Transfer of alien chromosome segments from various Triticeae species into cultivated wheats, commonly referred to as “chromosome engineering”, is currently benefiting from the recent, impressive advancements in molecular genetics, cytogenetics and genomics, which are providing new insights into the genetic and physical organization of even complex plant genomes, such as those of the Triticeae. The powerful analytical tools presently available are making the assessment of desired genotypes in the course of chromosome engineering far more precise and effective than in the past, thus giving this transfer strategy renewed and increased potential for meaningful practical achievements. Examples are given here of the application of such tools to the engineering of the durum wheat genome with small alien segments containing genes with beneficial impact on disease resistance and quality traits.   

1.
Ahn S, Anderson JA, Sorrells ME, Tanksley SD: Homoeologous relationships of rice, wheat and maize chromosomes. Mol Gen Genet 241:483–490 (1993).
2.
Anamthawat-Jónsson K, Reader SM: Pre-annealing of total genomic DNA probes for simultaneous genomic in situ hybridization. Genome 38:814–816 (1995).
3.
Bennetzen JL: Higher throughput comparative genomics of wheat and related cereals, in Pogna NE, Romanò M, Pogna EA, Galterio G (eds): Proceedings of the 10th International Wheat Genetics Symposium, Paestum, Italy, vol 1, pp 215–220 (SIMI, Rome 2003).
4.
Bennetzen JL, Ramakrishna W: Numerous small rearrangements of gene content, order and orientation differentiate grass genomes. Plant Mol Biol 48:821–827 (2002).
5.
Biagetti M, Vitellozzi F, Ceoloni C: Physical mapping of the wheat-Aegilops longissima breakpoints in mildew resistant recombinant lines using FISH with highly repeated and low-copy DNA probes. Genome 42:1013–1019 (1999).
6.
Braun HJ, Payne TS, Morgounov AI, van Ginkel M, Rajaram S: The challenge: one billion tons of wheat by 2020, in Slinkard AE (ed): Proceedings of the 9th International Wheat Genetics Symposium, Saskatoon, Saskatchewan, Canada, vol 1, pp 33–40 (University Extension Press, University of Saskatchewan 1998).
7.
Carozza R, Pagnotta MA, Ceoloni C: Cytogenetic characterization of chromosomally engineered wheat chromosomes by FISH-based physical maps, molecular linkage maps and meiotic pairing analysis, in Proceedings of the 4th European Cytogenetic Conference, Bologna, Italy, 6–9 September 2003. Annal Génétique 46:301 (2003).
8.
Ceoloni C: Current methods of chromosome engineering in wheat, in Sutka J, Worland AJ (eds): EWAC (European Wheat Aneuploid Cooperative) Newsletter 1987, pp 95–109 (1987).
9.
Ceoloni C, Del Signore G, Pasquini M, Testa A: Transfer of mildew resistance from Triticum longissimum into wheat by induced homoeologous recombination, in Miller TE, Koebner RMD (eds): Proceedings of the 7th International Wheat Genetics Symposium, Cambridge, UK, pp 221–226 (Institute Plant Science Research, Cambridge 1988).
10.
Ceoloni C, Del Signore G, Ercoli L, Donini P: Locating the alien chromatin segment in common wheat-Aegilops longissima mildew resistant transfers. Hereditas 116:239–245 (1992).
11.
Ceoloni C, Ciaffi M, Lafiandra D, Giorgi B: Chromosome engineering as a means of transferring 1D storage protein genes from common to durum wheat, in Li ZS, Xin ZY (eds): Proceedings of the 8th International Wheat Genetics Symposium, Beijing, China, 1993, pp 159–163 (China Agricultural Scientech Press, Beijing 1995).
12.
Ceoloni C, Biagetti M, Ciaffi M, Forte P, Pasquini M: Wheat chromosome engineering at the 4× level: the potential of different alien gene transfers into durum wheat. Euphytica 89:87–97 (1996).
13.
Ceoloni C, Vitellozzi F, Forte P, Basili F, Biagetti M, Bitti A, Delre V: Wheat chromosome engineering in the light of advanced genetic and cytogenetic marker-mediated approaches, in Lelley T (ed): Current Topics in Plant Cytogenetics Related to Plant Improvement, pp 43–53 (WUV-Universitätsverlag, Wien 1998).
14.
Ceoloni C, Forte P, Ciaffi M, Nenno M, Bitti A, De Vita P, D’Egidio MG: Chromosomally engineered durum wheat: the potential of alien gene introgressions affecting disease resistance and quality, in Proceedings of the Seminar on Durum Wheat Improvement in the Mediterranean Region: New Challenges, Zaragoza (Spain), 12–14 April 2000. Options Méditerranéennes A-40:363–371 (2000).
15.
