Stripe rust (Puccinia striiformis tritici (Pst)) is one of the most destructive diseases of wheat in the world. Exploiting and utilizing stripe rust resistance genes of wild species has become an essential strategy for resistance breeding. Psathyrostachyshuashanica Keng ex Kuo is a wild species in Triticeae that has been used for wheat improvement because of its high resistance or immunity to stripe rust. In this study, 9 wheat-P. huashanica addition lines were characterized by Giemsa C-banding, genomic in situ hybridization (GISH), and disease resistance evaluation. Giemsa C-banding and GISH demonstrated that lines 163-5, 165-1, 183-5, 240-3, and 240-4 are P. huashanica 3Ns chromosome monosomic addition lines; lines 183-1 and 183-20 are P. huashanica 3Ns chromosome disomic addition lines; line 165-20 is a P. huashanica 3Ns and 4Ns chromosomes double disomic addition line, and line 219-1 is a P. huashanica 1Ns and 3Ns/5A chromosomes double disomic addition-substitution line. All these addition lines with P. huashanica 3Ns chromosome(s) expressed high resistance or immunity to stripe rust. By comparing the series of wheat-P. huashanica chromosome addition lines, we concluded that the P. huashanica 3Ns chromosome carries the gene(s) for resistance or immunity to stripe rust. These addition lines can be used as a donor source of novel stripe rust resistance to wheat breeding programs.

Ananiev EV, Riera Lizarazu O, Rines HW, Phillips RL: Oat-maize chromosome addition lines: a new system for mapping the maize genome. Proc Natl Acad Sci USA 94:3524–3529 (1997).
Baden C: A taxonomic revision of Psathyrostachys (Poaceae). Nord J Bot 11:3–26 (1991).
Bao Y, Li X, Liu S, Cui F, Wang HG: Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium partial amphiploid resistance to powery mildew and stripe rust. Cytogenet Genome Res 126:390–395 (2009).
Cai X, Chen PD, Xu SS, Oliver RE, Chen X: Utilization of alien genes to enhance Fusarium head blight resistance in wheat – a review. Euphytica 142:309–318 (2005).
Cao ZJ, Wang XP, Wang MN, Cao SH, Jing JX, et al: Genetic analysis and molecular markers of a novel stripe rust resistance gene YrHua in wheat originated from Psathyrostachys huashanica Keng. Acta Genet Sin 32:738–742 (2005).
Chang SB, Jong HD: Production of alien chromosome additions and their utility in plant genetics. Cytogenet Genome Res 109:335–343 (2005).
Chen Q, Friebe B, Conner RL, Laroche A, Thomas JB, Gill BS: Molecular cytogenetic characterization of Thinopyrum intermedium – derived wheat germplasm specifying resistance to wheat streak mosaic virus. Theor Appl Genet 96:1–7 (1998).
Chen SY, Zhang AJ, Fu J: The hybridization between Triticum aestivum and Psathyrostachys huashanica. Acta Genet Sin 18:508–512 (1991).
Chen XM: Epidemiology and control of stripe rust [Puccinia striiformis f. sp. Tritici] on wheat. Can J Plant Pathol 27:314–337 (2005).
Cho S, Garvin DF, Muehlbauer GJ: Transcriptome analysis and physical mapping of barley genes in wheat – barley chromosome addition lines. Genetics 172:1277–1285 (2006).
Dosba F, Doussinault G, Rivoal R: Extraction, identification and utilization of the addition lines T. aestivum- Ae.ventricosa. Proc 5th Int Wheat Genet Symp, Indian Society of Genetics and Plant Breeding, pp 332–337 (1978).
Doussinault G, Delibes A, Sanchez-Monge R, Garcia-Olmedo F: Transfer of a dominant gene for resistance to eyespot disease from a wild grass to hexaploid wheat. Nature 303:698–700 (1983).
Doyle JJ, Doyle JL: Isolation of plant DNA from fresh tissue. Focus 12:13–15 (1990).
