We characterized the DTF2 satellite DNA family of the clam Donaxtrunculus and compared its chromosomal localization with cytogenetic data revealed by fluorochrome banding, C-banding, and 28S rDNA FISH. In contrast to the other satellites detected previously in this species, DTF2 is an abundant (2%) GC-rich satellite that exhibits CpG methylation. Sequence characteristics of DTF2 indicate that its evolution is not affected by constraints that might indicate some functional interactions. Fluorescence in situ hybridization revealed subtelomeric location of this satellite on a subset of 14 out of 19 D. trunculus chromosome pairs. The chromomycin A3 (CMA) staining of GC-rich regions on D. trunculus chromosomes revealed a complex banding pattern that overlaps completely with C-bands. In total, only three bands show subtelomeric location, while 13 bands are located interstitially, one of them being coincident with the 28S rDNA hybridization signal. No bands, either CMA positive (GC-rich) or DAPI positive (AT-rich) were detected at centromeric chromosomal positions. Only two of the CMA-positive bands co-localize with the DTF2 satellite, showing a) the presence of small islands of GC-rich repetitive sequences that remained undetected by CMA/C-banding and b) the abundance of DTF2-divergent GC-rich sequences at interstitial chromosomal locations.

Biscotti MA, Canapa A, Olmo E, Barucca M, Teo CH, et al: Repetitive DNA, molecular cytogenetics and genome organization in the King scallop (Pecten maximus). Gene 406:91–98 (2007).
Boron A, Nicki PW, Skuza L, Zielinski R: Cytogenetic characterization of the zebra mussel Dreissena polymorpha (Pallas) from Miedwie lake, Poland. Folia Biologica (Krakow) 52:33–38 (2004).
Cafasso D, Cozzolino S, De Luca P, Chinali G: An unusual satellite DNA from Zamia paucijuga (Cycadales) characterized by two different organisations of the repetitive unit in the plant genome. Gene 311:71–79 (2003).
Canapa A, Barucca M, Cerioni PN, Olmo E: A satellite containing CENP-B box-like motifs is present in the Antarctic scallop Adamussium colbecki. Gene 247:175–180 (2000).
Charlesworth B, Sniegowski PE, Stephan W: The evolutionary dynamics of repetitive DNA in eukaryotes. Nature 37:215–220 (1994).
Cornet M, Soulard C: Chromosome number and karyotype of Donax trunculus L. (Mollusca, Bivalvia, Tellinacea). Genetica 82:93–97 (1990).
Dover GA: Molecular drive in multigene families: how biological novelties arise, spread and are assimilated. Trends Genet 2:159–165 (1986).
Dover GA: Molecular drive. Trends Genet 18:587–589 (2002).
Durfy SJ, Willard HF: Patterns of intra- and interarray sequence variation in alpha-satellite from the human X chromosome: evidence for short-range homogenization of tandemly repeated DNA sequences. Genomics 5:810–821 (1989).
Gaffney PM, Pierce JC, Mackinley AG, Titchen DA, Glenn WK: Pearl, a novel family of putative transposable elements in bivalve mollusks. J Mol Evol 56:308–316 (2003).
Grewal SI, Elgin SC: Transcription and RNA interference in the formation of heterochromatin. Nature 447:399–406 (2007).
Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98 (1999).
Henikoff S, Ahmad K, Malik HS: The centromere paradox: stable inheritance with rapidly evolving DNA. Science 293:1098–1102 (2001).
Hinegardner R: Cellular DNA content of the Mollusca. Comp Biochem Physiol 47A:447–460 (1974).
Huang X, Hu J, Hu X, Zhang C, Zhang L, et al: Cytogenetic characterization of the bay scallop, Argopecten irradians irradians, by multiple staining techniques and fluorescence in situ hybridization. Genes Genet Syst 82:257–263 (2007).
Hurtado NS, Pasantes JJ: Surface spreading of synaptonemal complexes in the clam Dosinia exoleta (Mollusca, Bivalvia). Chromosome Res 13:575–580 (2005).
Insua A, Lopez-Piñon MJ, Mendez J: Characterization of Aequipecten opecularis (Bivalvia, Pectinidae) chromosomes by different staining techniques and fluorescent in situ hybridization. Genes Genet Syst 73:193–200 (1998).
Kourtidis A, Drosopoulou E, Pantzartzi CN, Chintiroglou CC, Scouras ZG: Three new satellite sequences and a mobile element found inside HSP70 introns of the Mediterranean mussel (Mytilus galloprovincialis). Genome 49:1451–1458 (2006).
Kuznetsova IS, Podgornaya O, Ferguson-Smith MA: High-resolution organization of mouse centromeric and pericentromeric DNA. Cytogenet Genome Res 112:248–255 (2006).
López-Flores I, de la Herrán R, Garrido-Ramos MA, Boudry P, Ruiz-Rejón C, Ruiz-Rejón M: The molecular phylogeny of oysters based on a satellite DNA related to transposons. Gene 339:181–188 (2004).
Martínez A, Mariñas L, González-Tizón A, Méndez J: Cytogenetic characterization of Donax trunculus (Bivalvia: Donacidae) by means of karyotyping, fluorochrome banding and fluorescent in situ hybridization. J Moll Stud 68:393–396 (2002).
