The neuropeptide arginine vasotocin (AVT) is well known to modulate both aggression and affiliation, yet few studies relate individual behavioral state to a quantitative assessment of AVT distribution in the brain. Here, using a wild population of beaugregory damselfish, Stegastes leucostictus, we assess: (1) the effect of AVT on courtship, and (2) with reference to our previous study on AVT modulation of aggression in this species, the relationship between AVT-like immunoreactive (ir) fiber distribution in the forebrain’s preoptic area and individual courtship and aggression levels. Exogenous AVT did not affect courtship, yet Manning compound, an arginine vasopressin (AVP) V1a receptor antagonist, significantly lowered but did not eradicate courtship. Consistent with AVT’s known facilitation of aggression in this species, the density of AVT-ir fibers in the preoptic area was significantly negatively correlated to aggression. Our findings match similar behavioral and immunoreactive patterns of neuropeptide secretion in other taxa. Unlike aggression, preoptic AVT-ir fiber density was not significantly correlated to individual courtship levels. The results suggest a differential involvement of preoptic AVT neurons and/or their receptors in supporting the expression of aggression and courtship.

Keywords:
Aggression, Arginine vasopressin, Arginine vasotocin, Courtship, Damselfish, Isotocin, Preoptic area
1.
Bamshad M, Novak MA, DeVries GJ (1994): Cohabitation alters vasopressin innervations and paternal behavior in prairie voles (Microtus ochrogaster). Physiol Behav 56:751–758.
2.
Bamshad M, Novak MA, DeVries GJ (1993): Sex and species-differences in the vasopressin innervations of sexually naïve and parental prairie voles, Microtus ochrogaster and meadow voles, Microtus pennsylvanicus. J Neuroendocrinol 5:247–255.
3.
Barberis C, Morin D, Durroux T, Mouillac B, Guillon G, Seyer R, Hibert M, Tribollet E, Manning M (1999): Molecular pharmacology of AVP and OT receptors and therapeutic potential. Drug News Persp 12:279–292.
4.
Bass AH, Forlano PM (2008): Neuroendocrine mechanisms of alternative reproductive tactics: The chemical language of social plasticity; in Oliveira RF, Taborsky M, Brockmann J (eds): Alternative Reproductive Tactics: An Integrative Approach. Cambridge University Press, pp 109–131.
5.
Bernard DJ, Abuav-Nussbaum R, Horton TH, Turek FW (1999): Photoperiodic effects on gonadotropin-releasing hormone (GnRH) content and the GnRH-immunoreactive neuronal system of male Siberian hamsters. Biol Reprod 60:272–276.
6.
Bester-Meredith JK, Marler CA (2001): Vasopressin and aggression in cross-fostered California mice (Peromyscus californicus) and white-footed mice (Permyscus leucopus). Horm Behav 40:51–64.
7.
Bester-Meredith JK, Young LJ, Marler CA (1999): Species differences in paternal behavior and aggression in Peromyscus and their association with vasopressin immunoreactivity and receptors. Horm Behav 36:25–38.
8.
Carneiro LA, Oliveira RF, Canário AVM, Grober MS (2003): The effect of arginine vasotocin on courtship behaviour in a blenniid fish with alternative reproductive tactics. Fish Physiol Biochem 28:241–243.
9.
Cleveland AL, Ludlow AM, Itzkowitz M, Draud M, Haley M (2003): Dominance relationships between male territorial neighbors in the beaugregory damselfish (Stegastes leucostictus). Behaviour 140:1021–1037.
10.
Cooper MA, Karom M, Huhman KL, Albers HE (2005): Repeated agonistic encounters in hamsters modulate AVP V1a receptor binding. Horm Behav 48:545–551.
11.
Chu J, Marler CA, Wilczynski W (1998): The effects of arginine vasotocin on the calling behavior of male cricket frogs in changing social contexts. Horm Behav 34:248–261.
12.
Delville Y, De Vries GJ, Ferris CF (2000): Neural connections of the anterior hypothalamus and agonistic behavior in golden hamsters. Brain Behav Evol 55:53–76.
13.
