Vespertilionidae is the largest chiropteran family that comprises species of different specialization and wide geographic distribution. Up to now, only a few vespertilionid species have been studied by molecular cytogenetic approaches. Here, we have investigated the karyotypic relationships of 4 Vespertilionidae species from Siberia by G-banding and comparative chromosome painting. Painting probes from Aselliscus stoliczkanus were used to establish interspecific homologous chromosomal segments in Myotis dasycneme (2n = 44), Murina hilgendorfi (2n = 44), Plecotus auritus (2n = 32), and Vespertilio murinus (2n = 38). Robertsonian translocations and a few inversions differentiated the karyotypes of the examined species. Painting of P. auritus karyotype with human probes revealed 3 previously undetected cryptic segments homologous to human chromosomes (Homo sapiens, HSA) 8, 15, and 19, respectively. As a consequence, the existence of 2 HSA 4 + 8 syntenies in the P. auritus karyotype has been proven. In addition, a pericentric inversion or centromere shift was revealed on the smallest metacentric P. auritus chromosome 16/17 using the HSA 16 probe explaining the different G-banding pattern in comparison to the homologous Myotis chromosome 16/17.

Ando K, Tagawa T, Uchida TA: Considerations of karyotypic evolution within Vespertilionidae. Experientia 33:877–879 (1977).
Ao L, Gu X, Feng Q, Wang J, O’Brien PC, et al: Karyotypic relationships of six bat species (Chiroptera, Vespertlionidae) from China revealed by chromosome painting and G-banding comparison. Cytogenet Genome Res 115:145–153 (2006).
Ao L, Mao X, Nie W, Gu X, Feng Q, et al: Karyotypic evolution and phylogenetic relationships in the order Chiroptera as revealed by G-banding comparison and chromosomal painting. Chromosome Res 15:257–267 (2007).
Bickham JW: Chromosomal variation and evolutionary relationships of vespertilionid bats. J Mammal 60:350–363 (1979a).
Bickham JW: Banded karyotypes of 11 species of American bats (genus Myotis). Cytologia 44: 789–797 (1979b).
Capanna E, Civitelli MV: Chromosomal mechanisms in the evolution of chiropteran karyotype. Chromosomal tables of Chiroptera. Caryologia 23:79–111 (1970).
Ferguson-Smith MA: Genetic analysis by chromosome sorting and painting: phylogenetic and diagnostic applications. Eur J Hum Genet 5:253–265 (1997).
Graphodatsky AS, Yang F, Serdukova N, Perelman P, Zhdanova N, Ferguson-Smith MA: Dog chromosome-specific paints reveal evolutionary inter- and intrachromosomal rearrangements in the American mink and human. Cytogenet Cell Genet 90:275–278 (2000).
Gu X, He S, Ao L: Molecular phylogenetics among three families of bats (Chiroptera: Rhinolophidae, Hipposideridae, and Vespertilionidae) based on partial sequences of the mitochondrial 12S and 16S rRNA genes. Zoological Studies 47:368–378 (2008).
Harada M: Karyotypic evolution in the family Vespertilionidae. Mamm Sci 28:69–83 (1988).
Harada M, Ando K, Uchida TA, Takada S: A karyological study on two Japanese species of Murina (Mammalia: Chiroptera). J Mammal Soc Japan 12:15–23 (1987).
Hoofer SR, Van Den Bussche RA: Molecular phylogenetics of the chiropteran family Vespertilionidae. Acta Chiropterologia 5:1–63 (2003).
Mao X, Nie W, Wang J, Su W, Ao L, et al: Karyotype evolution in Rhinolophus bats (Rhinolophidae, Chiroptera) illuminated by cross-species chromosome painting and G-banding comparison. Chromosome Res 15:835–848 (2007).
Mao X, Nie W, Wang J, Su W, Feng Q, et al: Comparative cytogenetics of bats (Chiroptera). The prevalence of Robertsonian translocations limits the power of chromosomal characters in resolving interfamily phylogenetic relationships. Chromosome Res 16:155–170 (2008).
Mao X, Wang J, Su W, Wang Y, Yang F, Nie W: Karyotypic evolution in family Hipposideridae (Chiroptera, Mammalia) revealed by comparative chromosome painting, G- and C-banding. Zoolog Res 31:453–460 (2010).
Miller-Butterworth CM, Murphy WJ, O’Brien SJ, Jacobs DS, Springer MS, Teeling EC: A family matter: conclusive resolution of the taxonomic position of the long-fingered bats, Miniopterus. Mol Biol Evol 24:1553–1561(2007).
Richards LR, Rambau RV, Lamb JM, Taylor PJ, Yang F, et al: Cross-species chromosome painting in bats from Madagascar: the contribution of Myzopodidae to revealing ancestral syntenies in Chiroptera. Chromosome Res 18:635–653 (2010).
Roehrs ZP, Lack JP, Van Den Bussche RA: Tribal phylogenetic relationships within Vespertilioninae (Chiroptera: Vespertilionidae) based on mitochondrial and nuclear sequence data. J Mammal 91:1073–1092 (2010)
Seabright M: A rapid banding technique for human chromosomes. Lancet 2:971–972 (1971).
Stadelmann B, Jacobs D, Schoeman C, Ruedi M: Phylogeny of African Myotis bats (Chiroptera, Vespertilionidae) inferred from cytochrome b sequences. Acta Chiropterologica 6:177–192 (2004).
Simmons NB: Order Chiroptera, in Wilson DE, Reeder DM (eds): Mammal Species of the World. A Taxonomic and Geographic Reference, 3rd ed., pp 312–530 (John Hopkins University Press, Washington, DC 2005).
Volleth M: Chromosomal homologies of the genera Vespertilio, Plecotus and Barbastella (Chiroptera: Vespertilionidae). Genetica 66:231–236 (1985).
Volleth M: Karyotype analysis of Murina suilla and Phoniscus atrox from Malaysia (Chiroptera: Murininae, Kerivoulinae). Lynx (Praha) 37:275–284 (2006).
Volleth M, Heller KG: Phylogenetic relationships of vespertilionid genera (Mammalia: Chiroptera) as revealed by karyological analysis. Z Zool Syst Evol 32:11–34 (1994).
Volleth M, Klett C, Kollak A, Dixkens C, Winter Y, et al: ZOO-FISH analysis in a species of the order Chiroptera: Glossophaga sorcina (Phyllostomidae). Chromosome Res 7:57–64 (1999).
Volleth M, Heller KG, Pfeiffer RA, Hameister H: A comparative ZOO-FISH analysis in bats elucidates the phylogenetic relationships between Megachiroptera and five Microchiropterian families. Chromosome Res 10:477–497 (2002).
Yang F, Graphodatsky AS, O’Brien PC, Colabella A, Solanky N, et al: Reciprocal chromosome painting illuminates the history of genome evolution of the domestic cat, dog and human. Chromosome Res 8:392–404 (2000).
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.