Chromosome painting and G-banding analyses were used to delimit homologous chromosomal segments among 4 taxa of the hamsters genus Allocricetulus Argyropulo 1932 (Cricetidae, Murоidea, Rodentia) - A. curtatus (2n = 20), A. eversmanni eversmanni, A. eversmanni pseudocurtatus, and the hybrid A. eversmanni beljaevi × A. eversmanni pseudocurtatus (all 2n = 26). Comparative maps between the 4 karyotypes were established based on chromosome painting of chromosome-specific probes from the Syrian hamster (Mesocricetus auratus, 2n = 44). A putative ancestral karyotype for the genus Allocricetulus (AAK) was proposed and contains 12-13 ancestral autosomal elements. Integrated maps demonstrate extended conservation of syntenies within this rodent genus and show the predominant role of Robertsonian rearrangements in the karyotype evolution of the genus Allocricetulus. At the cytogenetic level, we clearly demonstrate karyological differences between karyotypes of species (A. curtatus vs. A. eversmanni) and subspecies A. e. eversmanni and A. e. beljaevi versus A. e. pseudocurtatus, but the karyotypes of A. e. eversmanni and A. e. beljaevi are identical at this level of resolution.

Keywords:
Allocricetulus, Chromosome painting, Fluorescent in situ hybridization, Rodentia
1.
Argyropulo AI: Die Gattungen und Arten der Hamster (Cricetinae Murray, 1866) der Palaearctic. Zeitschrift für Säugetierkunde 20:129-149 (1933).
2.
Dobigny G, Ducroz JF, Robinson TJ, Volobouev V: Cytogenetics and cladistics. Syst Biol 53:470-484 (2004).
3.
Graphodatsky AS, Yang F, O'Brien PC, Perelman P, Milne BS, et al: Phylogenetic implications of the 38 putative ancestral chromosome segments for four canid species. Cytogenet Cell Genet 92:243-247 (2001).
4.
Kartavtseva IV, Vorontsov NN: Chromosome differences among subspecies of hamster Allocricetulus eversmanni (Rodentia, Cricetidae) and the new taxon of subspecific rank description. CIS 53:8-10 (1992).
5.
Matthey R: Chromosomes, heterochromosomes et cytologie comparee des Cricetinae paleartiques (Rodentia). Caryologia 13:199-223 (1960).
6.
Mitina IP: Geographical variability of the hamster Cricetulus eversmanni Br. (Mammalia, Glires). Russ J Zool 37:1869-1875 (1959).
7.
Musser GG, Carleton MD: Superfamily Muroidea, in Wilson DE, Reeder DM (eds): Mammal Species of the World: A Taxonomic and Geographic Reference. Baltimore, pp 894-1532 (Johns Hopkins University Press, Baltimore 2005).
8.
Neumann K, Michaux J, Lebedev V, Yigit N, Colak E, et al: Molecular phylogeny of the Cricetinae subfamily based on the mitochondrial cytochrome b and 12S rRNA genes and the nuclear vWF gene. Mol Phylogenet Evol 39:135-148 (2006).
9.
Pavlinov IY: Rodents - Order Rodentia. Systematics of recent mammals, pp 67-143 (Moscow University Publisher, Moscow 2003).
10.
Romanenko SA, Perelman PL, Serdukova NA, Trifonov VA, Biltueva LS, et al: Reciprocal chromosome painting between three laboratory rodent species. Mamm Genome 17:1183-1192 (2006).
11.
Romanenko SA, Volobouev VT, Perelman PL, Lebedev VS, Serdukova NA, et al: Karyotype evolution and phylogenetic relationships of hamsters (Cricetidae, Muroidea, Rodentia) inferred from chromosomal painting and banding comparison. Chromosome Res 15:283-297 (2007).
12.
Sablina OV, Radjabli SI, Graphodatsky AS: Selected karyotypes, in O'Brien SJ, Nash WG, Menninger JS (eds): ATLAS of Mammalian Karyotypes, pp 207, 208 (John Wiley, Hoboken 2006).
13.
Seabright M: A rapid banding technique for human chromosomes. Lancet 2:971-972 (1971).
14.
Sitnikova NA, Romanenko SA, O'Brien, Perelman PL, Fu B, et al: Chromosomal evolution of Arvicolinae (Cricetidae, Rodentia). I. The genome homology of tundra vole, field vole, mouse and golden hamster revealed by comparative chromosome painting. Chromosome Res 15:447-456 (2007).
15.
Sumner A: A rapid technique for demonstrating centromeric heterochromatin. Exp Cell Res 75:304-306 (1972).
16.
Vinogradov BS, Argyropulo AI: Fauna of the USSR, mammals. Key to Rodents. Moscow-Leningrad: Nauka (1941).
17.
Vorontsov N, Krjukova E: Chromosomes and taxonomic position of the hamsters (genus Allocricetulus) from Zaisan basin with description of a new nation, in Vorontsov N, (ed): The Mammals (Evolution, Karyology, Taxonomy, Fauna), pp 98-100 (Novosibirsk 1969).
18.
Yang F, O'Brien PC, Milne BS, Graphodatsky AS, Solanky N, et al: A complete comparative chromosome map for the dog, red fox, and human and its integration with canine genetic map. Genomics 62:189-202 (1999).
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2013
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