Dewan AK, Maruska KP, Tricas TC (2008): Arginine vasotocin neuronal phenotypes among congeneric territorial and shoaling reef butterflyfishes: species, sex and reproductive season comparisons. J Neuroendocrinol 20:1382–1394.
14.
Everts HGJ, De Ruiter AJH, Koolhaas JM (1997): Differential lateral septal vasopressin in wild-type rats: correlation with aggression. Horm Behav 31:136–144.
15.
Ferris CF, Melloni RH Jr, Koppel G, Perry KW, Fuller RW, Delville Y (1997): Vasopressin/serotonin interactions in the anterior hypothalamus control aggressive behavior in golden hamsters. J Neurosci 17:4331–4340.
16.
Forlano PM, Deitcher DL, Bass AH (2005): Distribution of estrogen receptor alpha mRNA in the brain and inner ear of a vocal fish with comparisons to sites of aromatase expression. J Comp Neurol 483:91–113.
17.
Forlano PM, Deitcher DL, Marchaterre MA, Bass AH (2010): Distribution of androgen receptor mRNA expression in vocal, auditory and neuroendocrine circuits in a teleost fish. J Comp Neurol 518:493–512.
18.
Gobrogge KL, Liu Y, Jia X, Wang Z (2007): Anterior hypothalamic neural activation and neurochemical associations with aggression in pair-bonded male prairie voles. J Comp Neurol 502:1109–1122.
19.
Godwin J, Sawby R, Warner RR, Crews D, Grober MS (2000): Hypothalamic arginine vasotocin mRNA abundance variation across sexes and with sex change in a coral reef fish. Brain Behav Evol 55:77–84.
20.
Goodson JL (2008): Nonapeptides and the evolutionary patterning of sociality. Progr Brain Res 170:3–15.
21.
Goodson JL (1998a): Territorial aggression and dawn song are modulated by septal vasotocin and vasoactive intestinal polypeptide in male field sparrows (Spizella pusilla). Horm Behav 34:67–77.
22.
Goodson JL (1998b): Vasotocin and vasoactive intestinal polypeptide modulate aggression in a territorial songbird, the violet eared waxbill (Estrildidae: Uraeginthus granatina). Gen Comp Endocrinol 111:233–244.
23.
Goodson JL, Adkins-Regan E (1999): Effect of vasotocin and vasoactive intestinal polypeptide infusions on courtship song and aggression in the male zebra finch (Taeniopygia guttata). J Neuroendocrinol 11:19–25.
24.
Goodson JL, Bass AH (2001): Social behavior functions and related anatomical characteristics of vasotocin/vasopressin systems in vertebrates. Brain Res Rev 35:246–265.
25.
Goodson JL, Bass AH (2000a): Vasotocin innervation and modulation of vocal-acoustic circuitry in the teleost Porichthys notatus. J Comp Neurol 422:363–379.
26.
Goodson JL, Bass AH (2000b): Forebrain peptides modulate sexually polymorphic vocal circuitry. Nature 403:769–772.
27.
Goodson JL, Evans AK, Bass AH (2003): Isotocin distributions in vocal fish: relationship to vasotocin and vocal-acoustic circuitry. J Comp Neurol 462:1–14.
28.
Goodson JL, Evans AK, Lindberg L, Allen CD (2005): Neuro-evolutionary patterning of sociality. Proc R Soc B 272:227–235.
29.
Goodson JL, Rinaldi J, Kelly AM (2009): Vasotocin neurons in the bed nucleus of the stria terminalis preferentially process social information and exhibit properties that dichotomize courtship and non-courting phenotypes. Horm Behav 55:197–202.
30.
Goodson JL, Wang Y (2006): Valence-sensitive neurons exhibit divergent functional profiles in gregarious and asocial species. Proc Natl Acad Sci (USA) 103:17013–17017.
31.
Greenwood AK, Wark AR, Fernald RD, Hofmann HA (2008): Expression of arginine vasotocin in distinct preoptic regions is associated with dominant and subordinate behavior in an African cichlid fish. Proc R Soc 275:2393–2402.
32.
