Several quantitative trait loci for beef carcass traits have been mapped to bovine chromosome 5. The objective of this study was to map six candidate genes for these traits by fluoresence in situ hybridization, genetic linkage analysis and radiation hybrid mapping. MYF5 and MYF6 were assigned to 5q13, WIF1 to 5q23 and MMP19 to 5q25. A paralog of MYF5 (putatively MYOG) was assigned to 16q12. A novel microsatellite placed MYF5 and MYF6 10.4 cM from BM6026 and 19.1 cM from BL23 on the genetic linkage map. MYF5 (62.6 cR), WNT10B (319.5 cR), WIF1 (500.8 cR) and MMP19 (701.2 cR) were also integrated into the 5000Rad radiation hybrid map.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 215:403–410 (1990).
Band MR, Larson JH, Rebeiz M, Green CA, Heyen DW, et al: An ordered comparative map of the cattle and human genomes. Genome Res 10:1359–1368 (2000).
Barendse WJ: Assessing lipid metabolism. International patent application number PCT/AU98/00882. International publication No. WO 99/23248 (1999).
Bennett CN, Ross SE, Longo KA, Bajnok L, Hemati N, et al: Regulation of Wnt signaling during adipogenesis. J Biol Chem 277:30998–31004 (2002).
Boehnke M: Multipoint analysis for radiation hybrid mapping. Ann Med 24:383–386 (1992).
Cai L, Taylor JF, Wing RA, Gallagher DS, Woo S-S, Davis SK: Construction and characterization of a bovine bacterial artificial chromosome library. Genomics 29:413–425 (1995).
Casas E, Shackelford SD, Keele JW, Koohmaraie M, Smith TPL, Stone RT: Detection of quantitative trait loci for growth and carcass composition in cattle. J Anim Sci 81:2976–2983 (2003).
Chowdhary BP, Fronicke L, Gustavsson I, Scherthan H: Comparative analysis of the cattle and human genomes: Detection of ZOO-FISH and gene mapping-based chromsomal homologies. Mamm Genome 7:297–302 (1996).
Chowdhary BP, Raudsepp T, Honeycutt D, Owens EK, Piumi F, et al: Construction of a 5000(rad) whole-genome radiation hybrid panel in the horse and generation of a comprehensive and comparative map for ECA11. Mamm Genome 13:89–94 (2002).
Chowdhary BP, Raudsepp T, Kata SR, Goh G, Millon LV, et al: The first-generation whole-genome radiation hybrid map in the horse identifies conserved segments in human and mouse genomes. Genome Res 13:742–751 (2003).
Davis RL, Weintraub J, Lasser AB: Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell 50:987–1000 (1987).
Gaile DP: Development of statistical tools with applications in radiation hybrid and linkage mapping. Ph.D. Dissertation, (Texas A&M University, College Station 2003).
Gill CA, Davis SK, Taylor JF, Cockett NE, Bottema CDK: Construction and characterization of an ovine bacterial artificial chromosome library. Mamm Genome 10:1108–1111 (1999).
Hsieh JC, Kodjabachian L, Rebbert ML, Rattner A, Smallwood PM, et al: A new secreted protein that binds to Wnt proteins and inhibits their activities. Nature 398:431–436 (1999).
Lange K, Boehnke M, Cox DR, Lunetta KL: Statistical methods for polyploid radiation hybrid mapping. Genome Res 5:136–150 (1995).
Li C, Basarab J, Snelling WM, Benkel B, Kneeland J, et al: Identification and fine mapping of quantitative trait loci for backfat on chromosomes 2, 5, 6, 19, 21, and 23 in a commercial line of Bos taurus. J Anim Sci 82:967–972 (2004a).
Li C, Basarab J, Snelling WM, Benkel B, Murdoch B, et al: Assessment of positional candidate genes MYF5 and IGF1 for growth on bovine chromosome 5 in commercial lines of Bos taurus. J Anim Sci 82:1–7 (2004b).
Lincoln SE, Daly MJ, Lander ES: Primer 0.5 computer software. (MIT Center for Genome Research and Whitehead Institute for Biomedical Research, Cambridge, 1991).
Ozawa A, Band MR, Larson JH, Donovan J, Green CA, et al: Comparative organization of cattle chromosome 5 revealed by comparative mapping by annotation and sequence similarity and radiation hybrid mapping. Proc Natl Acad Sci USA 97:4150–4155 (2000).
Rebhan M, Chalifa-Caspi V, Prilusky J, Lancet D: GeneCards: encyclopedia for genes, proteins and diseases. Weizmann Institute of Science, Bioinformatics Unit and Genome Center. (1997).
Ryan AM, Schelling CP, Womack JE, Gallagher DS: Chromosomal assignment of six muscle-specific genes in cattle. Anim Genet 28:84–87 (1997).
Sambrook J, Fritsch T, Maniatis T: Molecular Cloning: a Laboratory Manual. 2nd ed. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989).
Stone RT, Keele JW, Shackelford SD, Kappes SM, Koohmaraie M: A primary screen of the bovine genome for quantitative trait loci affecting carcass and growth traits. J Anim Sci 77:1379–1384 (1999).
Taylor JF, Coutinho LL, Herring KL, Gallagher DS, Brenneman RA, et al: Candidate gene analysis of GH1 for effects on growth and carcass composition of cattle. Anim Genet29:194–201 (1998).
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