Due to their high sequence diversity even among closely related species, satellite DNA sequences can be a useful molecular marker for phylogenetic and taxonomic analyses. To characterize the satellite DNA in the genome of a native muntjac species of Taiwan, the Formosan muntjac, satellite DNA clones representing three different cervid satellite DNA families from this species were isolated and analyzed. Genomic organization study of these satellite DNAs was also undertaken. Three Formosan muntjac satellite DNA clones were obtained and designated as FM-satI (1,391 bp), FM-satII (1,143 bp) and FM-satIV (1,103 bp), and found to share ∼82, 81 and 98% sequence homology with the Chinese muntjac satellite I clone (C5), Indian muntjac satellite II clone (Mmv-0.7) and Chinese muntjac satellite IV clone (MR-1.0), respectively. These three satellite DNA families are organized in a pter←FM-satII–FM-satIV–FM-satI→qter orientation in the centromeric region with satII closely associated with the telomeric sequences. Satellite DNA sequence comparison, in combination with chromosome data concludes that the Formosan muntjac is likely a subspecies of M. reevesi, closely related to the Chinese muntjac. With the kinetochore satellite II DNA co-localizing with the telomeric sequences, the Formosan muntjac chromosomes could be truly telocentric.   

1.
Blower MD, Sullivan BA, Karpen GH: Conserved organization of centromeric chromatin in flies and human. Dev Cell 2:319–330 (2002).
2.
Buntjer JB, Nijman IJ, Zijlstra C, Lenstra JA: A satellite DNA element specific for roe deer (Capreolus capreolus). Chromosoma 107:1–5 (1998).
3.
Charlesworth B, Sniegowski P, Stephan W: The evolutionary dynamics of repetitive DNA in eukaryotes. Nature 371:215–220 (1994).
4.
Dod B, Mottez E, Desmarais E, Bonhomme F, Roizes G: Concerted evolution of light satellite DNA in genus Mus implies amplification and homogenization of larger blocks of repeat. Mol Biol Evol 6:478–491 (1989).
5.
Elder JF, Turner BJ: Concerted evolution of repetitive DNA sequences in eukaryotes. Q Rev Biol 70:297–320 (1995).
6.
Garrido-Ramos MA, de la Herran R, Jamilena M, Lozano R, Rejon Rejon C, Rejon Rejon M: Evolution of centromeric satellite DNA and its use in phylogenetic study of the Sparidae family (Pisces, Perciformes). Mol Phylogenet Evol 12:200–204 (1999).
7.
Kato M: Evaluation of intra- and interspecific divergence of satellite DNA sequences by nucleotide frequency calculation and pairwise sequence comparison. Biol Proc Online 5:63–68 (2003).
8.
Lee C, Ritchie DBC, Lin CC: A tandemly repetitive, centromeric DNA sequence from the Canadian woodland caribou (Rangifer tarandus caribou): its conservation and evolution in several deer species. Chromosome Res 2:293–306 (1994).
9.
Lee C, Court DR, Cho C, Haslett J, Lin CC: High-order organization of subrepeats and the evolution of cervid satellite I DNA. J Mol Evol 44: 327–335 (1997).
10.
Lee C, Stanyon R, Lin CC, Ferguson-Smith MA: Conservation of human gamma-X centromeric DNA among primates with an autosomal location in certain Old world monkeys. Chromosome Res 7:43–47 (1999).
11.
Levinson G, Gutman GA: Slipped-strand mispairing: a major mechanism for sequence evolution. Mol Biol Evol 4:203–221 (1987).
12.
Li YC, Lee C, Sanoudou D, Hseu TH, Li SY, Lin CC: Interstitial colocalization of two cervid satellite DNAs involved in the genesis of the Indian muntjac karyotype. Chromosome Res 8:363–373 (2000a).
13.
Li YC, Lee C, Hseu TH, Li SY, Lin CC: Direct visualization of the genomic distribution and organization of two cervid centromeric satellite DNA families. Cytogenet Cell Genet 89:192–198 (2000b).
14.
Li YC, Lee C, Chang WS, Li SY, Lin CC: Isolation and identification of a novel satellite DNA family highly conserved in several Cervidae species. Chromosoma 111:176–183 (2002).
15.
Lin CC, Sasi R, Fan YS, Chen ZQ: New evidence for tandem chromosome fusions in the karyotypic evolution of Asian muntjacs. Chromosoma 101:19–24 (1991).
16.
Maniatis T, Fritsch EF, Sambrook J: Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1982).
17.
Modi WS: Comparative analyses of heterochromatin in Microtus: sequence heterogeneity and localized expansion and contraction of satellite DNA arrays. Cytogenet Cell Genet 62:142–148 (1993).
18.
Plucienniczak A, Skowronski J, Jaworski J: Nucleotide sequence of bovine 1.715 satellite DNA and its relation to other bovine satellite sequences. J Mol Biol 158:293–304 (1982).
19.
Qureshi SA, Blake RD: Sequence characteristics of a cervid DNA repeat family. J Mol Evol 40:400–404 (1995).
20.
Vafa O, Shelby RD, Sullivan KF: CENP-A associated complex satellite DNA in the kinetochore of the Indian muntjac. Chromosoma 108:367–374 (1999).
21.
Walsh JB: Persistence of tandem arrays: implications for satellite DNA and simple-sequence DNAs. Genetics 115:553–567 (1987).
22.
Warburton PE, Cooke CA, Bourassa S, Vafa O, Sullivan BA, Stetten G, Gimelli G, Warburton D, Tyler-Smith C, Sullivan KF, Poirier GG, Earnshaw WC: Immunolocalization of CENP-A suggests a distinct nucleosome structure at the inner kinetochore plate of active centromeres. Curr Biol 7:901–904 (1997).
23.
Wilson DE, Reeder DAM: Mammal species of the world: A taxonomic and geographic reference. Second edition (Smithsonian Institute Press, Washington, DC 1993).
24.
Yang F, Carter NP, Shi L, Ferguson-Smith MA: A comparative study of karyotypes of muntjacs by chromosome painting. Chromosoma 103:642–652 (1995).
25.
Yang F, O’Brien PCM, Wienberg J, Neitzel H, Lin CC, Ferguson-Smith MA: Chromosomal evolution of Chinese muntjacs (Muntiacus reevesi). Chromosoma 106:37–43 (1997).
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2004
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