Background: The CD34 antigen is a marker of precursor myeloid cells and endothelial cells, but also of a subset of spindle cells. Methods: To establish the possible role of CD34+ cells in the progression of glomerulonephritis, 30 renal biopsies were investigated. Immunohistochemistry for CD34 and smooth muscle actin (SMA) were performed by standard methods. The results were assessed semiquantitatively. The relative interstitial volume (RIV) was evaluated by the point counting method. Clinical data such as sex, age, serum creatinine and urea levels were taken into account. Results: CD34+ spindle cells and SMA+ myofibroblasts were present in the renal interstitium in all but one case. In membranous, membranoproliferative and segmental-proliferative glomerulonephritis, CD34+ cells were seen in clusters, while in IgA nephropathy and in segmental and focal glomerulosclerosis, single, scattered cells were seen. The density of CD34+ cells showed a positive correlation with the myofibroblast density and the RIV. Serum creatinine and urea levels were associated with the RIV of the kidney and to a smaller degree with the number of myofibroblasts and CD34+ cells. Conclusion: These results show that in glomerulonephritis, CD34+ spindle cells appear in the interstitium. At present, their possible role can only be speculated.

Humphreys TR, Monteiro MR, Murphy GF: Mast cells and dendritic cells in basal cell carcinoma stroma. Dermatol Surg 2000;26:200–204.
Barth PJ, Ebrahimsade S, Hellinger A, Moll R, Ramaswamy A: CD34+ fibrocytes in neoplastic and inflammatory pancreatic lesions. Virchows Arch 2002;440:128–133.
Izumi M, Suda K, Torii A, Inadama E: Pancreatic ductal myofibroblasts. Proliferative patterns in various pathologic situations. Virchows Arch 2001;438:442–450.
Barth PJ, Ebrahimsade S, Ramaswamy A, Moll R: CD34+ fibrocytes in invasive ductal carcinoma, ductal carcinoma in situ, and benign breast lesions. Virchows Arch 2002;440:298–303.
Yamazaki K, Eyden BP: Ultrastructural and immunohistochemical observations on intralobular fibroblasts of human breast, with observations on the CD34 antigen. J Submicrosc Cytol Pathol 1995;27:309–323.
Nakayama H, Enzan H, Miyazaki E, Kuroda N, Naruse K, Kiyoku H, Toi M, Hiroi M: CD34 positive stromal cells in gastric adenocarcinomas. J Clin Pathol 2001;54:846–848.
Narvaez D, Kanitakis J, Faure M, Claudy A: Immunohistochemical study of CD34-positive dendritic cells of human dermis. Am J Dermatopathol 1996;18:283–288.
Nickoloff BJ: The human progenitor cell antigen (CD34) is localized on endothelial cells, dermal dendritic cells, and perifollicular cells in formalin-fixed normal skin, and on proliferating endothelial cells and stromal spindle-shaped sells in Kaposi’s sarcoma. Arch Dermatol 1991;127:523–529.
Barth PJ, Ramaswamy A, Moll R: CD34+ fibrocytes in normal cervical stroma, cervical endothelial neoplasia III, and invasive squamous cell carcinoma of the cervix uteri. Virchows Arch 2002;441:564–568.
Bucala R, Spiegel LA, Chesney J, Hogan M, Cerami A: Circulating fibrocytes define a new leukocyte subpopulation that mediates tissue repair. Mol Med 1994;1:71–81.
Churg J, Bernstein J, Glassock RJ: The processing and examination of renal biopsies; in Churg J, Bernstein J, Glassock RJ (eds): Renal Disease. Classification and Atlas of Glomerular Diseases, ed 2. Tokyo, Igaku-Shoin, 1995, p 515.
Katsenelson NS, Shurin GV, Bykovskaia SN, Shogan J, Shupin MR: Human small cell lung carcinoma and carcinoid tumor regulate dendritic cell maturation and function. Mod Pathol 2001;14:40–45.
Crosby HA, Kelly DA, Strain AJ: Human hepatic stem-like cells isolated using c-kit or CD34 can differentiate into biliary epithelium. Gastroenterology 2000;120:534–544.
Al-Awqati Q, Olivier JA: Stem cells in the kidney. Kidney Int 2002;61:387–395.
Forbes SJ, Poulsom R, Wright NA: Hepatic and renal differentiation from blood-borne stem cells. Gene Ther 2002;9:625–630.
Gupta S, Verfaillie C, Chmielewski D, Kim Y, Rosenberg ME: A role for extrarenal cells in the regeneration following acute renal failure. Kidney Int 2002;62:1285–1290.
Ito T, Suzuki A, Imai E, Okabe M, Hori M: Bone marrow is a reservoir of repopulating mesangial cells during glomerular remodeling. J Am Soc Nephrol 2001;12:2625–2635.
Kirchmann TT, Prieto VG, Smoller BR: CD34 staining pattern distinguishes basal cell carcinoma from trichoepithelioma. Arch Dermatol 1994;130:589–592.
Nakayama H, Enzan H, Miyazaki E, Kuroda N, Naruse K, Hiroi M: Differential expression of CD34 in normal colorectal tissue, peritumoral inflammatory tissue, and tumor stroma. J Clin Pathol 2000;53:626–629.
Cummings TJ, Burchette JL, McLendon RE: CD34 and dural fibroblasts: The relationship to solitary fibrous tumor and meningioma. Acta Neuropathol (Berl) 2001;102:349–354.
Nakayama H, Naruse K, Miyazaki E, Hiroi M, Kiyoku H, Kuroda N, Enzan H: The specific distribution of dendritic interstitial cells at the tumor border of major salivary gland pleomorphic adenomas. Mod Pathol 1999;12:445–449.
Naruse K, Fujieda M, Miyazaki E, Hayashi Y, Kuroda N, Nakayama H, Kiyoku H, Hiroi M, Kurashige T, Enzan H: CD34 expression as a novel marker of transformed mesangial cells in biopsied glomerular diseases. J Pathol 1999;189:105–111.
Chebotareva NV, Proppe D, Rudolf P, Kozlowskaia LV: Klinicheskoe znachenie ekspressii gladkomyshechonogo actina-alfa i CD34 antigena mezanigialnymi kletkami pri glomerulonefrite. (Clinical significance of expression of smooth muscle actin-alpha and CD34 antigen in mesangial cells in glomerulonephritis). Ter Arkh 2002;74:27–31.
Badid C, Vincent M, Fouque D, Laville M, Desmouliere A: Myofibroblast: A prognostic marker and target cell in progressive renal disease. Ren Fail 2001;23:543–549.
Nath KA: The tubulointerstitium in progressive renal disease. Kidney Int 1998;54:992–994.
Couser WG, Johnson RJ: Mechanisms of progressive renal disease in glomerulonephritis. Am J Kidney Dis 1994;23:193–198.
D’Amico G: Tubulo-interstitial damage in glomerular diseases: Its role in the progression of the renal damage. Nephrol Dial Transplant 1998;13(suppl 1):80–85.
Aiba S, Tabata N, Ohtani H, Tagami H: CD34+ spindle-shaped cells selectively disappear from the skin lesion of scleroderma. Arch Dermatol 1994;130:593–597.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.