Martínez-Expósito MJ, Méndez J, Pasantes JJ: Analysis of NORs and NOR-associated heterochromatin in the mussel Mytilus galloprovincialis Lmk. Chromosome Res 5:268–273 (1997).
Martínez-Lage A, González-Tizón A, Méndez J: Characterization of different chromatin types in Mytilus galloprovincialis L. after C-banding, fluorochrome and restriction endonuclease treatments. Heredity 72:242–249 (1994).
Martínez-Lage A, González-Tizón A, Méndez J: Chromosomal markers in three species of the genus Mytilus (Mollusca: Bivalvia). Heredity 74:369–375 (1995).
Martínez-Lage A, Rodríguez F, González-Tizón A, Prats E, Cornudella L, Méndez J: Comparative analysis of different satellite DNAs in four Mytilus species. Genome 45:922–929 (2002).
Martínez-Lage A, Rodríguez-Fariña F, González-Tizón A, Méndez J: Origin and evolution of Mytilus mussel satellite DNAs. Genome 48:247–256 (2005).
Meneveri R, Agresti A, Rocchi M, Marozzi A, Ginelli E: Analysis of GC-rich repetitive nucleotide sequences in great apes. J Mol Evol 40:405–412 (1995).
Meštrović N, Castagnone-Sereno P, Plohl M: Interplay of selective pressure and stochastic events directs evolution of the MEL172 satellite DNA library in root-knot nematodes. Mol Biol Evol 23:2316–2325 (2006).
Odierna G, Aprea G, Barucca M, Canapa A, Capriglione T, Olmo E: Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution in pectinids. Genetica 127:341–349 (2006).
Passamonti M, Mantovani B, Scali V: Characterization of a highly repeated DNA family in Tapetinae species (Mollusca, Bivalvia: Veneridae). Zoological Sci 15:599–605 (1998).
Pauls E, Affonso PR: The karyotype of Nodipecten nodosus (Bivalvia: Pectinidae). Hydrobiologia 420:99–102 (2000).
Petrović V, Plohl M: Sequence divergence and conservation in organizationally distinct subfamilies of Donax trunculus satellite DNA. Gene 362:37–43 (2005).
Plohl M, Cornudella L: Characterization of a complex satellite DNA in the mollusk Donax trunculus: analysis of sequence variation and divergence. Gene 169:157–164 (1996).
Plohl M, Cornudella L: Characterization of interrelated sequence motifs in four satellite DNAs and their distribution in the genome of the mollusk Donax trunculus. J Mol Evol 44:189–198 (1997).
Plohl M, Prats E, Martínez-Lage A, González-Tizón A, Méndez J, Cornudella L: Telomeric localization of the vertebrate-type hexamer repeat (TTAGGG)n in the wedgeshell clam Donax trunculus and other marine invertebrate genomes. J Biol Chem 277:19839–19846 (2002).
Plohl M, Luchetti A, Meštrović N, Mantovani B: Satellite DNAs between selfishness and functionality: structure, genomics and evolution of tandem repeats in centromeric (hetero)chromatin. Gene 409:72–82 (2008).
Pons J, Juan C, Petitpierre E: Higher-order organization and compartmentalization of satellite DNA PIM357 in species of the coleopteran genus Pimelia. Chromosome Res 10:597–606 (2002).
Rice P, Longden I, Bleasby A: EMBOSS: The European Molecular Biology Open Software Suite. Trends Genet 16:276–277 (2000).
Rozas J, Rozas R: DnaSP version 3: an integrated program for molecular population genetics and molecular evolution analysis. Bioinformatics 15:174–175 (1999).
Saavedra C, Bachère E: Bivalve genomics. Aquaculture 256:1–14 (2006).
Schmidt T, Heslop-Harrison JS: Genomes, genes and junk: the large-scale organization of plant chromosomes. Trends Plant Sci 3:195–199 (1998).
Sola L, Gornung E: Classical and molecular cytogenetics of the zebrafish, Danio rerio (Cyprinidae: Cypriniformes): an overview. Genetica 111:397–412 (2001).
Stephan W: Tandem-repetitive noncoding DNA: forms and forces. Mol Biol Evol 6:198–212 (1989).
Stephan W, Cho S: Possible role of natural selection in the formation of tandem-repetitive noncoding DNA. Genetics 136:333–341 (1994).
Thiriot-Quiévreux C: Review of the literature on bivalve cytogenetics in the last ten years. Cah Biol Mar 43:17–26 (2002).
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882 (1997).
Torreiro A, Martínez-Expósito MJ, Trucco MI, Pasantes JJ: Cytogenetics in Brachidontes rodriguezi d’Orb (Bivalvia, Mytilidae). Chromosome Res 7:49–55 (1999).
Vlahoviček K, Kajan L, Pongor S: DNA analysis servers: plot.it, bend.it, model.it and IS. Nucleic Acids Res 13:3686–3687 (2003).
Wang Y, Guo X: Chromosomal rearrangement in Pectinidae revealed by rRNA loci and implications for bivalve evolution. Biol Bull 207:247–256 (2004).
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