Abstract
Multiple copies of B chromosomes in maize (Zea mays) can accumulate in the genome using the B chromosome’s accumulation mechanism, specifically nondisjunction at the second pollen mitosis and preferential fertilization of the egg. Using this mechanism, we accumulated 4 different-sized minichromosomes derived from the B chromosome to test the chromosome limits of the cell. The accumulation of normal B chromosomes is associated with multiple phenotypes including white stripes and asymmetric leaf blades, but when minichromosomes are accumulated these symptoms are absent. We also found that multiple B chromosome-derived minichromosomes can coexist with A chromosome-derived minichromosomes. During the years that these experiments were conducted, we found many B chromosome rearrangements and fragments, 2 recoverable A chromosome fragments, and observed a minichromosome breakage-fusion-bridge cycle in roots.
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References
1.
Alfenito MR, Birchler JA: Molecular characterization of a maize B chromosome centric sequence. Genetics 135:589–597 (1993).
[PubMed]
2.
Carlson WR: Factors affecting preferential fertilization in maize. Genetics 62:543–554 (1969).
[PubMed]
3.
Carlson WR: A procedure for localizing genetic factors controlling mitotic nondisjunction in the B chromosome of maize. Chromosoma 42:127–136 (1973).
4.
Carlson WR: The B chromosome of corn. Annu Rev Genet 12:5–23 (1978).
[PubMed]
5.
Carlson WR, Chou TS: B chromosome nondisjunction in corn: control by factors near the centromere. Genetics 97:379–389 (1981).
[PubMed]
6.
Carlson WR, Roseman RR: A new property of the maize B chromosome. Genetics 131:211–223 (1992).
[PubMed]
7.
Danilova T, Birchler J: Integrated cytogenetic map of mitotic metaphase chromosome 9 of maize: resolution, sensitivity, and banding paint development. Chromosoma 117:345–356 (2008).
[PubMed]
8.
Han F, Lamb JC, Birchler JA: High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. Proc Natl Acad Sci USA 103:3238–3243 (2006).
[PubMed]
9.
Han F, Gao Z, Yu W, Birchler JA: Minichromosome analysis of chromosome pairing, disjunction, and sister chromatid cohesion in maize. Plant Cell 19:3853–3863 (2007).
[PubMed]
10.
Han F, Gao Z, Birchler JA: Reactivation of an inactive centromere reveals epigenetic and structural components for centromere specification in maize. Plant Cell 21:1929–1939 (2009).
11.
Hanson GP: B-chromosome stimulated crossing over in maize. Genetics 63:601–609 (1969).
12.
Kato A, Zheng YZ, Auger DL, Phelps-Durr T, Bauer MJ, et al: Minichromosomes derived from the B chromosome of maize. Cytogenet Genome Res 109:156–165 (2005).
[PubMed]
13.
Lamb JC, Kato A, Birchler JA: Sequences associated with A chromosome centromeres are present throughout the maize B chromosome. Chromosoma 113:337–349 (2005).
[PubMed]
14.
Lin BY: Regional control of nondisjunction of the B chromosome in maize. Genetics 90:931–935 (1978).
15.
Lin BY: Two new B-10 translocations involved in the control of nondisjunction of the B chromosome in maize. Genetics 92:931–945 (1979).
16.
Longley AE: Supernumerary chromosomes in Zea mays. J Agric Res 35:769–784 (1927).
17.
Masonbrink RE, Birchler JA: Sporophytic nondisjunction of the maize B chromosome at high copy numbers. J Genet Genomics 37:79–84 (2010).
18.
Nel PM: The modification of crossing over in maize by extraneous chromosomal elements. Theor Appl Genet 43:196–202 (1973).
19.
Randolph LF: Genetic characteristics of the B chromosomes in maize. Genetics 26:608–631 (1941).
[PubMed]
20.
Rhoades MM: Studies of a telocentric chromosome in maize with reference to the stability of its centromere. Genetics 25:483–520 (1940).
[PubMed]
21.
Rhoades MM, Dempsey E: On the mechanism of chromatin loss induced by the B-chromosome of maize. Genetics 71:73–96 (1972).
[PubMed]
22.
Rhoades MM, Dempsey E, Ghidoni A: Chromosome elimination in maize induced by supernumerary B chromosomes. Proc Natl Acad Sci USA 57:1626–1632 (1967).
[PubMed]
23.
Roman H: Directed fertilization in maize. Proc Natl Acad Sci USA 34:36–42 (1948).
[PubMed]
24.
Roman H: Factors affecting mitotic nondisjunction in maize. Rec Genet Soc Am 18:112 (1949).
25.
Staub RW: Leaf striping correlated with the presence of B chromosomes in maize. J Hered 78:71–74 (1987).
26.
Ward EJ: Nondisjunction: localization of the controlling site in the maize B chromosome. Genetics 73:387–391 (1973).
[PubMed]
27.
Yu W, Han F, Gao Z, Vega JM, Birchler JA: Construction and behavior of engineered minichromosomes in maize. Proc Natl Acad Sci USA 104:8924–8929 (2007).
[PubMed]
© 2012 S. Karger AG, Basel
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2012
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