Meiosis-specific protein SYCP3 is a major structural component of synaptonemal complex (SC) lateral elements. SYCP3 is rather well conserved in vertebrates. However, some differences in SYCP3 expression have been shown among mammals. To clarifiy these differences, we have investigated SYCP3 expression with the aid of bioinformatic, biochemical and cell biological methods. Here we show that, in contrast to other vertebrates, rat and mouse SYCP3 exist in 2 isoforms. The short isoform is conserved among vertebrates. However, the longer isoform, which represents an N-terminal extension of the shorter one, most likely appeared about 15 million years ago in a common ancestor of rat and mouse and after separation from the hamster branch.

Keywords:
Isoforms, Mammals, Meiosis, SYCP3, Synaptonemal complex
1.
Baier A, Alsheimer M, Volff JN, Benavente R: Synaptonemal complex protein SYCP3 of the rat: evolutionarily conserved domains and the assembly of higher order structures. Sex Dev 1:161–168 (2007a).
2.
Baier A, Alsheimer M, Benavente R: Synaptonemal complex protein SYCP3: conserved polymerization properties among vertebrates. Biochim Biophys Acta 1774:595–602 (2007b).
3.
Bolcun-Filas E, Costa Y, Speed R, Taggart M, Benavente R, et al: SYCP2 is required for synaptonemal complex assembly, DSB repair and homologous recombination. J Cell Biol 176:741–747 (2007).
4.
Bolcun-Filas E, Hall E, Speed R, Taggart M, Grey C, et al: Mutation of the mouse Syce1 gene disrupts synapsis and suggests a link between synaptonemal complex structural components and DNA repair. PLoS Genet 5:e1000393 (2009).
5.
Botelho RJ, DiNicolo L, Tsao N, Karaiskakis A, Tarsounas M, et al: The genomic structure of SYCP3, a meiosis-specific gene encoding a protein of the chromosome core. Biochim Biophys Acta 1518:294–299 (2001).
6.
Costa Y, Cooke HJ: Dissecting the mammalian synaptonemal complex using targeted mutations. Chromosome Res 15:579–589 (2007).
7.
Costa Y, Speed R, Öllinger R, Alsheimer M, Semple CA, et al: Two novel proteins recruited by synaptonemal complex protein 1 (SYCP1) are at the centre of meiosis. J Cell Sci 118:2755–2762 (2005).
8.
Cox J, Mann M: MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat Biotechnol 26:1367–1372 (2008).
9.
Cox J, Matic I, Hilger M, Nagaraj N, Selbach M, et al: A practical guide to the MaxQuant computational platform for SILAC-based quantitative proteomics. Nat Protoc 4:698–705 (2009).
10.
de Vries FA, de Boer E, van den Bosch M, Baarends WM, Ooms M, et al: Mouse Sycp1 functions in synaptonemal complex assembly, meiotic recombination, and XY body formation. Genes Dev 19:1376–1389 (2005).
11.
Dobson MJ, Pearlman RE, Karaiskakis A, Spyropoulos B, Moens PB: Synaptonemal complex proteins: occurrence, epitope mapping and chromosome disjunction. J Cell Sci 107:2749–2760 (1994).
12.
Hamer G, Gell K, Kouznetsova A, Novak I, Benavente R, Höög C: Characterization of a novel meiosis-specific protein within the central element of the synaptonemal complex. J Cell Sci 119:4025–4032 (2006).
13.
Hamer G, Wang H, Bolcun-Filas E, Cooke HJ, Benavente R, Höög C: Progression of meiotic recombination requires structural maturation of the central element of the synaptonemal complex. J Cell Sci 121:2445–2451 (2008).
14.
Heyting C, Moens PB, van Raamsdonk W, Dietrich AJJ, Vink ACG, Redeker EJW: Identification of two major components of the lateral elements of synaptonemal complexes of the rat. Eur J Cell Biol 43:148–154 (1987).
15.
Ishihama Y, Rappsilber J, Andersen JS, Mann M: Microcolumns with self-assembled particle frits for proteomics. J Chromatogr A 979:233–239 (2002).
16.
Iwai T, Yoshii A, Yokota T, Sakai C, Hori H, et al: Structural components of the synaptonemal complex, SYCP1 and SYCP3, in the medaka fish Oryzias latipes. Exp Cell Res 312:2528–2537 (2006).