Grober MS, George AA, Watkins KK, Carneiro LA, Oliveira RF (2002): Forebrain AVT and courtship in a fish with male alternative reproductive tactics. Brain Res Bull 57:423–425.
33.
Harding CH, Rowe SA (2003): Vasotocin treatment inhibits courtship in male zebra finches; concomitant androgen treatment inhibits this effect. Horm Behav 44:413–418.
34.
Hattori T, Wilczynski W (2009): Comparison of arginine vasotocin immunoreactivity differences in dominant and subordinate green anole lizards. Physiol Behav 96:104–107.
35.
Itzkowitz M (1991): Habitat selection and subsequent reproductive success in the beaugregory damselfish. Environ Biol Fish 30:287–293.
36.
Itzkowitz M (1985): Aspects of the population dynamics and reproductive success in the permanently territorial beaugregory damselfish. Mar Behav Physiol 12:57–69.
37.
Itzkowitz M, Haley M (1999): Are males with more attractive resources more selective in their mate preferences? A test in a polygynous species. Behav Ecol 10:366–371.
38.
Itzkowitz M, Itzkowitz DE, Shelly D (1995): Territory use and disuse in the beaugregory damselfish. Bull Mar Sci 57:653–662.
39.
Iwata T, Toyoda F, Yamamoto K, Kikuyama S (2000): Hormonal control of urodele reproductive behavior. Comp Biochem Physiol B 126:221–229.
40.
Koolhaas JM, Moor E, Hiemstra Y, Bohus B (1991): The testosterone-dependant vasopressinergic neurons in the medial amygdala and lateral septum: involvement in social behaviour in male rats; in Jard S, Jamison R (eds): Vasopressin. Paris/London, INSERM/Libbey, pp 213–219.
41.
Lema SC (2006): Population divergence in plasticity of the AVT system and its association with aggressive behaviors in a Death Valley pupfish. Horm Behav 50:183–193.
42.
Lema SC, Nevitt GA (2004a): Variation in vasotocin immunoreactiviy in the brain of recently isolated populations of a death valley pupfish, Cyprinodon nevadenis. Horm Behav 46:628–637.
43.
Lema SC, Nevitt GA (2004b): Exogenous vasotocin alters aggression during agonistic exchanges in male Amargosa River pupfish (Cyprinodon nevadenis amargosae). Gen Comp Endocrinol 135:300–309.
44.
Marler CA, Boyd SK, Wilczynski W (1999): Forebrain arginine vasotocin correlates of alternative mating strategies in cricket frogs. Horm Behav 36:53–61.
45.
Marler CA, Chu J, Wilczynski W (1995): Arginine vasotocin injection increases probability of calling in cricket frogs, but causes call change characteristic of less aggressive males. Horm Behav 29:554–570.
46.
Marsh KE, Creutz LM, Hawkins MB, Godwin J (2006): Aromatase immunoreactivity in the bluehead wrasse brain, Thalassoma bifasciatum: immunolocalization and co-regionalization with arginine vasotocin and tyrosine hydroxylase. Brain Res 1126:91–101.
47.
Maruska KP (2009): Sex and temporal variations of the vasotocin neuronal system in the damselfish brain. Gen Comp Endocrinol 160:194–204.
48.
Miranda JA, Oliveira RF, Carneiro LA, Santos RS, Grober MS (2003): Neurochemical correlates of male polymorphism and alternative reproductive tactics in the Azorean rock-pool blenny, Parablennius parvicornis. Gen Comp Endocrinol 132:183–189.
49.
Northcutt RG (2006): Connections of the lateral and medial divisions of the goldfish telencephalic pallium. J Comp Neurol 494:903–943.
50.
Northcutt RG (2008): Forebrain evolution in bony fishes. Brain Res Bull 75:191–205.
51.
Ronchi E, Aoki C, Krey LC, Pfaff DW (1992): Immunocytochemical study of GnRH and GnRH-associated peptide in male Syrian hamsters as a function of photoperiod and gonadal alterations. Neuroendocrinol 55:134–145.
52.
