In this study, we examine the morphology, mitotic stability, meiotic behavior and the composition of heterochromatin of B chromosomes in Cestrum intermedium and C. strigilatum. The results showed that B chromosome number shows intraindividual variation in the root meristem, which seems to lead to a slight rate of B elimination in this somatic tissue. B chromosomes in both species were similar in size and shape, but differed with regard to the type, size and distribution of heterochromatin. Possible evolutionary pathways for B chromosome origin in Cestrum are discussed.   

Berg C, Greilhuber J: Cold-sensitive chromosome regions and heterochromatin in Cestrum (Solanaceae): C. strigilatum, C. fasciculatum, and C. elegans. Plant Syst Evol 185:133–151 (1993a).
Berg C, Greilhuber J: Cold-sensitive regions and heterochromatin in Cestrum aurantiacum (Solanaceae). Plant Syst Evol 185:259–273 (1993b).
Beukeboom LW: Bewildering Bs: an impression of the 1st B-chromosome conference. Heredity 73:328–336 (1994).
Bold HC: Bristol’s solution and medium. (1949). Available at:
Camacho JPM, Sharbel TF, Beukeboom LW: B chromosome evolution. Phil Trans R Soc Lond B 355:163–178 (2000).
Cheng YM, Lin BY: Cloning and characterization of maize B chromosome sequences derived from microdissection. Genetics 164:299–310 (2003).
Cuadrado A, Jouve N: Mapping and organization of highly-repeated DNA sequences by means of simultaneous and sequential FISH and C-banding in 6x-Triticale. Chromosome Res 2:331–338 (1994a).
Cuadrado A, Jouve N: Highly repetitive sequences in B chromosomes of Secale cereale revealed by fluorescence in situ hybridization. Genome 37:709–712 (1994b).
Dhar MK, Friebe B, Koul AK, Gill BS: Origin of an apparent B chromosome by mutation, chromosome fragmentation and specific DNA sequence amplification. Chromosoma 111:332–340 (2002).
Franks TK, Houben A, Leach CR, Timmis JN: The molecular organisation of a B chromosome tandem repeat sequence from Brachycome dichromosomatica. Chromosoma 105:223–230 (1996).
Guerra M: Patterns of heterochromatin distribution in plant chromosomes. Gen Mol Biology 23:1029–1041 (2000).
Hossain MA, Bauchan GR: Identification of B chromosomes using Giemsa banding in Medicago. J Heredity 90:428–429 (1999).
Houben A, Leach CR, Verlin D, Rofe R, Timmis JN: A repetitive DNA sequence common to the different B chromosomes of the genus Brachycome. Chromosoma 106:513–519 (1997).
Houben A, Thompson N, Ahne R, Leach CR, Verlin D, Timmis JN: A monophyletic origin of the B chromosomes of Brachycome dichromosomatica (Asteraceae). Plant Syst Evol 219:127–135 (1999).
Puertas MJ: Nature and evolution of B chromosomes in plants: A non-coding but information-rich part of plant genomes. Cytogenet Genome Res 96:198–205 (2002).
Qi ZX, Zeng H, Li XL, Chen CB, Song WQ, Chen RY: The molecular characterization of maize B chromosome specific AFLPs. Cell Res 12:63–68 (2002).
Schwarzacher TP, Ambros P, Schweizer D: Application of Giemsa banding to orchid karyotype analysis. Plant Syst Evol 134:293–297 (1980).
Smith LB, Downs RJ: Solanáceas, in Reitz PR (ed): Flora Ilustrada Catarinense. Part 1, pp 1–321 (Biblioteca Superior de Cultura, Itajaí 1966).
Stark EA, Connerton I, Bennett ST, Barnes SR, Parker JS, Forster JW: Molecular analysis of the structure of the maize B-chromosome. Chromosome Res 4:15–23 (1996).
Sýkorová E, Yoong Lim K, Fajkus J, Leitch AR: The signature of the Cestrum genome suggests an evolutionary response to the loss of (TTTAGGG)n telomeres. Chromosoma 112:164–172 (2003).
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.