To investigate the karyotypic relationships between Chinese muntjac (Muntiacus reevesi), forest musk deer (Moschus berezovskii) and gayal (Bos frontalis), a complete set of Chinese muntjac chromosome-specific painting probes has been assigned to G-banded chromosomes of these three species. Sixteen autosomal probes (i.e. 6–10, 12–22) of the Chinese muntjac each delineated one pair of conserved segments in the forest musk deer and gayal, respectively. The remaining six autosomal probes (1–5, and 11) each delineated two to five pairs of conserved segments. In total, the 22 autosomal painting probes of Chinese muntjac delineated 33 and 34 conserved chromosomal segments in the genomes of forest musk deer and gayal, respectively. The combined analysis of comparative chromosome painting and G-band comparison reveals that most interspecific homologous segments show a high degree of conservation in G-banding patterns. Eleven chromosome fissions and five chromosome fusions differentiate the karyotypes of Chinese muntjac and forest musk deer; twelve chromosome fissions and six fusions are required to convert the Chinese muntjac karyotype to that of gayal; one chromosome fission and one fusion separate the forest musk deer and gayal. The musk deer has retained a highly conserved karyotype that closely resembles the proposed ancestral pecoran karyotype but shares none of the rearrangements characteristic for the Cervidae and Bovidae. Our results substantiate that chromosomes 1–5 and 11 of Chinese muntjac originated through exclusive centromere-to-telomere fusions of ancestral acrocentric chromosomes.   

1.
Bonnet A, Thevenon S, Claro F, Gautier M, Hayes H: Cytogenetic comparison between Vietnamese sika deer and cattle: R-banded karyotypes and FISH mapping. Chromosome Res 9:673–687 (2001).
2.
Chowdhary BP, Frönicke L, Gustavsson I, Scherthan H: Comparative analysis of the cattle and human genomes: detection of Zoo-FISH and gene mapping-based chromosomal homologies. Mamm Genome 7:297–302 (1996).
3.
Chowdhary BP, Raudsepp T, Frönicke L, Scherthan H: Emerging patterns of comparative genome organization in some mammalian species as revealed by Zoo-FISH. Genome Res 8:577–589 (1998).
4.
Ferguson-Smith MA, Yang F, O’Brien PCM: Comparative mapping using chromosome sorting and painting. ILAR J 39:68–76 (1998).
5.
Fontana F, Rubini M: Chromosome evolution in Cervidae. Biosystems 24:157–174 (1990).
6.
Frönicke L, Scherthan H: Zoo-fluorescence in situ hybridization analysis of human and Indian muntjac karyotypes (Muntiacus muntjak vaginalis) reveals satellite DNA clusters at the margins of conserved syntenic segments. Chromosome Res 5:254–261 (1997).
7.
Gallagher DS, Womack JE: Chromosome conservation in the Bovidae. J Hered 83:287–298 (1992).
8.
Gao YT: Chinese musk deer’s classification. Acta Zool Sinica 15:479–488 (1963).
9.
Graphodatsky AS: Conserved and variable elements of mammalian chromosomes, in Halnan CRE (ed): Cytogenetics of Animals, pp 95–124 (CAB International Press, Oxon 1989).
10.
Groves CP, Grubb P: Relationship of living deer, in Wemmer CM (ed): Biology and Management of the Cervidae, pp 21–59 (Smithsonian Institute, Washington 1987).
11.
Groves CP, Wang YX, Grubb P: Taxonomy of musk deer, Genus Moschus (Moschidae, Mammalia). Acta Theriol Sinica 15:181–197 (1995).
12.
Guo JM, Wang JH, Fan H, Deng FM, Zhang ZG: Preliminary study on the karyotype of musk deer (Moschus berezovskii) [in Chinese]. Acta Theriol Sinica 8:73–75 (1988).
13.
Hartmann N, Scherthan H: Characterization of ancestral chromosome fusion points in the Indian muntjac deer. Chromosoma 112:213–220 (2004).
14.
Hassanin A, Douzery EJ: Molecular and morphological phylogenies of Ruminantia and the alternative position of the Moschidae. Syst Biol 52:206–228 (2003).
15.
Hayes H: Chromosome painting with human chromosome-specific DNA libraries reveals the extent and distribution of conserved segments in bovine chromosomes. Cytogenet Cell Genet 71:168–174 (1995).
16.
Hayes H, Di Meo GP, Gautier M, Laurent P, Eggen A, Iannuzzi L: Location by FISH of the 31 Texas nomenclature type I markers to both Q- and R-banded bovine chromosomes. Cytogenet Cell Genet 90:315–320 (2000).
17.
ISCNDB, International system for chromosome nomenclature of domestic bovids, Di Berardino D, Di Meo GP, Gallagher DS, Hayes H, Iannuzzi L (coordinator) (eds): Cytogenet Cell Genet 92:283–299 (2001).
18.
Lan H, Xiong XK, Lin SY, Liu AH, Shi L: Yunnan cattle and gayal’s mtDNA research [in Chinese]. Acta Genet Sin 20:419–425 (1993).
19.
Larkin DM, Wind AED, Rebeiz M, Schweitzer PA, Bachman S, Green C, Wright CL, Campos EJ, Benson LD, Edwards J, Liu L, Osoegawa K, Womack JE, de Jong PJ, Lewin HA: A cattle-human comparative map built with cattle BAC-ends and human genome sequence. Genome Res 13:1966–1972 (2003).
20.
Neitzel H: Chromosome evolution of Cervidae: karyotypic and molecular aspects, in Obe G, Basler A (eds): Cytogenetics – Basic and Applied Aspects, pp 92–112 (Springer-Verlag, Berlin 1987).
21.
Nie L, Shi L, He XD, Zhao YL, Mu WG, Zhang JL: The gayal’s genetics diversity and its genetic structure’s enzyme analysis [in Chinese]. Acta Genet Sin 22:185–191 (1995).
22.
Nie W, Wang J, O’Brien PCM, Fu B, Tian Y, Ferguson-Smith MA, Yang F: The genome phylogeny of domestic cat, red panda and five mustelid species revealed by comparative chromosome painting and G-banding. Chromosome Res 10:209–222 (2002).
23.
Nowak RM: Walker’s Mammals of the World, 6th ed., Vol. II (The Johns Hopkins University Press, Baltimore 1999).
24.
O’Brien SJ, Menotti-Raymond M, Murphy WJ, Nash WG, Wienberg J, Stanyon R, Copeland NG, Jenkins NA, Womack JE, Marshall JA, Graves M: The promise of comparative genomics in mammals. Science 286:458–481 (1999).
25.
Seabright M: The use of proteolytic enzymes for the mapping of structural rearrangements in chromosomes of man. Chromosoma 36:204–210 (1972).
26.
Shi L, Ye YY, Duan XS: Comparative cytogenetic studies on the red muntjac, Chinese muntjac, and their F1 hybrids. Cytogenet Cell Genet 26:22–27 (1980).
27.
Simpson GG: The principles of classification and a classification of mammals. Bull Am Mus Nat Hist 85:1–350 (1945).
28.
Slate J, Stijn TCV, Anderson RM, McEwan KM, Maqbool NJ, Mathias HC, Bixley MJ, Stevens DR, Molenaar AJ, Beever JE, Galloway SM, Tate ML: A deer (Subfamily Cervinae) genetic linkage map and the evolution of ruminant genomes. Genetics 160:1587–1597 (2002).
29.
Su B, Wang YX, Lan H, Wang W, Zhang YP: Phylogenetic study of complete cytochrome b genes in musk deer (genus Moschus) using museum samples. Mol Phylogenet Evol 12:241–249 (1999).
30.
Su B, Wang YX, Wang QS: Mitochondrial DNA sequences imply Anhui musk deer a valid species in genus Moschus. Zool Res 22:169–173 (2001).
31.
Telenius H, Pelmear AH, Tunnacliffe A, Carter NP, Behmel A, Ferguson-Smith MA, Nordenskjold R, Pfranger R, Ponder BAJ: Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes. Genes Chromosomes Cancer 4:257–263 (1992).
32.
Tian YB, He SY, Ge CR: Gayal [in Chinese]. J Yellow Cattle Sci 24:1–8 (1998).
33.
Walker EP, Warnick F, Hamlet SE, Lange KI, Davis MA, Uible HE, Wright RF: Mammals of the World, 2nd ed., Vol. I (The Johns Hopkins Press, Baltimore 1968).
34.
Wang W, Lan H: Rapid and parallel chromosomal number reductions in muntjac deer inferred from mitochondrial DNA phylogeny. Mol Biol Evol 17:1326–1333 (2000).
35.
Wang YX, Ma SL, Li CY: The taxonomy, distribution and status of forest musk deer in China, in Ohtaishi N, Sheng HL (eds): Deer of China: Biology and Management, pp 22–30 (Elsevier Science, Tokyo 1993).
36.
Yang F: Chromosome evolution of the muntjacs: Inferences from Molecular Cytogenetics. Ph.D. thesis, University of Cambridge (1998).
37.
Yang F, Carter NP, Shi L, Ferguson-Smith MA: A comparative study of karyotypes of muntjacs by chromosome painting. Chromosoma 103:642–652 (1995).
38.
Yang F, Müller S, Just R, Ferguson-Smith MA: Comparative chromosome painting in mammals: Human and the Indian muntjac (Muntiacus muntjak vaginalis). Genomics 39:396–401 (1997a).
39.
Yang F, O’Brien PCM, Wienberg J, Ferguson-Smith MA: A reappraisal of the tandem fusion theory of karyotype evolution in the Indian muntjac using chromosome painting. Chromosome Res 5:109–117 (1997b).
40.
Yang F, O’Brien PCM, Wienberg J, Neitzel H, Lin CC, Ferguson-Smith MA: Chromosomal evolution of the Chinese muntjac (Muntiacus reevesi). Chromosoma 106:37–43 (1997c).
41.
Yang F, O’Brien PCM, Milne BS, Graphodatsky AS, Solanky N, Trifonov V, Rens W, Sargan D, Ferguson-Smith MA: A complete comparative chromosome map for the dog, red fox, and human and its integration with canine genetic maps. Genomics 62:189–202 (1999).
42.
Yang F, Fu B, O’Brien PCM, Nie WH, Ryder OA, Ferguson-Smith MA: Refined genome-wide comparative map of the domestic horse, donkey and human based on cross-species chromosome painting: insight into the occasional fertility of mules. Chromosome Res 12:65–76 (2004).
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