In some pathological conditions excessive amounts of endolymph can accumulate and cause swelling, hydrops, of the membranous labyrinth. Reissner’s membrane in the cochlea will distend and may even rupture. We have studied the effects of acute hydrops, followed for up to 5–6 h, in a preparation that allows continuous monitoring of structural alterations in individual cells in Reissner’s membrane. This is accomplished by using laser confocal microscopy on the membrane visualized by labeling its cells with fluorescent dyes. In specimens subjected to hydrops it was observed that discrete structural defects developed in Reissner’s membrane. These were seen as lesions in single cells or in groups of cells in the epithelial layer. It is suggested that through these micro-lesions the electro-chemical environment of the organ of Corti can be altered causing hearing loss and tinnitus during hydrops.

Keywords:
Hydrops, Reissner’s membrane, Ménière’s disease, Hearing, Hearing loss, Tinnitus
1.
Albers FWJ, Groot JCMJ, Veldman JE, Huizing EH: Ultrastructure of the stria vascularis and Reissner’s membrane in experimental hydrops. Acta Otolaryngol 1987;104:202–210.
2.
Arima T, Uemura T, Yamamoto T: Structural features of the basal lamina in Reissner’s membrane of the guinea pig. Acta Otolaryngol 1985;100:194–200.
3.
Atkinson M: Ménière’s original papers. Acta Otolaryngol 1961( suppl 162):1–178.
4.
Counter SA, Bjelke B, Klason T, Chen Z, Borg E: Magnetic resonance imaging of the cochlea, spiral ganglia and eighth nerve of the guinea pig. Neuroreport 1999;10:473–479.
5.
Duvall AJ, Rhodes VT: Reissner’s membrane. An ultrastructural study. Arch Otolaryngol 1967;86:143–151.
6.
Felix H, Fraissinette A, Johnsson LG, Gleeson M: Morphological features of human Reissner’s membrane. Acta Otolaryngol 1993;113:321–325.
7.
Flock Å, Flock B: Hydrops in the cochlea can be induced by sound as well as by static pressure. Hearing Res 2000;150:175–188.
8.
Flock Å, Flock B, Scarfone E: Laser scanning confocal microscopy of the hearing organ: Fluorochrome-dependent cellular damage is seen after over-exposure. J Neurocytol 1998a;27:507–516.
9.
Flock Å, Scarfone E, Ulfendah M: Vital staining of the hearing organ: Visualization of cellular structure with confocal microscopy. Neuroscience 1998b;83:215–228.
10.
Fridberger A, van Maarseven J, Flock B, Ulfendahl M, Scarfone E, Flock Å: Pressure induced basilar membrane position shifts and the stimulus-evoked potentials in the low-frequency region of the guinea pig cochlea. Acta Physiol Scand 1997;161:239–252.
11.
Goodenough DA, Goliger JA, Paul DL: Connexins, connexons and intercellular communication. Annu Rev Biochem 1996;65:475–502.
12.
Hallpike CS, Cairns H: Observations on the pathology of Ménière’s syndrome. J Laryngol 1938;53:625–655.
13.
Hamill OP, Martinac B: Molecular basis of mechanotransduction in living cells. Physiol Rev 2001;81:685–740.
14.
Hinojosa R: Transport of ferritin across Reissner’s membrane. Acta Otolaryngol 1971;292:6–27.
15.
Horner KC: Auditory and vestibular function in experimental hydrops. Otolaryngol Head Neck Surg 1995;112:84–89.
16.
Hudspeth AJ: Establishment of tight junctions between epithelial cells. Proc Natl Acad Sci 1975;72:2711–2713.
17.
Ilberg C: Elektronenmikroskopische Untersuchungen über Diffusion und Resorption von Thoriumdioxyd an der Meerschweinchenschnecke. Arch Klin Exp Nasen- und Kehlkopfheilk 1968;192:163–175.
18.
