The molecular structure of the exceptional telomeres of six plant species belonging to the order Asparagales and two species of the order Liliales was analyzed using Southern blot and fluorescence in situ hybridization. Three different situations were found, namely: i) In the two Liliales species, Tulipa australis (Liliaceae) and Merendera montana (Colchicaceae), the chromosome ends display hybridization signals with oligonucleotides resembling telomere repeats of both plants (TTTAGGG)n and vertebrates (TTAGGG)n. ii) Asparagales species such as Phormium tenax (Hemerocallidaceae), Muscari comosum (Hyacinthaceae), Narcissus jonquilla (Amaryllidaceae) and Allium sativum (Alliaceae) lack both the plant telomere repeats and the vertebrate telomere repeats. iii) Two other Asparagales species, Aloe vera (Asphodelaceae) and an Iris hybrid (Iridaceae), display positive hybridization with the vertebrate telomere repeats but not with the plant telomere repeats. Southern blot hybridization revealed concurring results. On this basis, the composition of the telomere structure in this plant group is discussed.   

Adams SP, Leitch IJ, Bennett MD, Leitch AR: Aloe L. – a second plant family without (TTTAGGG)n telomeres. Chromosoma 109:201–205 (2000).
Adams SP, Hartman TPV, Lim KY, Chase MW, Bennett MD, Leitch IJ, Leitch AR: Loss and recovery of Arabidopsis-type telomere repeat sequences 5′-(TTTAGGG)n-3′ in the evolution of a major radiation of flowering plants. Proc R Soc Lond B 268:1541–1546 (2001).
Adegoke JA, Arnason U, Widegren B: Sequence organization and evolution, in all extant whalebone whales, of a satellite DNA with terminal chromosome localization. Chromosoma 102:382–388 (1993).
Biessmann H, Zurovcova M, Yao JG, Lozovskaya E, Walter MF: A telomeric satellite in Drosophila virilis and its sibling species. Chromosoma 109:372–380 (2000).
Blackburn EH, Greider CW: Telomeres (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1995).
Chase MW, Duvall MR, Hills HG, Conran JG, Cox AV, Eguiarte LE, Hartwell J, Fay MF, Caddix LR, Cameron KM, Hoot S: Molecular phylogenetics of Lilianae, in Rudall PJ, Cribb PJ, Cutler DF, Humphries CJ (eds): Monocotyledons: Systematics and Evolution, Vol 1, pp 109–137 (Royal Botanic Gardens, Kew 1995a).
Chase MW, Stevenson DW, Wilkin P, Rudall PJ: Monocots sytematics: a combined analysis, in Rudall PJ, Cribb PJ, Cutler DF, Humphries CJ (eds): Monocotyledons: Systematics and Evolution, Vol 2, pp 685–730 (Royal Botanic Gardens, Kew 1995b).
Chase MW, Soltis DE, Soltis PS, Rudall PJ, Fay MF, Hahn WJ, Sullivan S, Josepj J, Molvray M, Kores PJ, Givnish TJ, Sytsma KJ, Pires JC: Higher-level systematics of the monocotyledons: an assessment of current knowledge and a new classification, in Proceedings of Monocot II: The Second International Symposium on the Comparative Biology of the Monocotyledons, Sydney, Australia (CSIRO Press, Sydney 1999).
Cronquist A: The Evolution and Classification of Flowering Plants, 2nd ed (New York Botanical Garden, Brown, NY 1988).
Cuñado N, De la Herrán R, Santos JL, Ruiz Rejón C, Garrido-Ramos MA, Ruiz Rejón M: The evolution of the ribosomal loci in the subgenus Leopoldia of the genus Muscari (Hyacinthaceae). Plant Syst Evol 221:245–252 (2000).
Cuñado N, Sánchez-Morán E, Barrios J, Santos JL: Searching for telomeric sequences in two Allium species. Genome 44:640–643 (2001).
Dahlgren RMT, Clifford HT, Yeo PF: The Families of Monocotyledons: Structure, Evolution and Taxonomy (Springer-Verlag, Berlin 1985).
De la Herrán R, Robles F, Cuñado N, Santos JL, Ruiz Rejón M, Garrido-Ramos MA, Ruiz Rejón C: A heterochromatic satellite DNA is highly amplified in a single chromosome of Muscari (Hyacinthaceae). Chromosoma 110:197–202 (2001).
