Insects of the Cercopidae family are widely distributed and comprise 59 genera and 431 species in the New World. They are xylemophagous, causing losses in agricultural and pasture grasses, and are considered as emerging pests. Chromosomally, these insects have been studied by standard techniques, revealing variable diploid numbers and primarily X0 sex chromosome systems (males). We performed chromosome studies in 6 Mahanarva (Cercopidae) species using standard and differential chromosome staining as well as mapping of repetitive DNAs. Moreover, the relationship between the repetitive DNAs was analyzed at the interspecific level. A diploid chromosome number of 2n = 19,X0 was documented, with chromosomes gradually decreasing in size. Neutral or GC-rich regions were detected which varied depending on the species. Fluorescence in situ hybridization with a (TTAGG)n telomeric motif probe revealed terminal signals, matching those of the Cot DNAs obtained from each species, that were also restricted to the terminal regions of all chromosomes. Dot blot analysis with the Cot fraction from M. quadripunctata showed that at least part of the repetitive genome is shared among the 6 species. Our data highlight the conservation of chromosomal features and organization of repetitive DNAs in the genus Mahanarva, suggesting a low differentiation for chromosomes and repetitive DNAs in most of the 6 species studied.

1.
Anjos A, Ruiz-Ruano FJ, Camacho JPM, Loreto V, Cabrero J, et al: U1 snDNA clusters in grasshoppers: chromosomal dynamics and genomic organization. Heredity 114:207-219 (2015).
2.
Bardella VB, Rosa JA, Vanzela AL: Origin and distribution of AT-rich repetitive DNA families in Triatoma infestans (Heteroptera). Infect Genet Evol 23:106-114 (2014).
3.
Bernardo ERA, Rocha VDF, Puga O: Espécies de cigarrinhas-das-pastagens (Hemiptera: Cercopidae) no meio-norte do Mato Grosso. Cienc Rural 33:369-371 (2003).
4.
Boring AM: The chromosomes of the Cercopidae. Biol Bull 24:133-146 (1913).
5.
Cabral-de-Mello DC, Moura RC, Martins C: Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement. Heredity 104:393-400 (2010).
6.
Cabral-de-Mello DC, de Moura RC, de Souza Melo A, Martins C: Evolutionary dynamics of heterochromatin in the genome of Dichotomius beetles based on chromosomal analysis. Genetica 139:315-325 (2011).
7.
Carvalho GS, Webb MD: Cercopid Spittle Bugs of the New World (Hemiptera, Auchenorrhyncha, Cercopidae). (Pensoft, Sofia 2005).
8.
Castanhole MMU, Pereira LLV, de Souza HV, Valério JR, Barbosa LR, Itoyama MM: Meiotic chromosomes and nucleolar behavior in testicular cells of the grassland spittlebugs Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana (Hemiptera, Auchenorrhyncha). Genet Mol Biol 33:244-252 (2010).
9.
Cavalier-Smith T: Skeletal DNA and the evolution of genome size. Annu Rev Biophys Bioeng 11:273-302 (1982).
10.
Charlesworth B, Sniegowski P, Stephan W: The evolutionary dynamics of repetitive DNA in eukaryotes. Nature 371:215-220 (1994).
11.
Cryan JR, Urban JM: Higher-level phylogeny of the insect order Hemiptera: is Auchenorrhyncha really paraphyletic? Syst Entomol 37:7-21 (2012).
12.
Dey SK: Chromosomes of five species of spittle bugs (Homoptera, Cercopidae). Cytologia 56:523-526 (1991).
13.
Dover GA: Molecular drive in multigene families: how biological novelties arise, spread and are assimilated. Trends Genet 2:159-165 (1986).
14.
Elder JF, Turner BJ: Concerted evolution of repetitive DNA sequences in eukaryotes. Q Rev Biol 70:297-320 (1995).
15.
Feschotte C, Pritham EJ: DNA transposons and the evolution of eukaryotic genomes. Annu Rev Genet 41:331-368 (2007).
16.
Frydrychová R, Grossmann P, Trubac P, Vítková M, Marec F: Phylogenetic distribution of TTAGG telomeric repeats in insects. Genome 47:163-178 (2004).
