Abstract
We describe how we are furthering our understanding of meiosis in rye (Secale cereale L.) using a combination of cytogenetic and molecular biological approaches. Fluorescent in situ hybridisation, electron microscopy of synaptonemal complexes, sequencing of meiosis-specific genes, and the immunolocalisation of recombinogenic proteins are being combined to build up phenotypic “identikits” of wild type, asynaptic mutants sy1 and sy9, and desynaptic mutant sy10. From this information, we review the status of our current understanding of the genetic control of meiosis in rye, and consider strategies for determining more precisely the interrelationships between meiosis-specific genes and their products.
References
1.
Abranches R, Beven AF, Aragon-Alcaide L, Shaw PJ: Transcription sites are not correlated with chromosome domains in wheat nuclei. J Cell Biol 143:5–12 (1998).
2.
Ade J, Belzile F, Philippe H, Doutriaux M-P: Four mismatch repair paralogues coexist in Arabidopsis thaliana: AtMSH2, AtMSH3, AtMSH6-1 and AtMSH6-2. Mol Gen Genet 262:239–249 (1999).
3.
Anderson LK, Stack SM: Meiotic recombination in plants. Curr Genom 3:507–525 (2002).
4.
Anderson LK, Offenberg HH, Verkuijlen WMHC, Heyting C: RecA-like proteins are components of early meiotic nodules in lily. Proc Natl Acad Sci USA 94:6868–6873 (1997).
5.
Aragon-Alcaide L, Reader S, Miller T, Moore G: Centromeric behaviour in wheat with high and low homoeologous chromosomal pairing. Chromosoma 106:327–333 (1997).
6.
Bass HW, Marshall WF, Sedat JW, Agard DA, Cande WZ: Telomeres cluster de novo before the initiation of synapsis: A three dimensional spatial analysis of telomere positions before and during meiotic prophase. J Cell Biol 137:5–18 (1997).
7.
Bass HW, Riera-Lizarazu O, Ananiev EV, Bordoli SJ, Rines HW, Phillips RL, Sedat JW, Agard DA, Cande WZ: Evidence for the coincident initiation of homolog pairing and synapsis during telomere-clustering (bouquet) stage of meiotic prophase. J Cell Sci 113:1033–1042 (2000).
8.
Bogdanov YF: Variation and evolution of meiosis. Russ J Genet 39:453–473 (2003).
9.
Bogdanov YF, Fedotova YS, Sosnikhina SP, Smirnov VG, Dadashev SY, Mikhailova EI, de Jong JH: Bar and thorn-like abnormalities in synaptonemal complex of a mutant rye, Secale cereale. Genome 44:284–288 (1998).
10.
Cao L, Alani E, Kleckner N: A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae. Cell 61:1089–1101 (1990).
11.
Caryl AP, Armstrong SJ, Jones GH, Franklin FCH: A homologue of the yeast HOP1 gene is inactivated in Arabidopsis meiotic mutant asy1. Chromosoma 109:62–71 (2000).
12.
Fedotova YS, Bogdanov YF, Gadzhiyeva SA, Sosnikhina SP, Smirnov VG, Mikhailova EI: Meiotic mutants of rye Secale cereale L. II. The nonhomologous synapsis in desynaptic mutants sy7 and sy10. Theor Appl Genet 88:1029–1036 (1994).
13.
Feng Q, Zhang Y, Hao P, Wang S, Fu G, et al: Sequence analysis of rice chromosome 4. Nature 420:316–320 (2002).
14.
Goff SA, Ricke D, Lan TH, Presting G, Wang R, et al: A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science 296:92–100 (2002).
15.
Golubovskaya IN, Grebennikova ZK, Auger DL, Sheridan WF: The maize desynaptic 1 mutation disrupts meiotic chromosome synapsis. Dev Genet 21:146–159 (1997).
16.
Hartung F, Puchta H: Molecular characterisation of two paralogous SPO11 homologues in Arabidopsis thaliana. Nucleic Acids Res 28:1548–1554 (2000).
17.
Heyting C: Synaptonemal complexes: structure and function. Curr Opin Cell Biol 8:389–396 (1996).
18.
Hollingsworth NM, Byers B: HOP1: a yeast meiotic pairing gene. Genetics 121:445–462 (1989).
19.
Jenkins G, Okumus A: Indiscriminate synapsis in achiasmate Allium fistulosum L. (Liliaceae). J Cell Sci 103:415–422 (1992).
20.
Jones GH, Armstrong SJ, Caryl AP, Franklin FCH: Meiotic chromosome synapsis and recombination in Arabidopsis thaliana; an integration of cytological and molecular approaches. Chromosome Res 11:205–215 (2003).
21.
Keeney S: Mechanism and control of meiotic recombination initiation. Curr Topics Dev Biol 52:1–53 (2001).
22.
Kleckner N: Meiosis: How could it work? Proc Natl Acad Sci USA 93:8167–8174 (1996).
23.
Korzun V, Boerner A, Siebert R, Malyshev S, Hilpert M, Kunze R, Puchta H: Chromosomal location and genetic mapping of the mismatch repair gene homologs MSH2, MSH3, and MSH6 in rye and wheat. Genome 42:1255–1257 (1999).
24.