Dellaporta SL, Wood J, Hicks JB: A plant DNA minipreparation. Version II. Plant Mol Biol Rep 1:19–21 (1983).
Duvall MR, Clegg MT, Chase MW, Clark WD, Kress WJ, Hills HG, Eguiarte LE, Smith JF, Gaut BS, Zimmer EA, Learn GH Jr: Phylogenetic hypotheses for the monocotyledons constructed from rbcl. Ann Missouri Botanical Garden 80:607–619 (1993).
Eickbush TH: Telomerases and retro-transposons: which came first? Science 277:911–912 (1997).
Fanning TG: Origin and evolution of a major feline satellite DNA. J Mol Biol 197:627–634 (1987).
Fitzgerald MS, Shakirov EV, Hood EE, McKnight TD, Shippen DE: Different modes of de novo telomere formation by plant telomerases. Plant J 26:77–87 (2001).
Garrido-Ramos M, De la Herrán R, Ruiz Rejón C, Ruiz Rejón M: A satellite DNA of the Sparidae family (Pisces, Perciformes) associated with telomeric sequences. Cytogenet Cell Genet 83:3–9 (1998).
Gerlach WL, Bedbrook JR: Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885 (1979).
Henderson E: Telomere DNA structure, in Blackburn EH, Greider CW (eds): Telomeres, pp 11–34 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1995).
Huber H: Die Samenmerkmale und Verwandtschaftsverhältnisse der Liliiflorae. Mitteilungen der Botanischen Staatssammlung München 8:219–538 (1969).
Kipling D: The Telomere (Oxford University Press, Oxford 1995).
López CC, Nielsen L, Edström JE: Terminal long tandem repeats in chromosomes from Chironomus pallidivittatus. Mol Cell Biol 16:3285–3290 (1996).
Martínez JL, Edström JE, Morcillo G, Díez JL: Telomeres of Chironomus thummi are characterized by different subfamilies of complex DNA repeats. Chromosoma 110:221–227 (2001).
Mason JM, Biessmann H: The unusual telomeres of Drosophila. Trends Genet 11:58–62 (1995).
Pardue ML, Danilevskaya ON, Lowenhaupt K, Slot F, Traverse KL: Drosophila telomeres: new views on chromosome evolution. Trends Genet 12:48–52 (1996).
Pich U, Schubert I: Terminal heterochromatin and alternative telomeric sequences in Allium cepa. Chromosome Res 6:315–321 (1998).
Pich U, Fuchs J, Schubert I: How do Alliaceae stabilize their chromosome ends in the absence of TTTAGGG sequences? Chromosome Res 4:207–213 (1996a).
Pich U, Fritsch R, Schubert I: Closely related Allium species (Alliaceae) share a very similar satellite sequence. Plant Syst Evol 202:255–264 (1996b).
Richards EJ: Plant telomeres, in Blackburn EH, Greider CW (eds): Telomeres, pp 371–387 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1995).
Richards EJ, Ausubel FM: Isolation of a higher eukaryotic telomere from Arabidopsis thaliana. Cell 53:127–136 (1988).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning, a Laboratory Guide, 2nd ed (Cold Spring Harbor Laboratory Press, New York 1989).
Sýkorova E, Lim KY, Kunická Z, Chase MW, Bennet MD, Fajkus J, Leitch AR: Telomere variability in the monocotyledonous plant order Asparagales. Proc R Soc Lond B 270:1893–1904 (2003).
Thorne RF: A phylogenetic classification of the Angiospermae. Evol Biol 9:35–106 (1976).
Tsujimoto H, Usami N, Hasegawa K, Yamada T, Nagaki K, Sasakuma T: De novo synthesis of telomere sequences at the healed breakpoints of wheat deletion chromosomes. Mol Gen Genet 262:851–856 (1999).
Weiss H, Scherthan H: Aloe spp-plants with vertebrate-like telomeric sequences. Chromosome Res 10:155–164 (2002).
Weiss-Schneeweiss H, Riha K, Jang CG, Puizina J, Scherthan H, Schweizer D: Chromosome termini of the monocot plant Othocallis siberica are maintained by telomerase, which specifically synthesizes vertebrate-type telomere sequences. Plant J 37:484–493 (2004).
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.