... (such as the thickness of the viable epidermis and the shape of the dermal-epidermal junction (DEJ)) and the makeup and composition of the cells forming that structure (such as how orderly the pattern of keratinocytes are in the viable epidermis). Analysis of these two classes of features has been attempted in a variety...

... of SCC with human papilloma virus (HPV) has been suggested but not confirmed. The detection of high levels of HPV DNA in the SCC of OTRs suggests that HPV may play a role in the formation of SCC, especially in these patients [ 15 , 16 . ]However, recent data have clearly shown that keratinocyte infection...

... are optical coherence tomography and high-frequency ultrasound. Both are capable of resolving some non-melanoma skin cancer tumors, but they lack the resolution to define cellular structure. Keratinocyte morphology appears to be one of the most robust features used to define actinic keratoses. Reflectance...

... and confocal microscopy. Much OCT research has been performed on keratinocyte dysplasia and neoplasia, primarily including basal cell carcinoma (BCC) and actinic keratosis. In regards to BCC and actinic keratosis, architectural disarray of the epidermis is an overall characteristic finding in OCT images. OCT...

... Abstract Neonatal and adult keratinocytes isolated from thin sections of splitthickness skin by trypsin-release show a preferential and strong attachment to collagen when compared to plastic, fibronectin-coated plastic, glass, or agar gels. We have investigated the reactive groups...

... Abstract Cultures of human keratinocytes were established according to the technique of Rheinwald and Green.1 When cultures were exposed to [14C] leucine, the uptake of leucine and increase in the specific activity of 6 urea-extractable polypeptides was prompt—each keratin achieved 50% of its...

... Abstract SummaryA method to detect and analyze the proteins transferred to a collagen substrate following interaction of normal human keratinocytes with a collagen substrate is described. Keratinocytes are allowed to attach to a collagen substrate for 1 hour, and the attached cells are lysed...

... Abstract SummaryThe specific binding of lectins to the cell surfaces in particular epidermal strata indicates that oligosaccharide moieties of the glycoconjugates on the cell membrane are altered as the keratinocyte differentiates. The disappearance of binding by the Bandeiraea simplicifolia...

... Abstract Human keratinocytes in culture were treated with dibutyryl cyclic AMP (DBcAMP) at concentrations of 10-3 and 10-4 M, and labeled with [3H]histidine for 48 hours. The proteins of the keratinocytes were serially extracted in 0.25 M sucrose, 0.1 M sodium citrate-citric acid (pH 2.6),6 M...

..., and certain kinds of amino acids. Large amounts of nuclear proteins appeared to be transformed from nuclear proteins into certain structural proteins in keratinocytes. ...

... are unique in formation of a multilayered cellular sheet in which the keratinocytes form a structure resembling the epidermis in vivo, and keratinize at the top. In the early days of the primary culture of human keratinocytes, when the cells proliferate to form a monolayer sheet, direct access to cell...