Martinez-Perez E, Shaw P, Reader S, Aragon-Alcaide L, Miller T, Moore G: Homologous chromosome pairing in wheat. J Cell Sci 112:1761–1769 (1999).
25.
Martinez-Perez E, Shaw PJ, Moore G: Polyploidy induces centromere association. J Cell Biol 148:233–238 (2000).
26.
Mikhailova EI, Sosnikhina SP, Kirillova GA, Tikholiz OA, Smirnov VG, Jones RN, Jenkins G: Nuclear dispositions of subtelomeric and percentromeric chromosomal domains during meiosis in asynaptic mutants of rye (Secale cereale L.). J Cell Sci 114:1875–1882 (2001).
27.
Moore G: To pair or not to pair, chromosome pairing and evolution. Curr Opin Plant Biol 1:116–122 (1998).
28.
Roeder GS: Meiotic chromosomes: it takes two to tango. Genes Devel 11:2600–2621 (1997).
29.
Sasaki T, Matsumoto T, Yamamoto K, Sakata K, Baba T, et al: The genome sequence and structure of rice chromosome 1. Nature 420:312–316 (2002).
30.
Scherthan H, Weich S, Schwegler H, Heyting C, Harle M, Cremer T: Centromere and telomere movements during early meiotic prophase of mouse and man are associated with the onset of chromosome pairing. J Cell Biol 134:1109–1125 (1996).
31.
Shaw P, Moore G: Meiosis: Vive la difference! Curr Opin Plant Biol 1:458–462 (1998).
32.
Sosnikhina SP, Fedotova YS, Smirnov VG, Mikhailova EI, Kolomiets OL, Bogdanov YF: Meiotic mutants of rye Secale cereale L. I. Synaptic mutant sy-1. Theor Appl Genet 84:979–985 (1992a).
33.
Sosnikhina SP, Smirnov VG, Mikhailova EI, Egorova LF: The mutation of partial asynapsis in rye. Genetika 28:128–136 (1992b).
34.
Sosnikhina SP, Fedotova YS, Smirnov VG, Mikhailova EI, Bogdanov YF: The study of genetic control of meiosis in rye. Russ J Genet 30:909–920 (1994a).
35.
Sosnikhina SP, Smirnov VG, Mikhailova EI, Egorova LF: Disturbance of homologous synapsis in meiotic mutants of diploid rye. Genetika 30:1029–1036 (1994b).
36.
Sosnikhina SP, Smirnov VG, Mikhailova EI, Egorova LF: Homologous synapsis distortion in meiotic mutants of diploid rye. Russ J Genet 30:431–436 (1994c).
37.
Sosnikhina SP, Kirillova GA, Mikhailova EI, Smirnov VG, Fedotova YS, Bogdanov YF: Genetic control of synapsis in rye Secale cereale L.: The sy9 asynaptic gene. Russ J Genet 34:1278–1285 (1998).
38.
Sosnikhina SP, Kirillova GA, Mikhailova EI, Tikholiz OA, Smirnov VG, Fedotova YS, Kolomiets OL, Bogdanov YF: Genetic control of chromosome synapsis at meiosis in rye Secale cereale L.: The sy19 gene controlling heterologous synapsis. Russ J Genet 37:71–79 (2001).
39.
Sosnikhina SP, Kirillova GA, Tikholiz OA, Mikhailova EI, Priyatkina SN, Smirnov VG: Genetic analysis of mutation sy2 which causes nonhomologous meiotic synapsis in chromosomes of diploid rye Secale cereale L. Russ Genet 38:269–276 (2002a).
40.
Sosnikhina SP, Kirillova GA, Tikholiz OA, Mikhailova EI, Smirnov VG, Fedotova YS, Mazurova TF, Bogdanov YF: The expression of mutation sy2 causing nonhomologous synapsis in meiosis of diploid rye Secale cereale L. Russ J Genet 38:156–164 (2002b).
41.
Sym M, Engebrecht JA, Roeder GS: ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis. Cell 72:365–378 (1993).
42.
The Arabidopsis Genome Initiative: Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815 (2000).
43.
Timopheeva LP, Golubovskaya IN: A new type of desynaptic gene in maize revealed by the microspreading method of synaptonemal compexes. Cytologia 33:3–8 (1991).
44.
Trelles-Sticken E, Loidl J, Scherthan H: Bouquet formation in budding yeast: initiation of recombination is not required for meiotic telomere clustering. J Cell Sci 112:651–658 (1999).
45.
Vershinin AV, Schwarzacher T, Heslop-Harrison JS: The large-scale genomic organisation of repetitive DNA families at telomeres of rye chromosomes. Plant Cell 7:1823–1833 (1995).
46.
Weiner BM, Kleckner N: Chromosome pairing via multiple interstitial interactions before and during meiosis in yeast. Cell 77:977–991 (1994).
47.
Yu J, Hu S, Wang J, Wong GK, Li S, et al: A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science 296:79–92 (2002).
48.
Zickler D, Kleckner N: The leptotene-zygotene transition of meiosis. Annu Rev Genet 32:619–697 (1998).
49.
Zickler D, Kleckner N: Meiotic chromosomes: integrating structure and function. Annu Rev Genet 33:603–754 (1999).
© 2005 S. Karger AG, Basel
2005
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