The mammalian ATP2A2 gene encodes a P-type cation pump located in the sarcoplasmic or endoplasmic reticula of muscle cells. We isolated one bacterial artificial chromosome (BAC) clone containing the equine ATP2A2 gene and determined the complete coding sequence of this gene. Cloning and characterization of the equine ATP2A2 gene revealed that the equine ATP2A2 gene consists of 20 exons. In total, 32 horses out of 16 breeds were analyzed for single nucleotide polymorphisms (SNPs). A mutation scan for SNPs included ten exons and their flanking introns. We detected in total 17 SNPs, 14 of which were located in introns, one in exon 9 and two in exon 20. In this report we provide the genomic organization and the equine ATP2A2 coding sequence and an association analysis for chronic pastern dermatitis using a sample of South German draft horses.

1.
Gardiner-Garden M, Frommer M: CpG islands in vertebrate genomes. J Mol Biol 20:261–282 (1987).
2.
Geburek F, Ohnesorge B, Deegen E, Doeleke R, Hewicker-Trautwein M: Alterations of epidermal proliferation and cytokeratin expression in skin biopsies from heavy draught horses with chronic pastern dermatitis. Vet Dermatol 16:373–384 (2005).
3.
Gelebart P, Martin V, Enouf J, Papp B: Identification of a new SERCA2 splice variant regulated during monocytic differentiation. Biochem Biophys Res Commun 303:676–684 (2003).
4.
Missiaen L, Wuytack F, Raeymaekers L, De Smedt H, Droogmans G, et al: Ca(2+) extrusion across plasma membrane and Ca(2+) uptake by intracellular stores. Pharm Ther 50:191–232 (1991).
5.
Müller D, Kuiper H, Mömke S, Böneker C, Drögemüller C, et al: Physical mapping of the ATP2A2 gene to equine chromosome 8p14→p12 by FISH and confirmation by linkage and RH mapping. Cytogenet Genome Res 114:94G (2006).
6.
Otsu K, Fujii J, Periasamy M, Difilippantonio M, Uppender M, et al: Chromosome mapping of five human cardiac and skeletal muscle sarcoplasmic reticulum protein genes.Genomics 17:507–509 (1993).
7.
Ruiz-Perez VL, Carter SA, Healy E, Todd C, Rees JL, et al: ATP2A2 mutations in Darier’s disease: variant cutaneous phenotypes are associated with missense mutations, but neuropsychiatric features are independent of mutation class. Hum Mol Genet 8:1621–1630 (1999).
8.
Sakuntabhai A, Ruiz-Perez V, Carter S, Jacobsen N, Burge S, et al: Mutations in ATP2A2, encoding a Ca(2+) pump, cause Darier disease. Nat Genet 21:271–277 (1999).
9.
Wallraf A, Hamann H, Ohnesorge B, Deegen E, Distl O: Population genetic analysis of chronic pastern dermatitis in South German draught horses. Züchtungskunde 76:246–261 (2004).
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