Ceoloni C, Margiotta B, Colaprico G, D’Egidio MG, Carozza R, Lafiandra D: Introgression of D-genome associated gluten protein genes into durum wheat, in Pogna NE, Romanò M, Pogna EA, Galterio G (eds): Proceedings of the 10th International Wheat Genetics Symposium, Paestum, vol 2, pp 1320–1322 (S.I.M.I., Rome 2003).
16.
Ceoloni C, Pasquini M, Simeone R: The cytogenetic contribution to the analysis and manipulation of the durum wheat genome, in Royo C, Nachit MN, Di Fonzo N, Araus JL, Pfeiffer WH, Slafer GA (eds): Durum Wheat Breeding: Current Approaches and Future Strategies (The Haworth Press, New York, in press 2004).
17.
Chao S, Sharp PJ, Worland AJ, Warham EJ, Koebner RMD, Gale MD: RFLP-based genetic maps of wheat homoeologous group 7 chromosomes. Theor Appl Genet 78:495–504 (1989).
18.
de Jong JH, Franz P, Zabel P: High resolution FISH in plants – techniques and applications. Trends Plant Sci 4:258–263 (1999).
19.
Devos KM, Gale MD: Comparative genetics in the grasses. Plant Mol Biol 35:3–15 (1997).
20.
Devos KM, Gale MD: Genome relationships: the grass model in current research. Plant Cell 12:637–646 (2000).
21.
Devos KM, Atkinson MD, Chinoy CN, Liu C, Gale MD: RLFP based genetic map of the homoeologous group 3 chromosomes of wheat and rye. Theor Appl Genet 83:931–939 (1992).
22.
Devos KM, Millan T, Gale MD: Comparative RFLP maps of the homoeologous group-2 chromosomes of wheat, rye and barley. Theor Appl Genet 85:784–792 (1993).
23.
Devos KM, Dubcovsky J, Dvorak J, Chinoy CN, Gale MD: Structural evolution of wheat chromosomes 4A, 5A and 7B and its impact on recombination. Theor Appl Genet 91:282–288 (1995).
24.
Dvorak J, Akhunov ED, Akhunov AR, Luo MC, Linkiewicz AM, Dubcovsky J, Hummel D, Lazo G, Chao S, Anderson OD, David J, Qi LL, Echalier B, Gill BS, Miftahudin, Gustafson JP, et al: New insights into the organization and evolution of wheat genomes, in Pogna NE, Romanò M, Pogna EA, Galterio G (eds): Proceedings of the 10th International Wheat Genetics Symposium, Paestum, Italy, vol 1, pp 247–253 (S.I.M.I., Rome 2003).
25.
Eizenga GC: Locating the Agropyron segment in wheat-Agropyron “transfer No. 12”. Genome 29:365–366 (1987).
26.
Feldman M: Cytogenetic and molecular approaches to alien gene transfer in wheat, in Miller TE, Koebner RMD (eds): Proceedings of the 7th International Wheat Genetics Symposium, Cambridge, UK, pp 23–32 (Institute of Plant Science Research, Cambridge 1988).
27.
Feldman M: Cytogenetic activity and mode of action of the pairing homoeologous (Ph1) gene of wheat. Crop Sci 33:894–897 (1993).
28.
Feldman M, Sears ER: The wild gene resources of wheat. Sci Am 244:98–109 (1981).
29.
Feuillet C, Keller B: Comparative genomics in the grass family: molecular characterization of grass genome structure and evolution. Ann Bot 89:3–10 (2002).
30.
Gennaro A, Borrelli GM, D’Egidio MG, De Vita P, Ravaglia S, Ceoloni C: A chromosomally engineered durum wheat-Thinopyrum ponticum recombinant line with novel and promising attributes for varietal development, in Pogna NE, Romanò M, Pogna EA, Galterio G (eds): Proceedings of the 10th International Wheat Genetics Symposium, Paestum, Italy, vol 2, pp 881–883 (S.I.M.I., Rome 2003).
31.
Gill KS, Gill BS, Endo TR, Mukai Y: Fine physical mapping of Ph1, a chromosome pairing regulator gene in polyploid wheat. Genetics 134:1231–1236 (1993).
32.
Giorgi B: Origin, behaviour and utilization of a Ph1 mutant of durum wheat, Triticum turgidum (L.) var. durum, in Sakamoto S (ed): Proceedings of the 6th International Wheat Genetics Symposium, Kyoto, Japan, pp 1033–1040 (Plant Germplasm Institute, Kyoto University 1983).
33.
Heslop-Harrison JS: Comparative genome organization in plants: from sequence and markers to chromatin and chromosomes. Plant Cell 12:617–635 (2000).
34.
Khush GS: Green revolution: preparing for the 21st century. Genome 42:646–655 (1999).
35.
Knott DR: Mutation of a gene for yellow pigment linked to Lr19 in wheat. Can J Genet Cytol 22:651–654 (1980).
36.
Kohli A, Twyman RM, Abrances R, Wegel E, Stoger E, Christou P: Transgene integration, organization and interaction in plants. Plant Mol Biol 52:247–258 (2003).