17.
Gokhman VE, Kuznetsova VG: Comparative insect karyology: current state and applications. Entomol Rev 86:352-368 (2006).
18.
Gokhman VE, Anokhin BA, Kuznetsova VG: Distribution of 18S rDNA sites and absence of the canonical TTAGG insect telomeric repeat in parasitoid Hymenoptera. Genetica 142:317-322 (2014).
19.
Golub NV, Kuznetsova VG, Rakitov RA: First karyotype data on the family Myerslopiidae (Hemiptera, Auchenorrhyncha, Cicadomorpha). Comp Cytogenet 8:293-300 (2014).
20.
Golub NV, Golub VB, Kuznetsova VG: Variability of 18S rDNA loci in four lace bug species (Hemiptera, Tingidae) with the same chromosome number. Comp Cytogenet 9:513-522 (2015).
21.
Grozeva S, Kuznetsova VG, Anokhin BA: Karyotypes, male meiosis and comparative FISH mapping of 18S ribosomal DNA and telomeric (TTAGG)n repeat in eight species of true bugs (Hemiptera, Heteroptera). Comp Cytogenet 5:355-374 (2011).
22.
Halkka O: Recombination in six homopterous families. Evolution 18:81-88 (1964).
23.
Ijdo JW, Wells RA, Baldini A, Reeders ST: Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. Nucleic Acids Res 19:4780 (1991).
24.
Kidwell MG: Transposable elements and the evolution of genome size in eukaryotes. Genetica 115:49-63 (2002).
25.
Kuznetsova V, Aguin-Pombo D: Comparative cytogenetics of Auchenorrhyncha (Hemiptera, Homoptera): a review. ZooKeys 538:63-93 (2015).
26.
Kuznetsova VG, Maryanska-Nadachowska A, Nokkala S: A new approach to the Auchenorrhyncha (Hemiptera, Insecta) cytogenetics: chromosomes of the meadow spittlebug Philaenus spumarius (L.) examined using various chromosome banding techniques. Folia Biol 51:33-40 (2003).
27.
Kuznetsova VG, Maryanska-Nadachowska A, Nokkala S: Karyotype characterization of planthopper species Hysteropterum albaceticum Dlabola, 1983 and Agalmatium bilobum (Fieber, 1877) (Homoptera: Auchenorrhyncha : Issidae) using AgNOR-, C- and DAPI/CMA3-banding techniques. Comp Cytogenet 3:111-123 (2009).
28.
Kuznetsova VG, Grozeva SM, Anokhin BA: The first finding of (TTAGG)n telomeric repeat in chromosomes of true bugs (Heteroptera, Belostomatidae). Comp Cytogenet 6:341-346 (2012).
29.
Kuznetsova V, Golub N, Aguin-Pombo D: Karyotypes, B-chromosomes and meiotic abnormalities in 13 populations of Alebra albostriella and A. wahlbergi (Hemiptera, Auchenorrhyncha, Cicadellidae) from Greece. Comp Cytogenet 7:305-325 (2013).
30.
Kuznetsova VG, Grozeva SM, Hartung V, Anokhin BA: First evidence for (TTAGG)n telomeric sequence and sex chromosome post-reduction in Coleorrhyncha (Insecta, Hemiptera). Comp Cytogenet 9:523-532 (2015a).
31.
Kuznetsova VG, Maryanska-Nadachowska A, Anokhin B, Aguin-Pombo D: Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae). Eur J Entomol 112:207-214 (2015b).
32.
López-Flores I, Garrido-Ramos MA: The repetitive DNA content of eukaryotic genomes. Genome Dyn 7:1-28 (2012).
33.
Mandrioli M, Zanasi F, Manicardi GC: Karyotype rearrangements and telomere analysis in Myzus persicae (Hemiptera, Aphididae) strains collected on Lavandula sp. plants. Comp Cytogenet 8:259-274 (2014).
34.
Marin-Morales MA, Zefa E, Bertagna M, Camargo Mathias MI, Arrigoni E: Chromosome analysis of two species of sugar cane pests of the genus Mahanarva (Homoptera, Cercopidae). Caryologia 55:357-360 (2002).
35.
Maryańska-Nadachowska A, Kuznetsova VG, Lachowska D, Drosopoulos S: Mediterranean species of the spittlebug genus Philaenus: modes of chromosome evolution. J Insect Sci 12:54 (2012).
36.
Mattos VF, Cella DM, Carvalho LS, Candido DM, Schneider MC: High chromosome variability and the presence of multivalent associations in buthid scorpions. Chromosome Res 21:121-136 (2013).
37.
Melters DP, Paliulis LV, Korf IF, Chan SWL: Holocentric chromosomes: convergent evolution, meiotic adaptations, and genomic analysis. Chromosome Res 20:579-593 (2012).
38.
Mohan KN, Rani BS, Kulashrestha PS, Kadandale JS: Characterization of TTAGG telomeric repeats, their interstitial occurrence and constitutively active telomerase in the mealybug Planococcus lilacinus (Homoptera; Coccoidea). Chromosoma 120:165-175 (2011).
39.
Monti V, Giusti M, Bizzaro D, Manicardi GC, Mandrioli M: Presence of a functional (TTAGG)n telomere-telomerase system in aphids. Chromosome Res 19:625-633 (2011).
40.
Nguyen P, Sahara K, Yoshido A, Marec F: Evolutionary dynamics of rDNA clusters on chromosomes of moths and butterflies (Lepidoptera). Genetica 138:343-354 (2010).
41.
Paladini A, Carvalho GS: Descrição de três novas espécies de Mahanarva (Hemiptera, Cercopidae, Ischnorhininae). Iheringia. Série Zool 97:57-66 (2007).
42.
Paladini A, Cryan JR: Nine new species of Neotropical spittlebugs (Hemiptera: Cercopidae: Ischnorhininae). Zootaxa 3519:53-68 (2012).
43.
Palomeque T, Lorite P: Satellite DNA in insects: a review. Heredity 100:564-573 (2008).
44.
Panzera Y, Pita S, Ferreiro MJ, Ferrandis I, Lages C, et al: High dynamics of rDNA cluster location in kissing bug holocentric chromosomes (Triatominae, Heteroptera). Cytogenet Genome Res 138:56-67 (2012).
45.
Peck DC, Thompson V: Spittlebugs (Hemiptera: Cercopoidea), in Capinera JL (ed): Encyclopedia of Entomology, pp 3512-3516 (Springer, Dordrecht 2008).
46.
Pinkel D, Straume T, Gray JW: Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934-2938 (1986).
47.
Pita S, Panzera F, Sánchez A, Panzera Y, Palomeque T, Lorite P: Distribution and evolution of repeated sequences in genomes of Triatominae (Hemiptera-Reduviidae) inferred from genomic in situ hybridization. PLoS One 9:e114298 (2014).
48.
Sahara K, Marec F, Traut W: TTAGG telomeric repeats in chromosomes of some insects and other arthropods. Chromosome Res 7:449-460 (1999).
49.
Sambrook JW, Russell D: Molecular Cloning: A Laboratory Manual, ed 3 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 2001).
50.
Schweizer D, Mendelak M, White MJD, Contreras N: Cytogenetics of the parthenogenetic grasshopper Warramaba virgo and its bisexual relatives. X. Patterns of fluorescent banding. Chromosoma 88:227-236 (1983).
51.
Spence JM, Blackman RL, Testa JM, Ready PD: A 169-base pair tandem repeat DNA marker for subtelomeric heterochromatin and chromosomal rearrangements in aphids of the Myzus persicae group. Chromosome Res 6:167-175 (1998).
52.
Vítková M, Král JJJ, Traut W, Zrzavý J, Marec F: The evolutionary origin of insect telomeric repeats, (TTAGG)n. Chromosome Res 13:145-156 (2005).
53.
Zwick MS, Hanson RE, Islam-Faridi MN, Stelly DM, Wing RA, et al: A rapid procedure for the isolation of C0t-1 DNA from plants. Genome 40:138-142 (1997).
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