In anurans, much is known about the role of the auditory midbrain in processing conspecific calls, but comparatively little is known about the role of the pallium. To address this deficiency, we investigated the induction of the immediate early gene egr-1 by natural mate chorus in the medial, dorsal, lateral, and ventral pallium of female túngara frogs. We found strong acoustically evoked egr-1 expression in the dorsal medial pallium (p < 0.01) and ventral pallium (p = 0.02), with a weaker effect in the lateral pallium (p = 0.05). In the ventral pallium, acoustically induced egr-1 expression was stronger in the anterior portion. Measures of movement and olfactory activity could not explain a significant portion of acoustically evoked pallial egr-1 expression. In contrast, egr-1 expression in the auditory midbrain covaried with egr-1 expression in the dorsal medial pallium and ventral pallium, suggesting that their activity was coupled with auditory activity. Taken together, these results suggest that the acoustically evoked egr-1 expression in the dorsal medial pallium and ventral pallium were a direct result of auditory stimulation. Furthermore, although both anatomical and electrophysiological evidence demonstrate that multiple modalities overlap in the frog pallium, our results show that a multimodal stimulus is not required to activate pallial neurons. Although the functional role of the frog pallium is not known, our results demonstrate that species-specific sounds activate spatially segregated and anatomically distinct areas of the frog pallium, inviting further investigation into the role of the frog pallium in acoustic communication.

Andersson MB (1994) Sexual Selection. Princeton: Princeton University Press.
Arak A (1988) Female mate selection in the natterjack toad: active choice or passive attraction? Behav Ecol Sociobiol 22:317–327.
Bachy I, Berthon J, Retaux S (2002) Defining pallial and subpallial divisions in the developing Xenopus forebrain. Mech Dev 117:163–172.
Bizley J, Nodal F, Bako V, Nelken I, King A (2007) Physiological and anatomical evidence for multisensory interactions in auditory cortex. Cereb Cortex 177:2172–2189.
Brox A, Ferreiro B, Puelles L, Medina L (2002) The telencephalon of the frog Xenopus based on calretinin immunostaining and gene expression patterns. Brain Res Bull 57:381–384.
Brox A, Puelles L, Ferreiro B, Medina L (2003) Expression of the genes GAD67 and Distal-less-4 in the forebrain of Xenopus laevis confirms a common pattern in tetrapods. J Comp Neurol 461:370–393.
Brox A, Puelles L, Ferreiro B, Medina L (2004) Expression of the genes Emx1, Tbr1, and Eomes (Tbr2) in the telencephalon of Xenopus laevis confirms the existence of a ventral pallial division in all tetrapods. J Comp Neurol 474:562–577.
Burmeister S, Mangiamele L, Lebonville C (2008) Acoustic modulation of immediate-early gene expression in the auditory midbrain of female túngara frogs. Brain Res 1190:105–114.
Butler A, Hodos W (1996) Comparative Vertebrate Neuroanatomy: Evolution and Adaptation. New York: Wiley-Liss.
Clayton D (2000) The genomic action potential. Neurobiol Learn Mem 74:185–216.
Edwards C, Alder T, Rose G (2002) Auditory midbrain neurons that count. Nat Neurosci 5:934–936.
Endepols H, Feng A, Gerhardt H, Schul J, Walkowiak W (2003) Roles of the auditory midbrain and thalamus in selective phonotaxis in female gray treefrogs (Hyla versicolor). Behav Brain Res 145:63–77.
Endepols H, Walkowiak W (2001) Integration of ascending and descending inputs in the auditory midbrain of anurans. J Comp Physiol A 186:1119–1133.
Ewert J, Dinges A, Finkenstadt T (1994) Species-universal stimulus responses, modified through conditioning, reappear after telencephalic lesions in toads. Naturwissenschaften 81:146–151.
Finkenstadt T, Ewert J (1988) Effects of visual associative conditioning on behavior and cerebral metabolic activity in toads. Naturwissenschaften 75:95–97.
Fuzessery Z, Feng A (1982) Frequency selectivity in the anuran auditory midbrain: single-unit responses to single and multiple tone stimulation. J Comp Physiol A 146:471–484.
Fuzessery Z, Feng A (1983) Mating call selectivity in the thalamus and midbrain of the leopard frog (Rana pipiens): Single and multiunit analyses. J Comp Physiol A 150:333–344.
Gerhardt H, Huber F (2002) Acoustic Communication in Insects and Anurans. Chicago: University of Chicago Press.
Gooler D, Feng A (1992) Temporal coding in the frog auditory midbrain: the influence of duration and rise-fall time on the processing of complex amplitude-modulated stimuli. J Neurophysiol 67:1–22.
Hall J, Feng A (1986) Neural analysis of temporally patterned sounds in the frog’s thalamus: Processing of pulse duration and pulse repetition rate. Neurosci Lett 63:215–220.
Hoke K, Burmeister S, Fernald R, Rand A, Ryan M, Wilczynski W (2004) Functional mapping of the auditory midbrain during mate call reception. J Neurosci 24:11264–11272.
Hoke K, Ryan M, Wilczynski W (2007) Integration of sensory and motor processing underlying social behaviour in tungara frogs. Proc R Soc Lond B 274:641–649.
Jarvis E (2004) Brains and Birdsong. In: Nature’s Music: The Science of Birdsong (Marler P, Slabberkoorn H, eds), pp. 226–271. New York: Elsevier-Academic Press.
