.... Oligoclonal hematopoiesis evolved over time with 1-2 clones demonstrated 5-11 months after reconstitution. Comparable results were obtained with embryonic fetal liver HSCs. Incubation of bone marrow HSCs with adherent stromal cells rather than growth factors produced less efficient gene transfer...

...Pamela S. Becker Autologous hematopoietic cells have been used as targets of gene transfer, with applications in inherited disorders, cell therapy, and acquired immunodeficiency. The types of cells include hematopoietic progenitor cells, lymphocytes, and mesenchymal stem cells. The inherited...

... numbers of leukemic cells are readily obtained from bone marrow or peripheral blood. Yet, maintenance of leukemic cells in culture – acute B cell leukemia in particular – and high efficiency gene transfer remain a challenge. In vaccine strategies based on DCs, generation of vaccine cells ex vivo...

...Hatem E. Sabaawy; Tim Farley; Tauseef Ahmed; Eric Feldman; Nader G. Abraham Gene transfer has advantages in the treatment of a variety of disorders due to its selective expression within specific mammalian cells including the most primitive stem cells and cancer cells. Several investigators have...

...Silke Thomsen; Birgit Vogt; Dorothee von Laer; Christoph Heberlein; Alan Rein; Wolfram Ostertag; Carol Stocking Hematopoietic stem cells (HSC) are an important target for retroviral gene transfer. However, transduction efficiency in these HSC is extremely low compared to fibroblasts or more mature...