37.
Kosambi DD: The estimation of map distances from recombination values. Ann Eugenics 12:172–175 (1944).
38.
Laurie DA, Devos KM: Trends in comparative genetics and their potential impacts on wheat and barley research. Plant Mol Biol 48:729–740 (2002).
39.
Lukaszewski AJ: Physical distribution of translocation breakpoints in homoeologous recombinants induced by the absence of the Ph1 gene in wheat and triticale. Theor Appl Genet 90:714–719 (1995).
40.
Lukaszewski AJ: Experimental designs in induced homoeologous recombination in wheat, in Lelley T (ed): Current Topics in Plant Cytogenetics Related to Plant Improvement, pp 54–62 (WUV-Universitätsverlag Wien 1998).
41.
Lukaszewski AJ: Manipulation of crossing over in wheat, in Pogna NE, Romanò M, Pogna EA, Galterio G (eds): Proceedings of the 10th International Wheat Genetics Symposium, Paestum, Italy, vol 1, pp 73–76 (S.I.M.I., Rome, 2003).
42.
Micali S, Forte P, Bitti A, D’Ovidio R, Ceoloni C: Chromosome engineering as a tool for effectively introgressing multiple useful genes from alien Triticeae into durum wheat, in Pogna NE, Romanò M, Pogna EA, Galterio G (eds): Proceedings of the 10th International Wheat Genetics Symposium, Paestum, Italy, vol 2, pp 896–898 (S.I.M.I., Rome 2003).
43.
Moore G, Devos KM, Wang Z, Gale MD: Cereal genome evolution: grasses, line up and form a circle. Curr Biol 5:737–739 (1995).
44.
Naranjo T: Chromosome structure of durum wheat. Theor Appl Genet 79:397–400 (1990).
45.
Naranjo T: Chromosome structure of Triticum longissimum relative to wheat. Theor Appl Genet 91:105–109 (1995).
46.
Naranjo T, Fernández-Rueda P: Homoeology of rye chromosome arms to wheat. Theor Appl Genet 82:577–586 (1991).
47.
Naranjo T, Roca A, Giraldez R, Goicoechea PG: Chromosome pairing in hybrids of ph1b mutant wheat with rye. Genome 30:639–646 (1988).
48.
Riley R: The diploidization of polyploid wheat. Heredity 15:407–429 (1960).
49.
Sears ER: Homoeologous chromosomes in Triticum aestivum. Genetics 37:624 (1952).
50.
Sears ER: The aneuploids of common wheat. Univ MO Agric Exp Stn Res Bull 572:1–59 (1954).
51.
Sears ER: Nullisomic-tetrasomic combinations in hexaploid wheat, in Riley R, Lewis KR (eds): Chromosome Manipulations and Plant Genetics, pp 29–45 (Oliver and Boyd, Edinburgh 1966).
52.
Sears ER: Chromosome engineering in wheat. Proc Stadler Genet Symp 4:23–38 (1972).
53.
Sears ER: Agropyron-wheat transfers obtained by homoeologous pairing, in Sears ER, Sears LMS (eds): Proceedings of the 4th International Wheat Genetics Symposium, Columbia, Missouri, pp 191–199 (Agricultural Experiment Station, College of Agriculture, University of Missouri, Columbia 1973).
54.
Sears ER: Genetic control of chromosome pairing in wheat. Ann Rev Genet 10:31–51 (1976).
55.
Sears ER: An induced mutant with homoeologous pairing in common wheat. Can J Genet Cytol 19:585–593 (1977).
56.
Sears ER: Analysis of wheat-Agropyron recombinant chromosomes, in Sanchez-Monge, Garcia-Olmedo F (eds): Interspecific Hybridization in Plant Breeding, Proceedings of the 8th EUCARPIA Congress Madrid, Spain, 1977, pp 63–72 (Escuela Técnica Superior de Ingenieros Agrónomos, Ciudad Universitaria, Madrid 1978).
57.
Sears ER: Transfer of alien genetic material to wheat, in Evans LT, Peacock WJ (eds): Wheat Science – Today and Tomorrow, pp 75–89 (Cambridge University Press, Cambridge 1981).
58.
Sears ER: The transfer to wheat of interstitial segments of alien chromosomes, in Sakamoto S (ed): Proceedings of the 6th International Wheat Genetics Symposium, Kyoto, Japan, pp 5–12 (Plant Germplasm Inst., Kyoto University 1983).
59.
Van Deynze AE, Sorrells ME, Park WD, Ayres NM, Fu H, Cartinhour SW, Paul E, McCouch SR: Anchor probes for comparative mapping of grass genera. Theor Appl Genet 97:356–369 (1998).
60.
Vitellozzi F, Ciaffi M, Dominici L, Ceoloni C: Isolation of a chromosomally engineered durum wheat line carrying the common wheat Glu-D1d allele. Agronomie 17:413–417 (1997).
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