Jarvis E, Nottebohm F (1997) Motor-driven gene expression. Proc Nat Acad Sci USA 97:4097–4102.
Jarvis E, Scharff C, Grossman M, Ramos J, Nottebohm F (1998) For whom the bird sings: context-dependent gene expression. Neuron 21:775–788.
Kaczmarek L, Chaudhuri A (1997) Sensory regulation of immediate-early gene expression in mammalian visual cortex: Implications for functional mapping and neural plasticity. Brain Res Rev 23:237–256.
Kicliter E (1979) Some telencephalic connections in the frog, Rana pipiens. J Comp Neurol 185:75–86.
Kicliter E, Northcutt R (1975) Ascending afferents to the telencephalon of Ranid frogs: An anterograde degeneration study. J Comp Neurol 161:239–254.
Laberge F, Mühlenbrock-Lenter S, Dicke U, Roth G (2008) Thalamo-telencephalic pathways in the fire-bellied toad Bombina orientalis. J Comp Neurol 508:806–823.
Laberge F, Roth G (2007) Organization of the sensory input to the telencephalon in the fire-bellied toad, Bombina orientalis. J Comp Neurol 502:55–74.
Marin O, Smeets W, Gonzalez A (1998) Basal ganglia organization in amphibians: Chemoarchitecture. J Comp Neurol 392:285–312.
Mello C, Vicario D, Clayton D (1992) Song presentation induces gene expression in the songbird forebrain. Proc Nat Acad Sci USA 89:6818–6822.
Molnar Z, Butler A (2002) Neuronal changes during forebrain evolution in amniotes: An evolutionary developmental perspective. Prog Brain Res 136:21–38.
Moreno N, Gonzalez A (2004) Localization and connectivity of the lateral amygdala in anuran amphibians. J Comp Neurol 479:130–148.
Mudry K, Capranica R (1980) Evoked auditory activity within the telencephalon of the bullfrog (Rana catesbeiana). Brain Res 182:303–311.
Neary T (1984) Anterior thalamic nucleus projections to the dorsal pallium in ranid frogs. Neurosci Lett 51:213–218.
Neary T (1990) The pallium of anuran amphibians. In: Comparative Structure and Evolution of Cerebral Cortex (Jones E, Peters A, eds), vol 8A, pp 107–138. New York: Plenum Press.
Neary T (1995) Afferent projections to the hypothalamus in ranid frogs. Brain Behav Evol 46:1–13.
Northcutt R, Ronan M (1992) Afferent and efferent connections of the bullfrog medial pallium. Brain Behav Evol 40:1–16.
Northcutt R, Royce G (1975) Olfactory bulb projections in the bullfrog Rana catesbeiana. J Morphol 145:251–268.
Poremba A, Saunders R, Crane A, Cook M, Sokoloff L, Mishkin M (2003) Functional mapping of the primate auditory system. Science 299:568–572.
Robertson J (1986) Female mate choice, male strategies and the role of vocalizations in the Australia frog Uperoleia rusgosa. Anim Behav 34:773–784.
Roth G, Grunwald W, Dicke U (2003) Morphology, axonal projection pattern, and responses to optic nerve stimulation of thalamic neurons in the fire-bellied toad Bombina orientalis. J Comp Neurol 461:91–110.
Roth G, Laberge F, Mühlenbrock-Lenter S, Grunwald W (2007) Organization of the pallium in the fire-bellied toad Bombina orientalis. I: Morphology and axonal projection pattern of neurons revealed by intracellular biocytin labeling. J Comp Neurol 501:443–464.
Roth G, Mühlenbrock-Lenter S, Grunwald W, Laberge F (2004) Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: An anterograde, retrograde and intracellular biocytin labeling study. J Comp Neurol 478:35–61.
Ryan M (1985) The Tungara Frog: A Study in Sexual Selection and Communication. Chicago: University of Chicago Press.
Scalia F, Halpern M, Knapp H, Riss W (1968) The efferent connexions of the olfactory bulb in the frog: a study of degenerating unmyelinated fibres. J Anat 103:245–262.
Stripling R, Volman S, Clayton D (1997) Response modulation in the zebra finch neostriatum: Relationship to nuclear gene regulation. J Neurosci 17:3883–3893.
Veenman C, Crzan D, Kern H, Rickmann M, Wahle P, van Mier P (1989) The anatomical substrate for telencephalic function. Adv Anat Embryol Cell Biol 117:1–110.
Walkowiak W, Berlinger M, Schul J, Gerhardt H (1999) Significance of forebrain structures in acoustically guided behavior in anurans. Eur J Morphol 37:177–181.
Westhoff G, Roth G (2002) Morphology and projection pattern of medial and dorsal pallial neurons in the frog Discoglossus pictus and the salamander Plethodon jordani. J Comp Neurol 445:97–121.
Wilczynski W, Endepols H (2007) Central auditory pathways in anuran amphibians: The anatomical basis of hearing and sound communication. In: Hearing and Sound Communication in Amphibians (Popper A, Feng A, Narins P, eds), vol 28. Berlin, Germany: Springer.
Worley P, Christy B, Nakabeppu Y, Bhat R, Cole A (1991) Constitutive expression of zif268 in neocortex is regulated by synaptic activity. Proc Nat Acad Sci USA 88:5106–5110.
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