Rose JD, Moore FL (2002): Behavioral neuroendocrinology of vasotocin and vasopressin and the sensorimotor processing hypothesis. Front Neuroendocrinol 23:317–341.
53.
Rose JD, Kinnard JR, Moore FL (1995): Neurophysiological effects of vasotocin and corticosterone on medullary neurons: implications for hormonal control of amphibian courtship behavior. Neuroendocrinol 62:406–417.
54.
Saldahna CJ, Tuerk MJ, Yong-Hwan K, Fernandes AO, Arnold AP, Schlinger BA (2000): Distribution and regulation of telencephalic aromatase expression in the zebra finch revealed with a specific antibody. J Comp Neurol 423:619–630.
55.
Salek SJ, Sullivan CV, Godwin J (2001): Arginine vasotocin effects on courtship behavior in male white perch (Morone americana). Behav Brain Res 133:177–183.
56.
Santangelo N, Bass AH (2006): New Insights into neuropeptide modulation of aggression: field studies of arginine vasotocin in a territorial tropical damselfish. Proc R Soc B 273:3085–3092.
57.
Santangelo N, Itzkowitz M, Richter M, Haley MP (2002): Resource attractiveness of the male beaugregory damselfish and his decision to court or defend. Behav Ecol 13:676–681.
58.
Sapolsky RM, Romero LM, Munck AU (2000): How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions. Endocr Rev 21:55–89.
59.
Semsar K, Godwin J (2003): Social influences on the arginine vasotocin system are independent of gonads in a sex-changing fish. J Neurosci 23:4386–4393.
60.
Semsar K, Kandel FLM, Godwin J (2001): Manipulations of the AVT system shift social status and related courtship and aggressive behavior in the bluehead wrasse. Horm Behav 40:21–31.
61.
Semsar K, Klomberg KF, Marler C (1998): Arginine vasotocin increases calling-site acquisition by nonresident male grey treefrogs. Anim Behav 56:983–987.
62.
Snekser JL, Leese J, Ganim A, Itzkowitz M (2009): Caribbean damselfish with varying territory quality: correlated behaviors but not a syndrome. Behav Ecol 20:124–130.
63.
Tito MB, Hoover MA, Mingo AM, Boyd SK (1999): Vasotocin maintains multiple call types in the gray tree frog, Hyla versicolor. Horm Behav 36:166–175.
64.
Thompson RR, Walton JC (2009): Vasotocin immunoreactivity in goldfish brains: characterizing primitive circuits associated with social regulation. Brain Behav Evol 73:153–164.
65.
Trainor BC, Rouse KL, Marler CA (2002): Arginine vasotocin interacts with the social environment to regulate advertisement calling in the gray tree frog (Hyla veriscolor). Brain Behav Evol 61:165–171.
66.
Urbanski HF, Doan A, Pierce M (1991): Immunocytochemical investigation of luteinizing hormone-releasing hormone neurons in Syrian hamsters maintained under long or short days. Biol Reprod 44:687–692.
67.
Wang Z (1995): Species differences in the vasopressin-immunoreactive pathways in the bed nucleaus of the stria-terminalis and the medial amygdaloid nucleus in prairie voles (Microtus ochrogaster) and meadow voles (Microtus pennsylvanicus). Behav Neurosci 109:305–311.
68.
Wang ZX, Ferris CF, DeVries GJ (1994): Role of septal vasopressin innervations in parental behavior in prairie voles (Microtus ochrogaster). Proc Natl Acad Sci (USA) 91:400–404.
69.
Winslow JT, Hastings N, Carter SC, Harbaugh CR, Insel TR (1993): A role for central vasopressin in pair bonding in monogamous prairie voles. Nature 365:545–548.
70.
Wilczynski W, Lynch KS, O’Bryant E (2005): Current research in amphibians: studies integrating endocrinology, behavior, and neurobiology. Horm Behav 48:440–450.
71.
Young LJ, Winslow JT, Nilsen R, Insel TR (1997): Species differences in V1a receptor gene expression in monogamous and non-monogamous voles: behavioral consequences. Behav Neurosci 111:599–605.
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2010
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