B chromosomes are supernumerary dispensable parts of the karyotype which appear in some individuals of some populations in some species. Using advanced sequencing technology, we in silico characterized the high-copy DNA composition of Plantago lagopus with and without B chromosomes. The nuclear genome (2.46 pg/2C) was found to be relatively rich in repetitive sequences, with highly and moderately repeated elements making up 68% of the genome. Besides a centromere-specific marker, we identified a B-specific satellite and a repeat enriched in polymorphic A chromosome segments. The B-specific tandem repeat PLsatB originated from sequence amplification including 5S rDNA fragments.

Keywords:
Supernumerary chromosome, 5S rDNA, Satellite repeat, Polymorphic A chromosome segment
1.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 215:403-410 (1990).
2.
Ananiev EV, Phillips RL, Rines HW: Complex structure of knob DNA on maize chromosome 9. Retrotransposon invasion into heterochromatin. Genetics 149:2025-2037 (1998).
3.
Becker SE, Thomas R, Trifonov VA, Wayne RK, Graphodatsky AS, Breen M: Anchoring the dog to its relatives reveals new evolutionary breakpoints across 11 species of the Canidae and provides new clues for the role of B chromosomes. Chromosome Res 19:685-708 (2011).
4.
Dhar MK, Friebe B, Koul AK, Gill BS: Origin of an apparent B chromosome by mutation, chromosome fragmentation and specific DNA sequence amplification. Chromosoma 111:332-340 (2002).
5.
Dolezel J, Greilhuber J, Suda J: Estimation of nuclear DNA content in plants using flow cytometry. Nat Protoc 2:2233-2244 (2007).
6.
Donald TM, Houben A, Leach CR, Timmis JN: Ribosomal RNA genes specific to the B chromosomes in Brachycome dichromosomatica are not transcribed in leaf tissue. Genome 40:674-681 (1997).
7.
Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E: Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049-1051 (1983).
8.
Green DM: Muller‘s ratchet and the evolution of supernumerary chromosomes. Genome 33:818-824 (1990).
9.
Hewitt GM, John B: Parallel polymorphism for supernumerary segments in Chorthippus parallelus (Zetterstedt). I. British populations. Chromosoma 25:319-342 (1968).
10.
Houben A, Wanner G, Hanson L, Verlin D, Leach CR, Timmis JN: Cloning and characterisation of polymorphic heterochromatic segments of Brachycome dichromosomatica. Chromosoma 109:206-213 (2000).
11.
Houben A, Verlin D, Leach CR, Timmis JN: The genomic complexity of micro B chromosomes of Brachycome dichromosomatica. Chromosoma 110:451-459 (2001).
12.
Houben A, Banaei-Moghaddam AM, Klemme S, Timmis JN: Evolution and biology of supernumerary B chromosomes. Cell Mol Life Sci 71:467-478 (2014).
13.
Jo SH, Koo DH, Kim JF, Hur CG, Lee S, et al: Evolution of ribosomal DNA-derived satellite repeat in tomato genome. BMC Plant Biol 9:42 (2009).
14.
Jones RN, Rees H: B Chromosomes, ed 1 (Academic Press, London 1982).
15.
Leach CR, Houben A, Field B, Pistrick K, Demidov D, Timmis JN: Molecular evidence for transcription of genes on a B chromosome in Crepis capillaris. Genetics 171:269-278 (2005).
16.
Ma L, Vu GTH, Schubert V, Watanabe K, Stein N, et al: Synteny between Brachypodium distachyon and Hordeum vulgare as revealed by FISH. Chromosome Res 18:841-850 (2010).
17.
Macas J, Navrátilová A, Mészáros T: Sequence subfamilies of satellite repeats related to rDNA intergenic spacer are differentially amplified on Vicia sativa chromosomes. Chromosoma 112:152-158 (2003).
18.
Maluszynska J, Schweizer D: Ribosomal RNA genes in B chromosomes of Crepis capillari s detected by non-radioactive in situ hybridization. Heredity 62:59-65 (1989).
19.
Martins C, Ferreira IA, Oliveira C, Foresti F, Galetti PM Jr: A tandemly repetitive centromeric DNA sequence of the fish Hoplias malabaricus (Characiformes: Erythrinidae) is derived from 5S rDNA. Genetica 127:133-141 (2006).
20.
Martis MM, Klemme S, Banaei-Moghaddam AM, Blattner FR, Macas J, et al: Selfish supernumerary chromosome reveals its origin as a mosaic of host genome and organellar sequences. Proc Natl Acad Sci USA 109:13343-13346 (2012).
21.
Novak P, Neumann P, Macas J: Graph-based clustering and characterization of repetitive sequences in next-generation sequencing data. BMC Bioinformatics 11:378 (2010).
22.
Novak P, Neumann P, Pech J, Steinhaisl J, Macas J: RepeatExplorer: a Galaxy-based web server for genome-wide characterization of eukaryotic repetitive elements from next-generation sequence reads. Bioinformatics 29:792-793 (2013).
23.
Peacock WJ, Dennis ES, Rhoades MM, Pryor AJ: Highly repeated DNA sequence limited to knob heterochromatin in maize. Proc Natl Acad Sci USA 78:4490-4494 (1981).
24.
Schmidt-Lebuhn AN, Fuchs J, Hertel D, Hirsch H, Toivonen J, Kessler M: An Andean radiation: polyploidy in the tree genus Polylepis (Rosaceae, Sanguisorbeae). Plant Biol (Stuttg) 12:917-926 (2010).
25.
Sharma PK, Koul AK: Genetic diversity among Plantagos. III. Primary trisomy in Plantago lagopus L. Genetica 64:135-138 (1984).
26.
Stitou S, Diaz de La Guardia R, Jiménez R, Burgos M: Inactive ribosomal cistrons are spread throughout the B chromosomes of Rattus rattus (Rodentia, Muridae). Implications for their origin and evolution. Chromosome Res 8:305-311 (2000).
27.
Valente GT, Conte MA, Fantinatti BE, Cabral-de-Mello DC, Carvalho RF, et al: Origin and evolution of B chromosomes in the cichlid fish Astatotilapia latifasciata based on integrated genomic analyses. Mol Biol Evol 31:2061-2072 (2014).
28.
van Vugt J, de Nooijer S, Stouthamer R, de Jong H: NOR activity and repeat sequences of the paternal sex ratio chromosome of the parasitoid wasp Trichogramma kaykai. Chromosoma 114:410-419 (2005).
29.
Vittorazzi SE, Lourenco LB, Del-Grande ML, Recco-Pimentel SM: Satellite DNA derived from 5S rDNA in Physalaemus cuvieri (Anura, Leiuperidae). Cytogenet Genome Res 134:101-107 (2011).
30.
Wilby AS, Parker JS: The supernumerary segment systems of Rumex acetosa. Heredity 60:109-117 (1988).
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2016
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