The use of improved cytogenetic techniques such as fluorescent in situ hybridization (FISH) has offered important methodologies for cytotaxonomic and evolutionary studies. In particular, the mapping of 5S rDNA sites has proved to be an excellent marker in the study of different organisms and, more recently, in fish. In the present work, the FISH technique was used to map the 5S rDNA sites in the chromosomes of eight neotropical fish species from the Paraíba do Sul river basin, four of these belonging to the order Characiformes, family Characidae, genus Astyanax (A. scabripinnis, A. parahybae, A. giton and A. intermedius) and four to the order Siluriformes, family Loricariidae (Neoplecostomus microps, Harttia loricariformis, Hypostomus affinis and Upsilodus sp.). Karyotype evolution aspects of the analyzed groups are discussed.   

Almeida-Toledo LF, Ozouf-Costaz C, Foresti F, Bonillo C, Porto-Foresti F, Daniel-Silva MFZ: Conservation of the 5S-bearing chromosome pair and co-localization with major rDNA clusters in five species of Astyanax (Pisces, Characidae). Cytogenet Genome Res 97:229–233 (2002).
Alves AL: Análise da evolução dos gêneros da subfamília hemipsilichthiinae (Ostariophysi, Siluriformes, Loricariidae) com base em caracteres cromossômicos e de DNA mitocondrial. MSc Thesis, Universidade Estadual Paulista/Sao Paulo, Brazil (2002).
Armbruster JW: Phylogenetic relationships of the suckermouth armored catfishes of the Rhinelepis group (Loricariidae: Hypostominae). Copeia 1998:620–636 (1997).
Artoni RF, Bertollo LAC: Trends in the karyotype evolution of Loricariidae fish (Siluriformes). Hereditas 134:201–210 (2001).
Bertollo LAC, Takahashi CS, Moreira-Filho O: Cytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae). Rev Bras Genet 1:103–120 (1978).
Bezerra DD: Estudos citogenéticos populacionais em Hoplerythrinus unitaeniatus (Pisces, Erythrinidae). Análise da biodiversidade. MSc Thesis. Universidade de São Carlos/Sao Paulo, Brazil (2002).
Born GG, Bertollo LAC: An XX/XY sex chromosome system in a fish species, Hoplias malabaricus, with a polymorphic NOR-bearing X chromosome. Chromosome Res 8:111–118 (2000).
de Jesus CM, Moreira-Filho O: Cytogenetic studies in some Apareiodon species (Pisces, Parodontidae). Cytologia 65:397–402 (2000).
Ferro DAM, Néo DM, Moreira-Filho O, Bertollo A: Nucleolar organizing regions, 18S and 5S rDNA in Astyanax scabripinnis (Pisces, Characidae): populations distribution and functional diversity. Genetica 110:55–62 (2001).
Kavalco KF: Contribuição citogenética à análise da biodiversidade da ictiofauna das nascentes do rio Paraitinga. MSc Thesis. Universidade de São Carlos/Sao Paulo, Brazil (2003).
LeGrande WH: Chromosomal evolution in North American catfishes (Siluriformes: Ictaluridae) with particular emphasis on the madtoms, Nocturus. Copeia 1981:33–52 (1981).
Martins C, Galleti Jr PM: Chromosomal localization of 5S rDNA genes in Leporinus fish (Anostomidae, Characiformes). Chromosome Res 7:363–367 (1999).
Martins C, Galetti Jr PM: Conservative distribution of 5S rDNa loci in Schizodon (Pisces, Anostomidae) chromosomes. Chromosome Res 8:353–355 (2000).
Martins C, Galetti Jr PM: Organization of 5S rDNA in species of the fish Leporinus: two different genomic locations are characterized by distinct nontranscribed spacers. Genome 44:903–910 (2001).
Melo FAG: Revisão taxonômica das espécies do gênero Astyanax Baird and Girard, 1854 (Teleostei: Characiformes: Characidae) da região da Serra dos αrgãos. Rio de Janeiro. Arq Mus Nac 59:1–46 (2001).
Moreira-Filho O, Bertollo LAC, Galetti Jr PM: Structure and variability of nucleolar organizing regions in Parodontidae fish. Can J Genet Cytol 26(5): 564–568 (1984).
Pinkel D, Straume T, Gray JW: Cytogenetic analysis using quantitative, high-sensitivity, fluorescence in situ hybridization. Proc Natl Acad Sci USA 83:2934–2938 (1986).
Schweizer D, Loidl J: A model for heterochromatin dispersion and the evolution of C band patterns. Chromosome Today 9:61–74 (1987).
Volff JN, Schartl M: Variability of genetic sex determination in poeciliid fishes. Genetica 111:101–110 (2001).
Volff JN, Körting C, Sweeney K, Schartl M: The non-LTR retrotransposon Rex3 from the fish Xiphophorus is widespread among teleosts. Mol Biol Evol 16:1427–1438 (1999).
Wasko AP, Martins C, Wright JM, Galetti Jr PM: Molecular organization of 5S rDNA in fishes of the genus Brycon. Genome 44:893–902 (2001).
Weitzman SH, Malabarba LR: Perspectives about the phylogeny and classification of the Characidae (Teleostei: Characiformes), in Malabarba LR, Reis RE, Vari RP, Lucena ZMS, Lucena CA (eds): Phylogeny and Classification of Neotropical Fishes, pp 161–170 (EDIPUCRS Porto Alegre-RS, 1998).
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.