We report here on the physical mapping of the H1 histone genes (hisDNA) and the 5S ribosomal DNA (rDNA) in 3 Neotropical fish species of the genus Astyanax(A. altiparanae, A. bockmanni and A. fasciatus) and the comparative analysis of the chromosomes bearing these genes. Nucleotide analyses by sequencing of both genes were also performed. The distribution of the H1 histone genes was more conserved than that of the rRNA genes, since these were always located in the pericentromeric regions of 2 chromosome pairs. 5S rDNA was found on one of the pairs that presented an H1 histone cluster; this seems to be a conserved chromosomal feature of the genus Astyanax. In addition, individuals of A. bockmanni and A. fasciatus showed clusters of 5S rDNA on 1 pair of acrocentric chromosomes, not found in A. altiparanae. The results obtained by chromosome mapping as well as by sequencing of both genes showed that A.bockmanni is more closely related to A. fasciatus than to A. altiparanae. The results allow the characterization of cytogenetic markers for improved elucidation of the processes involved in karyotype differentiation of fish genomes.

Almeida-Toledo LF, Ozouf-Costaz C, Foresti F, Bonillo C, Porto-Foresti F, Daniel-Silva MFZ: Conservation of the 5S-bearing chromosome pair and co-localization with major rDNA clusters in five species of Astyanax (Pisces, Characidae). Cytogenet Genome Res 97:229–233 (2002).
Andrews MT, Vaughn JC, Perry BA, Bagshaw JC: Interspersion of histone and 5S RNA genes in Artemia. Gene 51:61–67 (1987).
Artoni RF, Shibatta OA, Gross MC, Schneider CH, Almeida MC, et al: Astyanax aff. fasciatus Cuvier, 1819 (Teleostei; Characidae): evidences of a species complex in the upper rio Tibagi basin (Paraná, Brazil). Neotrop Ichthyol 4:197–202 (2006).
Barzotti R, Pelliccia F, Bucciarelli E, Rocchi A: Organization, nucleotide sequence, and chromosomal mapping of a tandemly repeated unit containing the four core histone genes and a 5S rRNA gene in an isopod crustacean species. Genome 43:341–345 (2000).
Buckup PA, Menezes NA, Ghazzi MS: Catálogo das espécies de peixes de água doce do Brasil. Museu Nacional, Universidade Federal do Rio de Janeiro, Rio de Janeiro (2007).
Cabrero J, López-León MD, Teruel M, Camacho JPM: Chromosome mapping of H3 and H4 histone gene clusters in 35 species of acridid grasshoppers. Chromosome Res 17:397–404 (2009).
Camacho JPM: B chromosomes, in Gregory TR (ed): The Evolution of the Genome (Elsevier, San Diego 2005).
Casciotta JR, Almirón AE, Azpelicueta MM: Astyanax pampa (Characiformes, Characidae), a new species from the southernmost boundary of the Brazilian subregion, Argentina. Revue Suisse Zoologie 112:401–408 (2005).
Childs G, Maxson R, Cohn RH, Kedes L: Orphons: dispersed genetic elements derived from tandem repetitive genes of eukaryotes. Cell 23:651–663 (1981).
Ferro DAM, Neo DM, Moreira-Filho O, Bertollo LAC: Nucleolar organizing regions, 18S and 5S rDNA in Astyanax scabripinnis (Pisces, Characidae): populations distribution and functional diversity. Genetica 110:55–62 (2000).
Foresti F, Oliveira C, Almeida-Toledo LF: A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine. Experientia 49:810–813 (1993).
Garutti V, Britski HA: Descrição de espécie nova de Astyanax (Teleostei, Characidae) da bacia do alto rio Paraná e considerações sobre as demais espécies do gênero na bacia. Comun Mus Ciênc Tecnol 13:65–88 (2000).
Graphodatsky AS, Kukekova AV, Yudkin DV, Trifonov VA, Vorobieva NV, et al: The proto-oncogene C-KIT maps to canid B-chromosomes. Chromosome Res 13:113–122 (2005).
Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Series 41:95–98 (1999).
Hashimoto DT, Porto-Foresti F: Chromosome polymorphism of heterochromatin and nucleolar regions in two populations of the fish Astyanax bockmanni (Teleostei: Characiformes). Neotrop Ichthyol, in press.
Hashimoto DT, Gonçalves VR, Bortolozzi J, Foresti F, Porto-Foresti F: First report of a B chromosome in a natural population of Astyanax altiparanae (Characiformes, Characidae). Genet Mol Biol 31(suppl):275–278 (2008).
Kavalco KF, Pazza R, Almeida-Toledo LF: Astyanaxbockmanni Vari and Castro, 2007: an ambiguous karyotype in the Astyanax genus. Genetica 136:135–139 (2009).