Fedak G: Molecular aids for integration of alien chromatin through wide crosses. Genome 42:584–591 (1999).
Friebe B, Gill BS: C-band polymorphism and structural rearrangements detected in common wheat (Triticum aestivum). Euphytica 78:1–5 (1994).
Friebe B, Tuleen NA, Badaeva ED, Gill BS: Cytogenetic identification of Triticum peregrinum chromosomes added common wheat. Genome 39:272–276 (1996).
Friebe B, Qi LL, Nasuda S, Zhang P, Tuleen NA, Gill BS: Development of a complete set of Triticum aestivum - Aegilops speltoides chromosome addition lines. Theor Appl Genet 101:51–58 (2000).
Fu J, Zhao JX, Chen SY, Hou WS, Yang QH: Studies on molecular cytogenetics of wheat – Psathyrostachys huashanica germplasm with resistance to wheat take-all fungus. Acta Bot Boreal-Occident Sin 23:1905–1909 (2003).
Gill BS, Friebe B, Endo TR: Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome 34:830–839 (1991).
Han FP, He MY, Hao S, Ma YZ, Xin ZY: Variation of wheatgrass chromosomes in wheat-wheatgrass disomic addition line TA1–14 revealed by fluorescence in situ hybridization. Acta Bot Sin 40:33–36 (1998a).
Han FP, Zhang XQ, Bu XL, He MY, Hao S, et al: Variation of wheatgrass chromosomes in wheat-wheatgrass alien addition line ‘TA1–27’ revealed by fluorescence in situ hybridization (FISH). Sci China Ser C 41:366–371 (1998b).
Hu C, Hole DJ, Albrechtsen RS, Hu CJ: Barley chromosome location and expression of dwarf bunt resistance in wheat addition lines. Plant Dis 80:1273–1276 (1996).
Jauhar PP, Peterson TS, Xu SS: Cytogenetic and molecular characterization of a durum alien disomic addition line with enhanced tolerance to Fusarium head blight. Genome 52:467–483 (2009).
Jiang J, Friebe B, Gill BS: Recent advances in alien gene transfer in wheat. Euphytica 73:199–212 (1994).
Jing JX, Fu J, Yuan HX, Wang MN, Shang HS, Li ZQ: A preliminary study on heredity of the resistance to stripe rust in three wild relatives of wheat. Acta Phytopathol Sin 29:147–150 (1999).
Kang HY, Zang HQ, Fan X, Zhou YH: Morphological and cytogenetic studies on the hybrid between common wheat and Psathyrostachys huashanica Keng ex Kuo. Euphytica 162:441–448 (2008).
Kang HY, Wang Y, Sun GL, Zhang HQ, Fan X, Zhou YH: Production and characterization of an amphiploid between common wheat and Psathyrostachys huashanica Keng ex Kuo. Plant Breed 128:36–40 (2009).
Khan IA: Molecular and agronomic characterization of wheat-Agropyron intermedium recombinant chromosomes. Plant Breed 119:25–29 (2000).
Knott DR, Dv&ocaron;ák J, Nanda JS: The transfer to wheat and homology of an Agropyron elongatum chromosome carrying a resistance gene to stem rust. Can J Genet Cytol 19:75–79 (1977).
Kuo PC: Flora Reipublicae Popularis Sinicae (Science Press, Beijing 1987).
Lammer D, Cai XW, Arterburn M, Chatelain J, Murray T, Jones S: A single chromosome addition from Thinopyrum elongatum confers a polycarpic, perennial habit to annual wheat. J Exp Bot 55:1715–1720 (2004).
Li ZQ, Zeng SM: Rusts in China. (Agriculture Press of China, Beijing 2002).
Lin F, Sun Q, Xu SC, Chen XM, Zhang LJ, et al: Identification of wheat – Thinopyrum intermedium alien disomic addition lines conferring resistance to stripe rust. Can J Plant Sci 89:569–574 (2009).
