Cellular Diagnostics: Basic Principles, Methods and Clinical Applications of Flow Cytometry
This book is the updated English version of the 2006 German bestseller 'Zelluläre Diagnostik', a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.
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Table of Contents
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1 - 28: Cytometry – a Definitive History of the Early DaysByJ. Paul RobinsonJ. Paul RobinsonCytometry Laboratories, Bindley Bioscience Center, Purdue University, West Lafayette, IN, USASearch for other works by this author on:
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29 - 52: Concept Developments in Flow CytometryByGünter ValetGünter ValetMax Planck Institute for Biochemistry, Martinsried, GermanySearch for other works by this author on:
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53 - 88: Technical Background and Methodological Principles of Flow CytometryByGregor RotheGregor RotheLaborzentrum Bremen, LADR Group, Bremen, GermanySearch for other works by this author on:
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89 - 106: Technical and Methodological Basics of Slide-Based CytometryByAnja Mittag;Anja MittagaTranslational Center for Regenerative Medicine,Search for other works by this author on:Jozsef Bocsi;Jozsef BocsibDepartment of Pediatric Cardiology, Heart Center, University of Leipzig,Search for other works by this author on:Wiebke Laffers;Wiebke LafferscDepartment of Otorhinolaryngology/Head and Neck Surgery, University of Bonn, GermanySearch for other works by this author on:Andreas O.H. GerstnerAndreas O.H. GerstnercDepartment of Otorhinolaryngology/Head and Neck Surgery, University of Bonn, GermanySearch for other works by this author on:
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107 - 140: Selection and Combination of Fluorescent DyesByMatthias Schiemann;Matthias SchiemannInstitute for Medical Medicine, Immunology and Hygiene, Technical University Munich, GermanySearch for other works by this author on:Dirk Hans BuschDirk Hans BuschInstitute for Medical Medicine, Immunology and Hygiene, Technical University Munich, GermanySearch for other works by this author on:
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141 - 158: Fluorescence Resonance Energy Transfer (FRET)ByGyörgy Vámosi;György Vámosia Cell Biology and Signaling Research Group of the Hungarian Academy of Sciences,c Research Center for Molecular Medicine recognized as Center of Excellence by the European Commission Medical and Health Science Center, University of Debrecen, Hungary,Search for other works by this author on:György Vereb;György Verebb Department of Biophysics and Cell Biology,c Research Center for Molecular Medicine recognized as Center of Excellence by the European Commission Medical and Health Science Center, University of Debrecen, Hungary,Search for other works by this author on:Andrea Bodnár;Andrea Bodnára Cell Biology and Signaling Research Group of the Hungarian Academy of Sciences,c Research Center for Molecular Medicine recognized as Center of Excellence by the European Commission Medical and Health Science Center, University of Debrecen, Hungary,Search for other works by this author on:Katalin Tóth;Katalin Tóthc Research Center for Molecular Medicine recognized as Center of Excellence by the European Commission Medical and Health Science Center, University of Debrecen, Hungary,Search for other works by this author on:Nina Baudendistel;Nina Baudendistelc Research Center for Molecular Medicine recognized as Center of Excellence by the European Commission Medical and Health Science Center, University of Debrecen, Hungary,d Division Biophysics of Macromolecules, German Cancer Research Center, Heidelberg, GermanySearch for other works by this author on:Sándor DamjanovichSándor Damjanovicha Cell Biology and Signaling Research Group of the Hungarian Academy of Sciences,c Research Center for Molecular Medicine recognized as Center of Excellence by the European Commission Medical and Health Science Center, University of Debrecen, Hungary,Search for other works by this author on:
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159 - 177: Quality Control and StandardizationByAnja Mittag;Anja MittagaTranslational Center for Regenerative Medicine (TRM), University of Leipzig,Search for other works by this author on:Dominik Lenz;Dominik LenzbDepartment of Pediatric Cardiology, Heart Center Leipzig, GermanySearch for other works by this author on:Attila TarnokAttila TarnokbDepartment of Pediatric Cardiology, Heart Center Leipzig, GermanySearch for other works by this author on:
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178 - 189: Flow-Cytometric Cell SortingByMichael CrossMichael CrossDivision of Hematology/Oncology, IZKF, Leipzig, GermanySearch for other works by this author on:
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190 - 199: Characterization of T-LymphocytesByRichard Mauerer;Richard MauererSynlab Laboratory, Weiden, GermanySearch for other works by this author on:Rudolf GruberRudolf GruberSynlab Laboratory, Weiden, GermanySearch for other works by this author on:
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200 - 210: T-Cell Receptor Repertoire Analysis by Flow CytometryByDieter Kabelitz;Dieter KabelitzInstitute of Immunology, University Hospital Schleswig-Holstein Campus Kiel, Kiel, GermanySearch for other works by this author on:Daniela WeschDaniela WeschInstitute of Immunology, University Hospital Schleswig-Holstein Campus Kiel, Kiel, GermanySearch for other works by this author on:
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211 - 229: Characterization of B-LymphocytesByMichael Schlesier;Michael SchlesierDepartment of Rheumatology and Clinical Immunology, Medical Clinic, University Hospital of Freiburg, GermanySearch for other works by this author on:Klaus WarnatzKlaus WarnatzDepartment of Rheumatology and Clinical Immunology, Medical Clinic, University Hospital of Freiburg, GermanySearch for other works by this author on:
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230 - 260: Characterization of Natural Killer CellsByRoland Jacobs;Roland JacobsClinic for Immunology and Rheumatology Hanover, GermanySearch for other works by this author on:Reinhold E. SchmidtReinhold E. SchmidtClinic for Immunology and Rheumatology Hanover, GermanySearch for other works by this author on:
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261 - 271: Lymphocyte Subsets in the Peripheral Blood of Healthy ChildrenByUlrich Sack;Ulrich SackaInstitute of Clinical Immunology and Transfusion Medicine, Medical Faculty,Search for other works by this author on:Fee Gerling;Fee GerlingaInstitute of Clinical Immunology and Transfusion Medicine, Medical Faculty,Search for other works by this author on:Attila TárnokAttila TárnokbDepartment of Pediatric Cardiology, Cardiac Center Leipzig GmbH, University of Leipzig, Leipzig, GermanySearch for other works by this author on:
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272 - 285: Quantitative Assessment of Plasmacytoid and Myeloid Dendritic CellsByRüdiger V. SorgRüdiger V. SorgInstitute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University Hospital Düsseldorf, GermanySearch for other works by this author on:
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286 - 304: Peripheral Blood Stem CellsByStefan FruehaufStefan FruehaufParacelsus-Klinik, Osnabrück, GermanySearch for other works by this author on:
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305 - 316: Endothelial Progenitor CellsByJohannes C. FischerJohannes C. FischerInstitute for Transplantation Diagnostics and Cellular Therapeutics, Heinrich Heine University Düsseldorf, GermanySearch for other works by this author on:
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317 - 324: Mesenchymal Progenitor CellsByJohannes C. FischerJohannes C. FischerInstitute for Transplantation Diagnostics and Cellular Therapeutics, Heinrich Heine University Düsseldorf, GermanySearch for other works by this author on:
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325 - 342: Determination of Cell Physiological Parameters: pH, Ca2+, Glutathione, Transmembrane PotentialByAttila Tárnok;Attila TárnokaPediatric Cardiology, Cardiac Center Leipzig GmbH, University of Leipzig,Search for other works by this author on:Gregor RotheGregor RothebLaborzentrum Bremen, LADR Group, Bremen, GermanySearch for other works by this author on:
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343 - 376: Oxidative Burst, Phagocytosis and Expression of Adhesion MoleculesByAndreas Lun;Andreas LunaCentral Institute for Laboratory Medicine and Pathobiochemistry, Charité, Berlin,Search for other works by this author on:Ilka Schulze;Ilka SchulzebUniversity Hospital of Freiburg, Center for Pediatric and Adolescent Medicine, Freiburg i.Br.,Search for other works by this author on:Joachim Roesler;Joachim RoeslercClinic and Polyclinic for Pediatric Medicine, Carl Gustav Carus University Hospital, Dresden,Search for other works by this author on:Volker WahnVolker WahndCharité Center for Immunodeficiency, Berlin, GermanySearch for other works by this author on:
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377 - 389: Platelet Function and Thrombopoietic ActivityByAndreas Ruf;Andreas RufaCenter for Laboratory Medicine, Microbiology and Transfusion Medicine, Städtisches Klinikum Karlsruhe gGmbH, Karlsruhe,Search for other works by this author on:Stefan BarlageStefan BarlagebLaboratory Medicine, MVZ Leverkusen, Leverkusen, GermanySearch for other works by this author on:
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390 - 425: DNA and Proliferation Analysis by Flow CytometryByGero BrockhoffGero BrockhoffClinic for Gynecology and Obstetrics, Caritas Hospital St. Josef, University of Regensburg, GermanySearch for other works by this author on:
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426 - 458: Multidrug ResistanceByAlexandra Dorn-Beineke;Alexandra Dorn-BeinekeInstitute of Clinical Chemistry, Faculty for Clinical Medicine, University of Heidelberg, GermanySearch for other works by this author on:Cornelia KeupCornelia KeupInstitute of Clinical Chemistry, Faculty for Clinical Medicine, University of Heidelberg, GermanySearch for other works by this author on:
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459 - 475: Flow-Cytometric Analysis of Intracellularly Stained Cytokines, Phosphoproteins, and Microbial AntigensByIrina Lehmann;Irina LehmannaHelmholtz Center for Environmental Research – UFZ,Search for other works by this author on:Jörg LehmannJörg LehmannbFraunhofer Institute for Cell Therapy and Immunology, Leipzig, GermanySearch for other works by this author on:
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476 - 502: Detection of Antigen-Specific T-Cells using Major Histocompatibility Complex Multimers or Functional ParametersByAlexander Scheffold;Alexander ScheffoldaMiltonyi Biotex GmbH, Bergisch Gladbach,Search for other works by this author on:Dirk H. Busch;Dirk H. BuschbInstitute for Medical Microbiology, Immunology and Hygiene, Technical University Munich, Munich, Germany,Search for other works by this author on:Florian KernFlorian KerncBrighton and Sussex Medical School, University of Sussex, Brighton, UKSearch for other works by this author on:
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503 - 523: Immunophenotyping CD4 T-Cells to Monitor HIV DiseaseByFrancis F. MandyFrancis F. MandyInternational Centre for Infectious Diseases, Winnipeg, Manitoba, CanadaSearch for other works by this author on:
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524 - 539: Primary Immunodeficiency DiseasesByIlka Schulze;Ilka SchulzeaCenter for Pediatric and Adolescent Medicine, University Hospital Freiburg, Freiburg i. Br.,Search for other works by this author on:Volker WahnVolker WahnbCharité Immunodeficiency Center, Berlin, GermanySearch for other works by this author on:
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540 - 557: Diagnostics for Common Variable Immunodeficiency SyndromeByKlaus Warnatz;Klaus WarnatzDepartment of Rheumatology and Clinical Immunology, Medical Clinic, University Hospital Freiburg, Freiburg i. Br., GermanySearch for other works by this author on:Michael SchlesierMichael SchlesierDepartment of Rheumatology and Clinical Immunology, Medical Clinic, University Hospital Freiburg, Freiburg i. Br., GermanySearch for other works by this author on:
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558 - 579: Monitoring of Organ-Transplanted PatientsByAndreas Lun;Andreas LunaInstitute for Laboratory Medicine and Pathobiochemistry, Charité University Hospital, Berlin,Search for other works by this author on:Ulrich SackUlrich SackbInstitute for Clinical Immunology and Transfusion Medicine, Medical Faculty of the University of Leipzig, GermanySearch for other works by this author on:
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580 - 599: Flow-Cytometric Analysis of Cells from the Respiratory Tract: Bronchoalveolar Lavage Fluid and Induced SputumByDetlef LoppowDetlef LoppowLaboratory Dr. Kramer & Colleagues, LADR GmbH, Geesthacht, GermanySearch for other works by this author on:
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600 - 611: Pathophysiology and Immune Monitoring of SepsisByChristian Meisel;Christian MeiselInstitute for Medical Immunology, Charité University Hospital, Campus Mitte, Berlin, GermanySearch for other works by this author on:Hans-Dieter VolkHans-Dieter VolkInstitute for Medical Immunology, Charité University Hospital, Campus Mitte, Berlin, GermanySearch for other works by this author on:
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612 - 641: Flow-Cytometric Immunophenotyping of Acute LeukemiasByRichard Ratei;Richard RateiaHELIOS Klinikum Berlin-Buch, Charité University Hospital, Campus Buch, Berlin,Search for other works by this author on:Thomas Nebe;Thomas NebebUniversity of Heidelberg, Medical Faculty of Mannheim,Search for other works by this author on:Richard Schabath;Richard SchabathaHELIOS Klinikum Berlin-Buch, Charité University Hospital, Campus Buch, Berlin,Search for other works by this author on:Hans-Dieter Kleine;Hans-Dieter KleinecAnsomed AG, Rostock, GermanySearch for other works by this author on:Leonid Karawajew;Leonid KarawajewaHELIOS Klinikum Berlin-Buch, Charité University Hospital, Campus Buch, Berlin,Search for other works by this author on:Wolf-Dieter LudwigWolf-Dieter LudwigaHELIOS Klinikum Berlin-Buch, Charité University Hospital, Campus Buch, Berlin,Search for other works by this author on:
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642 - 667: Flow Cytometry in the Diagnosis of Non-Hodgkin’s LymphomasByMohammed WattadMohammed WattadKliniken Essen Süd, Evangelisches Krankenhaus Essen-Werden, GermanySearch for other works by this author on:
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668 - 679: Paroxysmal Nocturnal HemoglobinuriaByMartin GrünewaldMartin GrünewaldMedizinische Klinik I, Klinikum Heidenheim, GermanySearch for other works by this author on:
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680 - 686: Fetal ErythrocytesByPeter SedlmayrPeter SedlmayrInstitute of Cell Biology, Histology and Embryology, Center for Molecular Medicine, Medical University of Graz, AustriaSearch for other works by this author on:
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687 - 692: Flow-Cytometric Cross-Match for the Determination of Platelet AntibodiesByRainer Lynen;Rainer LynenDepartment of Transfusion Medicine, University Medical Center, Georg August University, Göttingen, GermanySearch for other works by this author on:Tobias J. LeglerTobias J. LeglerDepartment of Transfusion Medicine, University Medical Center, Georg August University, Göttingen, GermanySearch for other works by this author on:
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693 - 713: Residual Cells in Blood ProductsByMichael WötzelMichael WötzelLaboratory Dr. Reising-Ackermann and Partners, Leipzig, GermanySearch for other works by this author on:
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714 - 723: Enrichment of Disseminated Tumor CellsByJozsef Bocsi;Jozsef BocsiaPediatric Cardiology, Heart Center, University of Leipzig, Germany,Search for other works by this author on:Bela Molnar;Bela MolnarbInternal Medicine, Semmelweis University, Budapest, Hungary,Search for other works by this author on:Anja Mittag;Anja MittagcTranslational Center for Regenerative Medicine, University of Leipzig, GermanySearch for other works by this author on:Dominik LenzDominik LenzaPediatric Cardiology, Heart Center, University of Leipzig, Germany,Search for other works by this author on: