We report the isolation and characterization of human contactin 4 (CNTN4), a brain-derived, immunoglobulin superfamily molecule-2 (alias BIG-2) as a candidate gene responsible for the differentiation potential of human neuroblastoma cells. Northern blot analysis showed highest CNTN4 expression in testes, thyroid, small intestine, uterus and brain. Induction of CNTN4 mRNA expression in human neuroblastoma tumor cells treated with retinoic acid correlated with a block in retinoid-induced neuritogenesis. Our findings suggest a role for human contactin 4 protein in the response of neuroblastoma cells to differentiating agents.   

1.
Balmer JE, Blomhoff R: Gene expression regulation by retinoic acid. J Lipid Res 43:1773–1808 (2002).
2.
Bauer D, Müller H, Reich J, Riedel H, Ahrenkiel V, Warthoe P, Strauss M: Identification of differentially expressed mRNA species by an improved display technique (DDRT-PCR). Nucl Acids Res 21:4272–4280 (1993).
3.
Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159 (1987).
4.
Drwinga HL, Toji LH, Kim CH, Greene AE, Mulivor RA: NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. Genomics 16:311–314 (1993).
5.
Kleinschmidt-DeMasters BK, Orr EA, Savelieva E, Owens GC, Kruse CA: Paucity of retinoic acid receptor alpha (RAR?) nuclear immunostaining in gliomas and inability of retinoic acid to influence neural cell adhesion molecule (NCAM) expression. J Neurooncol 41:31–42 (1999).
6.
Marshall GM, Cheung B, Stacey KP, Camacho ML, Simpson AM, Kwan E, Smith S, Haber M, Norris MD: Increased retinoic acid receptor gamma expression suppresses the malignant phenotype and alters the differentiation potential of human neuroblastoma cells. Oncogene 11:485–491 (1995).
7.
Matthay KK, Villablanca JG, Seeger RC, Stram DO, Harris RE, Ramsay NK, Swift P, Shimada H, Black CT, Brodeur GM, Gerbing RB, Reynolds CP: Treatment of high risk neuroblastoma with intensive chemotherapy, radiotherapy, autologous bone marrow transplantation, and 13-cis-retinoic acid. New Eng J Med 341:1165–1173 (1999).
8.
Phimister E, Kiely F, Kemshead JT, Patel K: Expression of neural cell adhesion molecule (NCAM) isoforms in neuroblastoma. J Clin Pathol 44:580–585 (1991).
9.
Sidell N, Altman A, Haussler MR, Seeger RC: Effects of retinoic acid (RA) on the growth and the phenotypic expression of several human neuroblastoma cell lines. Exp Cell Res 148:21–30 (1983).
10.
Yoshihara Y, Kawasaki M, Tani A, Tamada A, Nagata S, Kagamiyama H, Mori K: BIG-1: A new TAG-1/F3-related member of the immunoglubulin superfamily with neurite outgrowth-promoting activity. Neuron 13:415–426 (1994).
11.
Yoshihara Y, Kawasaki M, Tamada A, Nagata S, Kagamiyama H, Mori K: Overlapping and differential expression of BIG-2, BIG-1, TAG-1, and F3: Four members of an axonal-associated cell adhesion molecule subgroup of the immunoglobulin superfamily. J Neurobiol 28:51–69 (1995).
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2003
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