, Baldini A, Reeders ST: Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. Nucleic Acids Res 19:4780 (1991).
11.
Jiménez R, Burgos M, Díaz de la Guardia R: Karyotype and chromosome banding in the mole (Talpa occidentalis) from the south-east of the Iberian Peninsula. Implications on its taxonomic position. Caryologia 37:253-258 (1984).
12.
Kawada S, Harada M, Grafodatsky AS, Oda S: Cytogenetic study of the Siberian mole, Talpa altaica (Insectivora: Talpidae) and karyological relationships within the genus Talpa. Mammalia 66:53-62 (2002).
13.
Kryštufek B, Nedyalkov N, Astrin J, Hutterer R: News from the Balkan refugium: Thrace has an endemic mole species (Mammalia: Talpidae). Bonn Zool Bull 67:41-57 (2018).
14.
Meylan A: Données nouvelles sur les chromosomes des insectivores européens. Rev Suiss Zool 75:691-696 (1966).
15.
Nicolas V, Martínez-Vargas J, Hugot JP: Talpa aquitania nov. sp. (Talpidae, Soricomorpha) a new mole species from southwest France and north Spain. Bull Acad Vet Fr 168:329-334 (2015).
16.
Nicolas V, Martínez-Vargas J, Hugot JP: Molecular data and ecological niche modelling reveal the evolutionary history of the common and Iberian blind moles (Talpidae) in Europe. Zoologica Scripta 46:12-26 (2017a).
17.
Nicolas V, Martínez-Vargas J, Hugot JP: Talpa aquitania sp. nov. (Talpidae, Soricomorpha), a new mole species from SW France and N Spain. Mammalia 81:641-642 (2017b).
18.
Rovatsos MT, Marchal JA, Romero-Fernández I, Fernández FJ, Giagia-Athanosopoulou EB, Sánchez A: Rapid, independent, and extensive amplification of telomeric repeats in pericentromeric regions in karyotypes of arvicoline rodents. Chromosome Res 19:869-882 (2011).
19.
Ruíz-Herrera A, Nergadze SG, Santagostino M, Giulotto E: Telomeric repeats far from the ends: mechanisms of origin and role in evolution. Cytogenet Genome Res 122:219-228 (2008).
20.
Selçuk AY, Kefelioğlu H: Cytogenetic characteristic of the Caucasian pygmy shrew (Sorex volnuchini) and Levant mole (Talpa levantis) (Mammalia: Eulipotyphla) in northern Anatolia, Turkey. Turk J Zool 41:963-969 (2017).
21.
Sözen M, Matur F, Çolak F, Irmak S: Karyological characteristics, morphological peculiarities, and a new distribution locality for Talpa davidiana (Mammalia: Soricomorpha) in Turkey. Turk J Zool 36:806-813 (2012).
22.
Sumner AT: A simple technique for demonstrating centromeric heterochromatin. Expl Cell Res 75:304-306 (1972).
23.
Volleth M, Müller S: Zoo-FISH in the European mole (Talpa europaea) detects all ancestral Boreo-Eutherian human homologous chromosome associations. Cytogenet Genome Res 115:154-157 (2006).
24.
Zhdanova NS, Karamishevam TV, Minina J, Astakhova NM, Lansdorp P, et al: Unusual distribution pattern of telomeric repeats in the shrews Sorex araneus and Sorex granaries. Chromosome Res 13:617-625 (2005).
25.
Zurita F, Sánchez A, Burgos M, Jiménez R, Díaz de la Guardia R: Interchromosomal, intercellular and interindividual variability of NORs studied with silver staining and in situ hybridization. Heredity 77:229-223 (1997).
26.
Zurita F, Jiménez R, Burgos M, Díaz de la Guardia R: Sequential silver staining and in situ hybridization reveal a direct association between rDNA levels and the expression of homologous nucleolar organizing regions: a hypothesis for NOR structure and function. J Cell Sci 111:1433-1439 (1998).
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