Lukaszewski AJ, Gustafson JP: Cytogenetics of Triticale, in Janick J (ed): Plant Breeding Reviews Vol. 5, pp 41–93 (AVI Publishing, New York 1987).
Luo PG, Ren ZL, Zhang HQ, Zhang HY: Identification, chromosome location, and diagnostic markers for a new gene (YrCN19) for resistance to wheat stripe rust. Phytopathology 95:1266–1270 (2005).
McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, et al: Catalogue of Gene Symbols for Wheat. Proc 11th Int Wheat Genet Symp, Brisbane, pp 24–29 (2008).
Muehlbauer GJ, Riera Lizarazu O, Kynast RG, Martin D, Phillips RL, Rines HW: A maize chromosome 3 addition line of oat exhibits expression of the maize homeobox gene liguleless-3 and alteration of cell fates. Genome 43:1055–1064 (2000).
Okagaki RJ, Kynast RG, Livingston SM, Russell CD, Rines HW, Phillips RL: Mapping maize sequences to chromosomes using oat-maize chromosome addition materials. Plant Physiol 125:1228–1235 (2001).
Riley R, Chapman V, Johnson G: Introduction of yellow rust resistance of Aegilops comosa into wheat by genetically induced homoeologous recombination. Nature 217:383–384 (1968).
Schneider A, Molnár I, Molnár-Láng M: Utilization of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat. Euphytica 163:1–19 (2008).
Sears ER: The transfer of leaf-rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp Biol 9:1–22 (1956).
Sharma HC: How wide can a wide cross be? Euphytica 82:43–64 (1995).
Sharma HC, Gill BS: New hybrids between Agropyron and wheat. 2. Production, morphology and cytogenetic analysis of F1 hybrids and backcross derivatives. Theor Appl Genet 66:111–121 (1983).
Shepherd KW, Islam AKMR: Fourth compendium of wheat-alien chromosome lines. Proc 7th Int Wheat Genet Symp, Cambridge, pp 1373–1398 (1988).
Sun GL, Yan J, Yang JL: Production and cytogenetic study of intergeneric hybrid between Triticum aestivum and Psathyrostachys species. Acta Genet Sin 19:205–210 (1992).
Uauy C, Brevis JC, Chen XM, Khan I, Jackson L, et al: High-temperature adult- plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1. Theor Appl Genet 112:97–105 (2005).
van Heusden AW, Shigyo M, Tashiro Y, Vrielink-van Ginkel R, Kik C: AFLP linkage group assignment to the chromosomes of Allium cepa L. via monosomic addition lines. Theor Appl Genet 100:480–486 (2000).
Wang HQ, Yang XB, Ma ZH: Long-distance spore transport of wheat rust pathogen from Sichuan, Yunnan, and Guizhou in southwestern China. Plant Dis 94:873–880 (2010).
Wang MN, Shang HS: Evaluation of resistance in Psathyrostachys huashanica to wheat take-all fungus. Acta Univ Agri Boreal-Occident 28:69–71 (2000).
Wang XE, Li WL, Liu DJ: C-banding of two Psathyrostachys species. J Nanjing Agri Univ 21:10–13 (1998).
Zhao JX, Chen XH, Wang XL, Wu J, Fu J, et al: Molecular cytogenetic study on the alien substitution lines of Triticum-Psathyrostachys. Acta Bot Boreal-Occident Sin 24:2277–2281 (2004a).
Zhao JX, Chen XH, Wang XL, Wu J, Fu J, et al: Molecular cytogenetic study on the alien addition lines of Triticum-Psathyrostachys. J Northwest Sci-Tech Univ Agri For (Nat Sci) 32:105–109 (2004b).
Zhao JX, Ji WQ, Wu J, Chen XH, Cheng XN, et al: Development and identification of a wheat-Psathyrostachys huashanica addition line carrying HMW-GS, LMW-GS and gliadin genes. Genet Resour Crop Evol 57:387–394 (2009).
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.