Jahnke K: Electronenmikroskopische Untersuchungen über die Permeabilitätsbarrieren des Innenohres. Arch Klin Ohr- Nas Kehlkopfheilk 1973;204:199–216.
19.
Jahnke K: The fine structure of freeze-fractured intercellular junctions in the guinea pig inner ear. Acta Otolaryngol 1975(suppl 336):1–40.
20.
Jahnke K, Morgenstern, Mori N: Freeze-fracture studies on the perilymph-endolymph barrier in experimentally induced hydrops. Arch Otorhinolaryngol 1985;241:175–181.
21.
Johnsson LG: Reissner’s membrane in the human cochlea. Ann Otol Laryngol 1971;80:425–438.
22.
Kakigi A, Takeda T: Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential. Hear Res 1998;116:113–118.
23.
Kimura RS: Experimental blockage of the endolymphatic duct and sac and its effect on the inner ear of the guinea pig. A study on endolymphatic hydrops. Ann Otol Rhinol Laryngol 1967;76:664–687.
24.
Kimura RS, Schuknecht HF: Membranous hydrops in the inner ear of the guinea pig after obliteration of the endolymphatic sac. Pract Otorhinolaryngol 1965;27:343–354.
25.
Lin JH, Weigel H, Cotrina ML, Liu S, Bueno E, Hansen AJ, Hansen TW, Goldman S, Nedergard M: Gap-junction-mediated propagation and amplification of cell injury. Nat Neurosci 1998;1:494–500.
26.
Ménière P: Pathologie auriculaire. Gaz Méd Paris 1861;16:597–601.
27.
Qvortrup K, Rostgaard J, Bretlau P: Ultrastructure of Reissner’s membrane in the rabbit. Eur Arch Otorhinolaryngol 1994;251:249–256.
28.
Rarey KE, Ross MD: A survey on the effects of loop diuretics on the zonulae occludentes of the perilymph-endolymph barrier by freeze fracture. Acta Otolaryngol 1982;94:307–316.
29.
Reissner E: De auris internae formatione; thesis, Dorpat, 1851.
30.
Ruding PR, Veldman JE,van Deelen GW, Smoorenburg GF, Huizing EH: Histopathological study of experimentally induced endolymphatic hydrops with emphasis on Reissner’s membrane. Arch Otorhinolaryngol 1987;244:174–179.
31.
Sakagami M, Fukazawa K, Kitamura K, Doi K, Mori N, Matsunaga T: Transport of HRP through Reissner’s membrane in experimental endolymphatic hydrops. Acta Otolaryngol 1991;111:872–878.
32.
Salt AN, Henson MM, Gewalt SL, Keating AW, DeMott JE, Henson OW Jr: Detection and quantification of endolymphatic hydrops in the guinea pig cochlea by magnetic resonance microscopy. Hear Res 1995;88:79–86.
33.
Schuknecht HF: Pathophysiology of Ménière’s disease. Otolaryngol Clin North Am 1975;8:507–514.
34.
Shinozaki N, Kimura RS: Scanning electron microscopic observations on the distended Reissner’s and saccular membranes in the guinea pig. Acta Otolaryngol 1980;90:370–384.
35.
Ulfendahl M, Flock Å, Khanna SM: A temporal bone preparation for the study of cochlear micromechanics at the cellular level. Hear Res 1989;40:55–64.
36.
Wangemann P, Schacht J: Homeostatic mechanisms in the cochlea; in Dallos P, Popper AN, Fay RR (eds): The Cochlea. Berlin, Springer 1996, pp 157–185.
37.
Watanuki K, Kawamoto K, Katagiri S: Structure of Reissner’s membrane in the cat cochlea – a light microscopic structure. Tohoku J Exp Med 1971;104:121–127.
38.
Yeh TH, Herman P, Tsai MC, Tran Ba Huy P, Abbeele T: A cationic nonselective stretch-activated channel in the Reissner’s membrane of the guinea pig cochlea. Am J Physiol 1998;274:566–576.
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2003
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