... to detect inflammatory activity and altered monocyte function at an early stage of renal disease, we studied patients with CKD stages 2-3 over 5 years. Methods: The expression of adhesion molecules on monocytes at resting state and after stimulation with formyl-methionyl-leucyl-phenylalanine (fMLP), as well...

...Liesbeth Viaene; Pieter Evenepoel; Björn Meijers; Dirk Vanderschueren; Lut Overbergh; Chantal Mathieu Background: Local production of 1,25-dihydroxyvitamin D (1,25(OH) 2 D) regulated by the CYP27B1 enzyme in monocytes contributes to the immunomodulatory effects of vitamin D. Uremia suppresses renal...

...William A. Briggs; Kenneth S. Wielechowski; Sudesh K. Mahajan; Franklin D. McDonald Most studies relating alterations in insulin receptor binding to abnormalities of glucose tolerance in humans have used peripheral blood monocytes for the in vitro assay of insulin-binding kinetics. Studies...

... and cytokine production. Methods: To assess the ET-binding affinity of sevelamer hydrochloride, purified Escherichia coli ET was incubated with sevelamer hydrochloride (0–50 mg/ml). After incubation, ET was measured in supernatants. In addition, THP-1-derived monocytes were co-incubated with supernatants...

...Stefan H. Jacobson; Britta Hylander; Pia Thylén; Joachim Lundahl Aims: We studied markers of monocyte activation, i.e., the cell surface expression of CD11b and CD62L, and the serum concentrations of monocyte chemotactic protein 1 (MCP-1; a monocyte-specific chemoattractant) and soluble vascular...

.... Results: Significantly increased spontaneous and lipopolysaccharide (LPS)-stimulated production of IL-18 was detected in peripheral blood monocyte (PBM) cultures of MCNS patients with the nephrotic syndrome (NS) as compared with those of normal controls. Moreover, when individual MCNS patients were...