17.
Kozak M: The scanning model for translation: an update. J Cell Biol 108:229–241 (1989).
18.
Lammers JH, Offenberg HH, van Aalderen M, Vink AC, Dietrich AJ, Heyting C: The gene encoding a major component of the lateral elements of synaptonemal complexes of the rat is related to X-linked lymphocyte-regulated genes. Mol Cell Biol 14:1137–1146 (1994).
19.
Lammers JH, van Aalderen M, Peters AH, van Pelt AA, Gaemers IC, et al: A change in the phosphorylation pattern of the 30000–33000 Mr synaptonemal complex proteins of the rat between early and mid-pachytene. Chromosoma 104:154–163 (1995).
20.
Liebe B, Alsheimer M, Höög C, Benavente R, Scherthan H: Telomere attachment, meiotic chromosome condensation, pairing and bouquet stage duration are modified in spermatocytes lacking axial elements. Mol Biol Cell 15:827–837 (2004).
21.
Meuwissen RL, Offenberg HH, Dietrich AJ, Riesewijk A, van Iersel M, et al: A coiled-coil related protein specific for synapsed regions of meiotic prophase chromosomes. EMBO J 11:5091–5100 (1992).
22.
Miyamoto T, Hasuike MP, Yogev L, Maduro MR, Ishikawa M, et al: Azoospermia in patients heterozygous for a mutation in SYCP3. Lancet 362:1714–1719 (2003).
23.
Offenberg HH, Schalk JA, Meuwissen RL, van Aalderen M, Kester HA, et al: SCP2: a major protein component of the axial elements of synaptonemal complexes of the rat. Nucleic Acids Res 26:2572–2579 (1998).
24.
Öllinger R, Alsheimer M, Benavente R: Mammalian protein SCP1 forms synaptonemal complex-like structures in the absence of meiotic chromosomes. Mol Biol Cell 16:212–217 (2005).
25.
Olsen JV, de Godoy LM, Li G, Macek B, Mortensen P, et al: Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap. Mol Cell Proteomics 4:2010–2021 (2005).
26.
Page SL, Hawley RS: The genetics and molecular biology of the synaptonemal complex. Annu Rev Cell Dev Biol 20:525–558 (2004).
27.
Perkins DN, Pappin DJ, Creasy DM, Cottrell JS: Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20:3551–3567 (1999).
28.
Rappsilber J, Ishihama Y, Mann M: Stop and go extraction tips for matrix-assisted laser desorption/ionization, nanoelectrospray, and LC/MS sample pretreatment in proteomics. Anal Chem 75:663–670 (2003).
29.
Rappsilber J, Mann M, Ishihama Y: Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Nat Protoc 2:1896–1906 (2007).
30.
Schalk JA, Dietrich AJ, Vink AC, Offenberg HH, van Aalderen M, Heyting C: Localization of SCP2 and SCP3 protein molecules within synaptonemal complexes of the rat. Chromosoma 107:540–548 (1998).
31.
Winkel K, Alsheimer M, Öllinger R, Benavente R: Protein SYCP2 provides a link between transverse filaments and lateral elements of mammalian synaptonemal complexes. Chromosoma 118:259–267 (2009).
32.
Yang F, De La Fuente R, Leu NA, Baumann C, McLaughlin KJ, Wong PJ: Mouse SYCP2 is required for synaptonemal complex assembly and chromosomal synapsis during male meiosis. J Cell Biol 173:497–507 (2006).
33.
Yuan L, Pelttari J, Brundell E, Björkroth B, Zhao J, et al: The synaptonemal complex protein SCP3 can form multistranded, cross-striated fibers in vivo. J Cell Biol 142:331–339 (1998).
34.
Yuan L, Liu JG, Zhao J, Brundell E, Daneholt B, Höög C: The murine SCP3 gene is required for synaptonemal complex assembly, chromosome synapsis, and male fertility. Mol Cell 5:73–83 (2000).
35.
Zheng YH, Rengaraj D, Choi JW, Park KJ, Lee SI, Han JY: Expression pattern of meiosis associated SYCP family members during germ line development in chickens. Reproduction 138:483–492 (2009).
36.
Zickler D, Kleckner N: Meiotic chromosomes: integrating structure and function. Annu Rev Genet 33:603–754 (1999).
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2010
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