Adult males and females of the grasshopper Eyprepocnemis plorans from a Greek population were analysed by C-banding, silver impregnation and double FISH for two DNA probes, i.e. ribosomal DNA (rDNA) and a 180-bp tandem repeat DNA (satDNA). This population shows characteristics of rDNA location in A chromosomes that are intermediate between those previously reported for eastern (Caucasus) and western (Spain and Morocco) populations. The four rDNA clusters revealed by FISH in chromosomes X, 9, 10 and 11 in Greek specimens imply two more than the two observed in chromosomes 9 and 11 in the Caucasus, but less than the 12 observed in all chromosomes in Morocco. Remarkably, the X chromosome bears one of the new rDNA locations in Greece with respect to the Caucasus, but it appears to be inactive, in contrast to X chromosomes in western populations, which are usually active. B chromosomes were very frequent in the Greek population, and three variants differing in size were observed, all of these being largely composed of rDNA, with the exception of a small pericentromeric satDNA cluster. The high B frequency suggests that B chromosomes in this population might behave parasitically, in resemblance to Bs in western populations.

Bakkali M, Camacho JPM: The B chromosome polymorphism of the grasshopper Eyprepocnemis plorans in North Africa: III. Mutation rate of B chromosomes. Heredity 92:428–433 (2004).
Bakkali M, Cabrero J, López-León MD, Perfectti F, Camacho JPM: The B chromosome polymorphism of the grasshopper Eyprepocnemis plorans in North Africa. I. B variants and frequency. Heredity 83:428–434 (1999).
Bakkali M, Cabrero J, López-León MD, Perfectti F, Camacho JPM: Population differences in the expression of nucleolus organizer regions in the grasshopper Eyprepocnemis plorans. Protoplasma 217:185–190 (2001).
Cabrero J, Bakkali M, Bugrov A, Warchalowska-Sliwa E, López-León MD, et al: Multiregional origin of B chromosomes in the grasshopper Eyprepocnemis plorans. Chromosoma 112:207–211 (2003a).
Cabrero J, Bugrov A, Warchalowska-Sliwa E, López-León MD, Perfectti F, Camacho JPM: Comparative FISH analysis in five species of Eyprepocnemidine grasshoppers. Heredity 90:377–381 (2003b).
Cabrero J, Perfectti F, Gómez R, Camacho JPM, López-León MD: Population variation in the A chromosome distribution of satellite DNA and ribosomal DNA in the grasshopper Eyprepocnemis plorans. Chromosome Res 11:375–381 (2003c).
Camacho JPM: B Chromosomes in the Eukaryote Genome (Karger, Basel 2004).
Camacho JPM: B chromosomes, in Gregory TR (ed): The Evolution of the Genome, pp 223–286 (Elsevier, San Diego 2005).
Camacho JPM, Carballo AR, Cabrero J: The B-chromosome system of the grasshopper Eyprepocnemis plorans subsp. plorans (Charpentier). Chromosoma 80:163–176 (1980).
Camacho JPM, Cabrero J, Viseras E, López-León MD, Navas-Castillo J, Alché JD: G-banding in two species of grasshopper and its relationship to C, N, and fluorescence banding techniques. Genome 34:638–643 (1991).
Camacho JPM, Shaw MW, López-León MD, Pardo MC, Cabrero J: Population dynamics of a selfish B chromosome neutralized by the standard genome in the grasshopper Eyprepocnemis plorans. Am Nat 149:1030–1050 (1997).
Castro J, Rodriguez S, Pardo BG, Sánchez L, Martínez P: Population analysis of an unusual NOR-site polymorphism in brown trout (Salmo trutta L.). Heredity 86:291–302 (2001).
González-Sánchez M, González-González E, Molina F, Chiavarino AM, Rosato M, Puertas MJ: One gene determines maize B chromosome accumulation by preferential fertilisation; another gene(s) determines their meiotic loss. Heredity 90:122–129 (2003).
Herrera JA, López-León MD, Cabrero J, Shaw MW, Camacho JPM: Evidence for B chromosome drive suppression in the grasshopper Eyprepocnemis plorans. Heredity 76:633–639 (1996).
Jankun M, Martínez P, Pardo BG, Kirtiklis L, Rab P, et al: Ribosomal genes in Coregonid fishes (Coregonus lavaretus, C. albula and C. peled) (Salmonidae): Single and multiple nucleolus organizer regions. Heredity 87:672–679 (2001).
Jiménez MM, Romera F, González-Sánchez M, Puertas MJ: Genetic control of the rate of transmission of rye B chromosomes. 3. Male meiosis and gametogenesis. Heredity 78:636–644 (1997).
López-Fernández C, Mezzanotte R, Gosálvez J: Autosomal, sex and B-chromosomes in Eyprepocnemis plorans (Orthoptera) viewed with restriction endonuclease in situ digestion. Heredity 68:365–372 (1992).
López-León MD, Cabrero J, Pardo MC, Viseras E, Camacho JPM, Santos JL: Generating high variability of B chromosomes in Eyprepocnemis-plorans (grasshopper). Heredity 71:352–362 (1993).
López-León MD, Neves N, Schwarzacher T, Heslop-Harrison JS, Hewitt GM, Camacho JPM: Possible origin of a B chromosome deduced from its DNA composition using double Fish technique. Chromosome Res 2:87–92 (1994).
López-León MD, Vazquez P, Hewitt GM, Camacho JPM: Cloning and sequence analysis of an extremely homogeneous tandemly repeated DNA in the grasshopper Eyprepocnemis plorans. Heredity 75:370–375 (1995).
Nur U, Brett BLH: Genotypes suppressing meiotic drive of a B-chromosome in the mealybug, Pseudococcus obscurus. Genetics 110: 73–92 (1985).
Riera L, Petitpierre E, Juan C, Cabrero J, Camacho JPM: Evolutionary dynamics of a B chromosome invasion in island populations of the grasshopper Eyprepocnemis plorans. J Evol Biol 17:716–719 (2004).
Romera F, Jiménez MM, Puertas MJ: Genetic control of the rate of transmission of rye B chromosomes. 1. Effects in 2Bx0B crosses. Heredity 66:61–65 (1991).
Rufas JS, Iturra P, DeSouza W, Esponda P: Simple silver staining procedures for the location of nucleolus and nucleolar organizer under light and electron microscopy. Arch Biol 93:267–274 (1982).
Schubert I, Wobus U: In situ hybridization confirms jumping nucleolus organizing regions in Allium. Chromosoma 92:143–148 (1985).
Shaw MW, Hewitt GM: The genetic control of meiotic drive acting on the B chromosome of Myrmeleotettix maculatus (Orthoptera, Acrididae). Heredity 54:187–194 (1985).
Zurita S, Cabrero J, López-León MD, Camacho JPM: Polymorphism regeneration for a neutralized selfish B chromosome. Evolution 52:274–277 (1998).
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