Langeani F, Castro RMC, Oyakawa OT, Shibatta OA, Pavanelli CS, Casatti L: Diversidade da ictiofauna do Alto Rio Paraná: composição atual e perspectivas futuras.Biota Neotrop 7:181–197 (2007).
Levan A, Fredga K, Sandberg AA: Nomenclature for centromeric position on chromosomes. Hereditas 52:201–220 (1964).
Lima FCT, Malabarba LR, Buckup PA, Silva JFP, Vari RP, et al: Genera incertae sedis in Characidae, in Reis RE, Kullander SO, Ferraris CJ (eds): Check List of the Freshwater Fishes of South and Central America, pp 106–169 (Edipucrs, Porto Alegre 2003).
Lucchini S, Nardi I, Barsacchi G, Batistoni R, Andronico F: Molecular cytogenetics of the ribosomal (18S + 28S and 5S) DNA loci in primitive and advanced urodele amphibians. Genome 36:762–773 (1993).
Mäkinem A, Zijlstra C, De Haan NA, Mellink CHM, Bosma AA: Localization of 18S plus 28S and 5S ribosomal RNA genes in the dog by fluorescence in situhybridization. Cytogenet Cell Genet 78:231–235 (1997).
Martins C, Wasko AP: Organization and evolution of 5S ribosomal DNA in the fish genome. Chapter X, in Williams CR (ed): Focus on Genome Research (Nova Science Publishers, New York 2004).
Mestriner CA, Galetti PM Jr, Valentini SR, Ruiz IRG, Abel LDS, et al: Structural and functional evidence that a B chromosome in the characid fish Astyanax scabripinnis is an isochromosome. Heredity 85:1–9 (2000).
Moreira-Filho O, Bertollo LAC: Astyanax scabripinnis (Pisces, Characidae): a species complex. Brazil J Genet 14:331–357 (1991).
Moreira-Filho O, Galetti PM Jr, Bertollo LAC: B chromosomes in the fish Astyanax scabripinnis (Characidae, Tetragonopterinae): an overview in natural populations. Cytogenet Genome Res 106:230–234 (2004).
Oliveira C, Almeida-Toledo LF, Foresti F, Britski HA, Toledo-Filho SA: Chromosome formulae of Neotropical freshwater fishes. Rev Brasil Genet 11:577–624 (1988).
Oliveira C, Foresti F, Hilsdorf AWS: Genetics of Neotropical fish: from chromosomes to populations. Fish Physiol Biochemist 35:81–100 (2009).
Page LM, Burr BM: A Field Guide to Freshwater Fishes. North America North of Mexico (Houghton Mifflin Co., Boston 1991).
Pazza R, Kavalco KF, Bertollo LAC: Chromosome polymorphism in Astyanax fasciatus (Teleostei, Characidae). 1. Karyotype analysis, Ag-NORs and mapping of the 18S and 5S ribosomal genes in sympatric karyotypes and their possible hybrid forms. Cytogenet Genome Res 112:313–319 (2006).
Pendás AM, Morán P, García-Vázquez E: Organization and chromosomal location of the major histone cluster in brown trout, Atlantic salmon and rainbow trout. Chromosoma 103:147–152 (1994a).
Pendás AM, Moran P, Freije JP, Garcia-Vazquez E: Chromosomal mapping and nucleotide sequence of two tandem repeats of Atlantic salmon 5S rDNA. Cytogenet Cell Genet 67:31–36 (1994b).
Reis RE, Kullander SO, Ferraris CJ Jr (eds): Check List of the Freshwater Fishes of South and Central America (Edipucrs, Porto Alegre 2003).
Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425 (1987).
Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599 (2007).
Teruel M, Cabrero J, Perfectti F, Camacho JPM: B chromosome ancestry revealed by histone genes in the migratory locust. Chromosoma 119:217–225 (2010).
Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 (1994).
Vari RP, Castro RMC: New species of Astyanax (Ostariophysi: Characiformes: Characidae) from the Upper Rio Paraná System, Brazil. Copeia 1:150–162 (2007).
Vicari MR, Artoni RF, Moreira-Filho O, Bertollo LAC: Colocalization of repetitive DNAs and silencing of major rRNA genes. A case report of the fish Astyanax janeiroensis. Cytogenet Genome Res 122:67–72 (2008a).
Vicari MR, Noleto RB, Artoni RF, Moreira-Filho O, Bertollo LAC: Comparative cytogenetics among species of the Astyanax scabripinnis complex. Evolutionary and biogeographical inferences. Genet Mol Biol 31:173–179 (2008b).
Vicente VE, Moreira-Filho O, Camacho JPM: Sex-ratio distortion associated with the presence of a B chromosome in Astyanax scabripinnis (Teleostei, Characidae). Cytogenet Cell Genet 74:70–75 (1996).
Yang F, O’Brien PC, Milne BS, Graphodatsky AS, Solanky N, et al: A complete comparative chromosome map for the dog, red fox, and human and its integration with canine genetic maps. Genomics 62:189–202 (1999).
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